ID A0A101JNZ7_9ACTN Unreviewed; 982 AA.
AC A0A101JNZ7;
DT 13-APR-2016, integrated into UniProtKB/TrEMBL.
DT 13-APR-2016, sequence version 1.
DT 27-MAR-2024, entry version 35.
DE RecName: Full=Multifunctional fusion protein {ECO:0000256|HAMAP-Rule:MF_00254, ECO:0000256|HAMAP-Rule:MF_00255};
DE Includes:
DE RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000256|HAMAP-Rule:MF_00255};
DE EC=6.1.1.14 {ECO:0000256|HAMAP-Rule:MF_00255};
DE AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000256|HAMAP-Rule:MF_00255};
DE Short=GlyRS {ECO:0000256|HAMAP-Rule:MF_00255};
DE Includes:
DE RecName: Full=Glycine--tRNA ligase alpha subunit {ECO:0000256|HAMAP-Rule:MF_00254};
DE AltName: Full=Glycyl-tRNA synthetase alpha subunit {ECO:0000256|HAMAP-Rule:MF_00254};
GN Name=glyQ {ECO:0000256|HAMAP-Rule:MF_00254};
GN Synonyms=glyS {ECO:0000256|HAMAP-Rule:MF_00255};
GN ORFNames=ADL15_25705 {ECO:0000313|EMBL:KUL29973.1};
OS Actinoplanes awajinensis subsp. mycoplanecinus.
OC Bacteria; Actinomycetota; Actinomycetes; Micromonosporales;
OC Micromonosporaceae; Actinoplanes.
OX NCBI_TaxID=135947 {ECO:0000313|EMBL:KUL29973.1, ECO:0000313|Proteomes:UP000053244};
RN [1] {ECO:0000313|EMBL:KUL29973.1, ECO:0000313|Proteomes:UP000053244}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NRRL B-16712 {ECO:0000313|EMBL:KUL29973.1,
RC ECO:0000313|Proteomes:UP000053244};
RA Gilbert D.G.;
RL Submitted (OCT-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC EC=6.1.1.14; Evidence={ECO:0000256|ARBA:ARBA00000201,
CC ECO:0000256|HAMAP-Rule:MF_00255};
CC -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC {ECO:0000256|HAMAP-Rule:MF_00255}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00255}.
CC -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC {ECO:0000256|ARBA:ARBA00008226, ECO:0000256|HAMAP-Rule:MF_00255}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KUL29973.1}.
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DR EMBL; LLZH01000268; KUL29973.1; -; Genomic_DNA.
DR RefSeq; WP_067696329.1; NZ_LLZH01000268.1.
DR AlphaFoldDB; A0A101JNZ7; -.
DR OrthoDB; 9775440at2; -.
DR Proteomes; UP000053244; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR HAMAP; MF_00254; Gly_tRNA_synth_alpha; 1.
DR HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR InterPro; IPR045864; aa-tRNA-synth_II/BPL/LPL.
DR InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR InterPro; IPR002310; Gly-tRNA_ligase_asu.
DR NCBIfam; TIGR00388; glyQ; 1.
DR NCBIfam; TIGR00211; glyS; 1.
DR PANTHER; PTHR30075:SF2; GLYCINE--TRNA LIGASE, CHLOROPLASTIC_MITOCHONDRIAL 2; 1.
DR PANTHER; PTHR30075; GLYCYL-TRNA SYNTHETASE; 1.
DR Pfam; PF02091; tRNA-synt_2e; 1.
DR Pfam; PF02092; tRNA_synt_2f; 1.
DR PRINTS; PR01044; TRNASYNTHGA.
DR SUPFAM; SSF55681; Class II aaRS and biotin synthetases; 1.
DR SUPFAM; SSF109604; HD-domain/PDEase-like; 1.
DR PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 2.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146,
KW ECO:0000256|HAMAP-Rule:MF_00255};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP-
KW Rule:MF_00255}; Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00255};
KW Ligase {ECO:0000256|ARBA:ARBA00022598, ECO:0000256|HAMAP-Rule:MF_00255};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_00255};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917, ECO:0000256|HAMAP-
KW Rule:MF_00255}; Reference proteome {ECO:0000313|Proteomes:UP000053244}.
SQ SEQUENCE 982 AA; 106246 MW; 98DC3A006D573A35 CRC64;
MLTMQEALTR LTAYWTEQGC LIVQPMNTEV GAGTLNPATF LRVLGPEPWR VVYVEPSVRP
DDSRYGENPN RLQTHTQMQV ILKPDPGNPQ ELYLGSLAAL GIDVAAHDVR FVEDNWASPA
LGAWGLGWEV WLDGLEITQF TYFQQAGGLN LDPPSVEITY GIERIIMALQ DKQHFKEIEY
APGVSYGEVF GQSEYEMSRY YLDDADIDAN RRLLELYAGE AQRMIDAGLP VPAHSYVLKC
SQAFNVLDSR GAVSTAERAS EFARMRRLAG EVAKLWVARR AELEHPLGLV ADLEPARPAG
AAQTGDRPRT LLFEIGAEEL PPAELRNARA QLQRLLTEGL AATRLEHGEI RVFGTPRRLI
AVVQAVAARE EDFVRTVRGP KTSAPAKAIE GFARGHGVTP EELATEEFNG VPHLVLHKQE
SGGAAPQVLT AVLAKVVGGL RSAKNMRWND PKLAFSRPLR WLTALWGDDV VPVTVSTLAA
GRQTRLLRTA ATPIVEIASA ETFVETLGVN GIVADHDDRR ELIVVGAQDL VYPDGRIDVT
GESALIDQIT DLVEQPLPLL GTFDEGYLTL PDAVLTTVMR KHQRYLPVRD EDGKLLPMFV
TVANGPVDVE LVRTGNEAVL RARYEDARFF YRADLDTAPA DMRAKLTRLT FTDKLGSMAD
RADRIGTLAG QLGERLGLGS PALTRAAELV KFDLGSQLVT EMTSLAGVMA RDYALRGGES
RAVAEAVFEA ELPRNTGDAL PRTIPGALLS LADRLDLVTG LAATVGLPTG SSDPFAVRRA
VLGLLAVHRS TPEFASLSLV EALSLAAAAQ PVPVSAEVLA ATGDFLAKRL EQMLTEEGQP
VDRVRAVLPH FARPSVADTL LAQLATAVGD ADFASVTAAI QRARRIVPEG TPATYDAGAL
KEPAELALHD AVTTVRAALD PALIDLPRFV TATGPLVGPV NTFFDDVLVM ADDPAIRAAR
LGLLATVRDL GDGLLDWPAL RL
//
