ID A0A101G6Q0_9FIRM Unreviewed; 457 AA.
AC A0A101G6Q0;
DT 13-APR-2016, integrated into UniProtKB/TrEMBL.
DT 13-APR-2016, sequence version 1.
DT 27-MAR-2024, entry version 24.
DE SubName: Full=Radical SAM domain protein {ECO:0000313|EMBL:KUK52769.1};
GN ORFNames=XD78_1737 {ECO:0000313|EMBL:KUK52769.1};
OS Desulfotomaculum sp. 46_296.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Desulfotomaculaceae;
OC Desulfotomaculum.
OX NCBI_TaxID=1635262 {ECO:0000313|EMBL:KUK52769.1, ECO:0000313|Proteomes:UP000053813};
RN [1] {ECO:0000313|Proteomes:UP000053813}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=26787827; DOI=10.1128/mbio.01669-15;
RA Hu P., Tom L., Singh A., Thomas B.C., Baker B.J., Piceno Y.M.,
RA Andersen G.L., Banfield J.F.;
RT "Genome-Resolved Metagenomic Analysis Reveals Roles for Candidate Phyla and
RT Other Microbial Community Members in Biogeochemical Transformations in Oil
RT Reservoirs.";
RL MBio 7:e01669-15(2015).
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KUK52769.1}.
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DR EMBL; LGFZ01000061; KUK52769.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A101G6Q0; -.
DR STRING; 1635262.XD78_1737; -.
DR PATRIC; fig|1635262.3.peg.268; -.
DR Proteomes; UP000053813; Unassembled WGS sequence.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0031419; F:cobalamin binding; IEA:InterPro.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR CDD; cd01335; Radical_SAM; 1.
DR CDD; cd02068; radical_SAM_B12_BD; 1.
DR Gene3D; 3.40.50.280; Cobalamin-binding domain; 1.
DR Gene3D; 3.80.30.20; tm_1862 like domain; 1.
DR InterPro; IPR006158; Cobalamin-bd.
DR InterPro; IPR036724; Cobalamin-bd_sf.
DR InterPro; IPR006638; Elp3/MiaA/NifB-like_rSAM.
DR InterPro; IPR034466; Methyltransferase_Class_B.
DR InterPro; IPR007197; rSAM.
DR InterPro; IPR023404; rSAM_horseshoe.
DR PANTHER; PTHR43409; ANAEROBIC MAGNESIUM-PROTOPORPHYRIN IX MONOMETHYL ESTER CYCLASE-RELATED; 1.
DR PANTHER; PTHR43409:SF12; BLL1977 PROTEIN; 1.
DR Pfam; PF02310; B12-binding; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR SFLD; SFLDG01082; B12-binding_domain_containing; 1.
DR SFLD; SFLDG01123; methyltransferase_(Class_B); 1.
DR SMART; SM00729; Elp3; 1.
DR SUPFAM; SSF52242; Cobalamin (vitamin B12)-binding domain; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR PROSITE; PS51332; B12_BINDING; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
PE 4: Predicted;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Iron {ECO:0000256|ARBA:ARBA00022485};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00022485};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723}.
FT DOMAIN 7..133
FT /note="B12-binding"
FT /evidence="ECO:0000259|PROSITE:PS51332"
FT DOMAIN 172..389
FT /note="Radical SAM core"
FT /evidence="ECO:0000259|PROSITE:PS51918"
SQ SEQUENCE 457 AA; 53105 MW; B0A8B6AB02B72B73 CRC64;
MKITLIEPQP PSQHVFSDIK LPRLGLPLLG AVLARLGHDV RIYVEDFAPV NMRRVLESDL
VGFSTTTSTV PAAYEIADQV RRERKDIPIV FGGSHVTFLP EEALEHSDYC VRGEGEHTIA
ELVASLESGS DPESIQGLSY KKNGLIFHNP DRPLEKDLDS LPFPDFTLVE GHEKMRITPI
ATSRGCPYNC RFCSVIQMFG RKYRIRDIDN VIEEINEKQP KRIFFYDDNF TADRHRTIKM
LQGFIDKCPK FLWSAQVRAE VARDEEILKL MYKSGCRIVY IGFESINQET LAAYNKKLDV
SEIVSSIKTI HKHGIRIHGM FVIGADEDTE KTAKSTVDFA LKNRIDTIQL MILTPLPGTP
YFDDLVQEDR IISSNWSHYD AHHVVYQPKQ MSPYWLQKET LKHMKRFYSL KNCVRQACRF
DIANLYFRFM GYRILFNWYR NPLNRNFMKK LRQEFVY
//
