ID A0A100W685_9MYCO Unreviewed; 300 AA.
AC A0A100W685;
DT 13-APR-2016, integrated into UniProtKB/TrEMBL.
DT 13-APR-2016, sequence version 1.
DT 28-JUN-2023, entry version 32.
DE RecName: Full=Alkylmercury lyase {ECO:0000256|ARBA:ARBA00018180};
DE EC=4.99.1.2 {ECO:0000256|ARBA:ARBA00013237};
DE AltName: Full=Organomercurial lyase {ECO:0000256|ARBA:ARBA00031271};
GN ORFNames=RMCB_6478 {ECO:0000313|EMBL:GAS92382.1};
OS Mycolicibacterium brisbanense.
OC Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales; Mycobacteriaceae;
OC Mycolicibacterium.
OX NCBI_TaxID=146020 {ECO:0000313|EMBL:GAS92382.1, ECO:0000313|Proteomes:UP000069620};
RN [1] {ECO:0000313|EMBL:GAS92382.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JCM15654 {ECO:0000313|EMBL:GAS92382.1};
RA Katahira K., Ogura Y., Gotoh Y., Hayashi T.;
RT "Draft Genome Sequences of Five Rapidly Growing Mycobacterium Species, M.
RT thermoresistibile, M. fortuitum subsp. acetamidolyticum, M. canariasense,
RT M. brisbanense, and M. novocastrense.";
RL Genome Announc. 4:e00322-16(2016).
CC -!- FUNCTION: Cleaves the carbon-mercury bond of organomercurials such as
CC phenylmercuric acetate. One product is Hg(2+), which is subsequently
CC detoxified by the mercuric reductase. {ECO:0000256|ARBA:ARBA00025326}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=an alkylmercury + H(+) = an alkane + Hg(2+);
CC Xref=Rhea:RHEA:18777, ChEBI:CHEBI:15378, ChEBI:CHEBI:16793,
CC ChEBI:CHEBI:18310, ChEBI:CHEBI:83725; EC=4.99.1.2;
CC Evidence={ECO:0000256|ARBA:ARBA00000165};
CC -!- SIMILARITY: Belongs to the MerB family.
CC {ECO:0000256|ARBA:ARBA00009443}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:GAS92382.1}.
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DR EMBL; BCSX01000054; GAS92382.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A100W685; -.
DR STRING; 146020.RMCB_6478; -.
DR OrthoDB; 7185309at2; -.
DR Proteomes; UP000069620; Unassembled WGS sequence.
DR GO; GO:0018836; F:alkylmercury lyase activity; IEA:UniProtKB-EC.
DR GO; GO:0046413; P:organomercury catabolic process; IEA:InterPro.
DR GO; GO:0046689; P:response to mercury ion; IEA:UniProtKB-KW.
DR Gene3D; 3.30.450.410; -; 1.
DR InterPro; IPR004927; MerB.
DR InterPro; IPR024259; MerB_HTH_dom.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR Pfam; PF12324; HTH_15; 1.
DR Pfam; PF03243; MerB; 1.
DR PRINTS; PR01699; ORGNOHGLYASE.
DR SUPFAM; SSF160387; NosL/MerB-like; 1.
DR SUPFAM; SSF46785; Winged helix' DNA-binding domain; 1.
PE 3: Inferred from homology;
KW Lyase {ECO:0000256|ARBA:ARBA00023239};
KW Mercuric resistance {ECO:0000256|ARBA:ARBA00022466};
KW Mercury {ECO:0000256|ARBA:ARBA00022914}.
FT DOMAIN 113..170
FT /note="Alkylmercury lyase helix-turn-helix"
FT /evidence="ECO:0000259|Pfam:PF12324"
SQ SEQUENCE 300 AA; 31986 MW; 2F1B3EF5C94434E0 CRC64;
MRIELLTAPD CPHAASARQL VTDCLSSMAL DLPIIERVGR YPSPTVLVDG ADVMCPAATV
LAADACRLDL PTPERLLTAL RANSADHLRD AARKEPLMCA DPGADSVSRD TDQKTGRHLM
RTAVRLLAGG GPVTVADLCA AAGVDRADLT TAPAGRDIEY DDHHRIIGWG LTLNPSPHVY
IVDGQHLYTW CAADTLLFPV ILDRRAQIES RCPTTDIVVS FTVDPRSGVT DLSPSTAVIS
IPGSQTMDSA AVRATICDPG RFFANPQAAD DWLTAHPGGT VLPVANAYPQ LRLISGRLLD
//
