ID A0A101F6C1_9FIRM Unreviewed; 621 AA.
AC A0A101F6C1;
DT 13-APR-2016, integrated into UniProtKB/TrEMBL.
DT 13-APR-2016, sequence version 1.
DT 27-MAR-2024, entry version 24.
DE RecName: Full=starch synthase {ECO:0000256|ARBA:ARBA00012588};
DE EC=2.4.1.21 {ECO:0000256|ARBA:ARBA00012588};
DE Flags: Fragment;
GN ORFNames=XD63_1455 {ECO:0000313|EMBL:KUK31277.1};
OS Thermoanaerobacterales bacterium 50_218.
OC Bacteria; Bacillota; Clostridia; Thermoanaerobacterales.
OX NCBI_TaxID=1635288 {ECO:0000313|EMBL:KUK31277.1, ECO:0000313|Proteomes:UP000053634};
RN [1] {ECO:0000313|Proteomes:UP000053634}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=26787827; DOI=10.1128/mbio.01669-15;
RA Hu P., Tom L., Singh A., Thomas B.C., Baker B.J., Piceno Y.M.,
RA Andersen G.L., Banfield J.F.;
RT "Genome-Resolved Metagenomic Analysis Reveals Roles for Candidate Phyla and
RT Other Microbial Community Members in Biogeochemical Transformations in Oil
RT Reservoirs.";
RL MBio 7:e01669-15(2015).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[(1->4)-alpha-D-glucosyl](n) + ADP-alpha-D-glucose = [(1->4)-
CC alpha-D-glucosyl](n+1) + ADP + H(+); Xref=Rhea:RHEA:18189, Rhea:RHEA-
CC COMP:9584, Rhea:RHEA-COMP:9587, ChEBI:CHEBI:15378, ChEBI:CHEBI:15444,
CC ChEBI:CHEBI:57498, ChEBI:CHEBI:456216; EC=2.4.1.21;
CC Evidence={ECO:0000256|ARBA:ARBA00001478};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 57 family.
CC {ECO:0000256|ARBA:ARBA00006821, ECO:0000256|RuleBase:RU361196}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KUK31277.1}.
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DR EMBL; LGFL01000052; KUK31277.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A101F6C1; -.
DR Proteomes; UP000053634; Unassembled WGS sequence.
DR GO; GO:0003844; F:1,4-alpha-glucan branching enzyme activity; IEA:InterPro.
DR GO; GO:0030979; P:alpha-glucan biosynthetic process; IEA:InterPro.
DR CDD; cd10792; GH57N_AmyC_like; 1.
DR Gene3D; 1.20.1430.10; Families 57/38 glycoside transferase, middle domain; 1.
DR Gene3D; 3.40.50.2000; Glycogen Phosphorylase B; 1.
DR Gene3D; 3.20.110.10; Glycoside hydrolase 38, N terminal domain; 1.
DR InterPro; IPR037090; 57_glycoside_trans_central.
DR InterPro; IPR015293; BE_C.
DR InterPro; IPR040042; Branching_enz_MT3115-like.
DR InterPro; IPR011330; Glyco_hydro/deAcase_b/a-brl.
DR InterPro; IPR027291; Glyco_hydro_38_N_sf.
DR InterPro; IPR028995; Glyco_hydro_57/38_cen_sf.
DR InterPro; IPR004300; Glyco_hydro_57_N.
DR InterPro; IPR013534; Starch_synth_cat_dom.
DR PANTHER; PTHR41695; 1,4-ALPHA-GLUCAN BRANCHING ENZYME RV3031-RELATED; 1.
DR PANTHER; PTHR41695:SF1; 1,4-ALPHA-GLUCAN BRANCHING ENZYME RV3031-RELATED; 1.
DR Pfam; PF09210; BE_C; 1.
DR Pfam; PF03065; Glyco_hydro_57; 1.
DR Pfam; PF08323; Glyco_transf_5; 1.
DR SUPFAM; SSF88688; Families 57/38 glycoside transferase middle domain; 1.
DR SUPFAM; SSF88713; Glycoside hydrolase/deacetylase; 1.
DR SUPFAM; SSF53756; UDP-Glycosyltransferase/glycogen phosphorylase; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism {ECO:0000256|RuleBase:RU361196};
KW Glycosyltransferase {ECO:0000256|ARBA:ARBA00022676};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 7..394
FT /note="Glycoside hydrolase family 57 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF03065"
FT DOMAIN 427..527
FT /note="1,4-alpha-glucan branching enzyme C-terminal"
FT /evidence="ECO:0000259|Pfam:PF09210"
FT DOMAIN 548..606
FT /note="Starch synthase catalytic"
FT /evidence="ECO:0000259|Pfam:PF08323"
FT ACT_SITE 188
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR640042-1"
FT ACT_SITE 350
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR640042-1"
FT BINDING 240
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR640042-2"
FT BINDING 257
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR640042-2"
FT BINDING 407
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR640042-2"
FT BINDING 467
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR640042-2"
FT NON_TER 621
FT /evidence="ECO:0000313|EMBL:KUK31277.1"
SQ SEQUENCE 621 AA; 71474 MW; 300C5AE65365B2F1 CRC64;
MSSGYLALVL HAHLPYVRHP ECQTAVAERW LWEALTESYI PLLQTFFRLA DEKIPFRITL
SLSPPLISML GDPLLQDRYW KHLHLSLELG AKEIARNKGN PQLKNLAEFY LERLEGIKKF
YEEWGHDLIS AFRRLHRTGH IEVITTAATH GYLPLMVTEE ARKAQIAISV ESFEKWFGFS
PKGFWLPECG YKPGIEKILG EFGIKYFIVE THGILHASPP PKDGVYAPLL TSSGVAAFGR
DPDSSRQVWS SREGYPGDFC YREFYRDIGY ELDYDYLAPY LPGDGVRVDT GFKYYRITGP
GEEKQYYDPH LAMEKVRVHA GHFLFAREKQ IEHLSSHLSP KKPIIVAPYD AELFGHWWFE
GPLWLGELIR RIPLQQTFSL TTPSDYLEEA RNKGWQLQQA ELPESSWGLG GYHEVWLGPA
NDWIYRHLHL AEKRMVELAK SYPDSYGLQR RALNQAAREL LLAQSSDWAF IMHAGTVVEY
ARQRVNDHLG RFTLLYHQIR NNDLDQEMLE FIEWLDPIFP CLDYRVYADK IKKSAKNEVL
VPRSSKGRVL VLTWEFPPRS IGGLSRHVAG LTQALAEAGE AVDVIAPWVP GAPTFSQNGL
LRVHRVRVDA EDIHDFLDWV F
//
