ID   A0A101KS53_RHILI        Unreviewed;       515 AA.
AC   A0A101KS53;
DT   13-APR-2016, integrated into UniProtKB/TrEMBL.
DT   13-APR-2016, sequence version 1.
DT   27-MAR-2024, entry version 28.
DE   RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900};
DE            EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900};
GN   ORFNames=AU467_23575 {ECO:0000313|EMBL:KUM25978.1};
OS   Rhizobium loti (Mesorhizobium loti).
OC   Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
OC   Phyllobacteriaceae; Mesorhizobium.
OX   NCBI_TaxID=381 {ECO:0000313|EMBL:KUM25978.1, ECO:0000313|Proteomes:UP000053176};
RN   [1] {ECO:0000313|EMBL:KUM25978.1, ECO:0000313|Proteomes:UP000053176}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=UFLA 01-765 {ECO:0000313|EMBL:KUM25978.1,
RC   ECO:0000313|Proteomes:UP000053176};
RA   Rangel W.M., Thijs S., Longatti S.M., Moreira F.M., Weyens N.,
RA   Vangronsveld J., Van Hamme J.D., Bottos E.M., Rineau F.;
RT   "Draft genome sequence of Mesorhizobium sp. UFLA 01-765, a multitolerant
RT   efficient symbiont and plant-growth promoting strain isolated from Zn-
RT   mining soil using Leucaena leucocephala as a trap plant.";
RL   Submitted (DEC-2015) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC         N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC         homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC         COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC         Evidence={ECO:0000256|ARBA:ARBA00001279};
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:KUM25978.1}.
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DR   EMBL; LPWA01000110; KUM25978.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A101KS53; -.
DR   REBASE; 147159; M.Msp765ORF23575P.
DR   OrthoDB; 9806213at2; -.
DR   Proteomes; UP000053176; Unassembled WGS sequence.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR   GO; GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IEA:UniProtKB-EC.
DR   CDD; cd02440; AdoMet_MTases; 1.
DR   Gene3D; 1.20.1260.30; -; 1.
DR   Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR   InterPro; IPR022749; D12N6_MeTrfase_N.
DR   InterPro; IPR003356; DNA_methylase_A-5.
DR   InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   InterPro; IPR038333; T1MK-like_N_sf.
DR   PANTHER; PTHR42998:SF1; TYPE I RESTRICTION ENZYME HINDI METHYLASE SUBUNIT; 1.
DR   PANTHER; PTHR42998; TYPE I RESTRICTION ENZYME HINDVIIP M PROTEIN-RELATED; 1.
DR   Pfam; PF12161; HsdM_N; 1.
DR   Pfam; PF02384; N6_Mtase; 1.
DR   PRINTS; PR00507; N12N6MTFRASE.
DR   SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR   PROSITE; PS00092; N6_MTASE; 1.
PE   4: Predicted;
KW   Methyltransferase {ECO:0000313|EMBL:KUM25978.1};
KW   Transferase {ECO:0000313|EMBL:KUM25978.1}.
FT   DOMAIN          15..142
FT                   /note="N6 adenine-specific DNA methyltransferase N-
FT                   terminal"
FT                   /evidence="ECO:0000259|Pfam:PF12161"
FT   DOMAIN          154..474
FT                   /note="DNA methylase adenine-specific"
FT                   /evidence="ECO:0000259|Pfam:PF02384"
SQ   SEQUENCE   515 AA;  57196 MW;  5F23356316D39E09 CRC64;
     MAGQKDNNSD LNFTADLFRA ADKLRGNLEP SEYKHVALGL IFLKYISDAF EAQRAKLAAD
     KYADPEDPEE YLAESVFWVP KEARWSSLQG NAKRPEIGKL IDEAMEAIEK APSNDGLKGV
     LPKNYARPTL NKTMLGELID LFSNIGMHDG TDKAKDLLGR VYEYFLSGFA SSEGKRGGEF
     FTPRSVVRTL VEMLEPYKGR VYDPCCGSGG MFVQSEKFIE EHGGRRHDIA VYGQEINHTT
     WRLAKMNLAV QGIDADIRWN NEGSFHRDEL PDLKADFILA NPPFNISDWG GERLADDVRW
     RYGAPPKGNA NFAWLQHILH HLAPRGTGGV VLANGSMSSQ QSGEGDIRKA MIEADVVDCM
     VALPGQLFFS TQIPACLWIL ARDKSANGHR ERRGEILFID ARKLGFMADR VRKEFSADDI
     GKIAGAYHRW RAKDDAGGYA DEPGFCKSAT LAEVRGHGCV LTPGRYVGAA DAEDDGVSFA
     EKFDDLREQL GTQFVAGREL EERITAALSE IEYDV
//