ID A0A101NE02_9ACTN Unreviewed; 499 AA.
AC A0A101NE02;
DT 13-APR-2016, integrated into UniProtKB/TrEMBL.
DT 13-APR-2016, sequence version 1.
DT 24-JAN-2024, entry version 18.
DE SubName: Full=Glycoside hydrolase {ECO:0000313|EMBL:KUM91217.1};
GN ORFNames=AQI88_38030 {ECO:0000313|EMBL:KUM91217.1};
OS Streptomyces cellostaticus.
OC Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC Streptomycetaceae; Streptomyces.
OX NCBI_TaxID=67285 {ECO:0000313|EMBL:KUM91217.1, ECO:0000313|Proteomes:UP000054241};
RN [1] {ECO:0000313|EMBL:KUM91217.1, ECO:0000313|Proteomes:UP000054241}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 40189 {ECO:0000313|EMBL:KUM91217.1,
RC ECO:0000313|Proteomes:UP000054241};
RA Ruckert C., Winkler A., Kalinowski J., Kampfer P., Glaeser S.;
RT "Draft genome sequence of Streptomyces cellostaticus DSM 40189, type strain
RT for the species Streptomyces cellostaticus.";
RL Submitted (OCT-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 43 family.
CC {ECO:0000256|ARBA:ARBA00009865, ECO:0000256|RuleBase:RU361187}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KUM91217.1}.
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DR EMBL; LMWL01000082; KUM91217.1; -; Genomic_DNA.
DR RefSeq; WP_067009177.1; NZ_KQ948051.1.
DR AlphaFoldDB; A0A101NE02; -.
DR STRING; 67285.AQI88_38030; -.
DR OrthoDB; 9801455at2; -.
DR Proteomes; UP000054241; Unassembled WGS sequence.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd18617; GH43_XynB-like; 1.
DR Gene3D; 2.60.120.200; -; 1.
DR InterPro; IPR013320; ConA-like_dom_sf.
DR InterPro; IPR041542; GH43_C2.
DR InterPro; IPR006710; Glyco_hydro_43.
DR InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR PANTHER; PTHR42812; BETA-XYLOSIDASE; 1.
DR PANTHER; PTHR42812:SF12; BETA-XYLOSIDASE-RELATED; 1.
DR Pfam; PF17851; GH43_C2; 1.
DR Pfam; PF04616; Glyco_hydro_43; 1.
DR SUPFAM; SSF75005; Arabinanase/levansucrase/invertase; 1.
DR SUPFAM; SSF49899; Concanavalin A-like lectins/glucanases; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU361187};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU361187};
KW Reference proteome {ECO:0000313|Proteomes:UP000054241}.
FT DOMAIN 306..495
FT /note="Beta-xylosidase C-terminal Concanavalin A-like"
FT /evidence="ECO:0000259|Pfam:PF17851"
FT ACT_SITE 17
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT ACT_SITE 174
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT SITE 126
FT /note="Important for catalytic activity, responsible for
FT pKa modulation of the active site Glu and correct
FT orientation of both the proton donor and substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-2"
SQ SEQUENCE 499 AA; 53881 MW; 046BA7D9FF5CD47C CRC64;
MSDTGPANPV IPGFHPDPSI CRVGDDYYLA CSSFEYFPGV PLFHSRDLVH WTQIGNVLDR
PSQLRLAADV PSSAGIYAPT LRHHDGRFWL IVTNVSGGGG NLLVTATDPA GPWSDPIWAP
GVPGIDPDLA WDEDGTCWCT FAGVGQVRID PHTGETYGPP RKLWSGAPGD KAPEAPHLYR
IGDYWYLLIA VGGTERGHGV AIARGLTPSG PFEPCPANPI LTHRSTDHPV QNTGHADLVQ
APDGSWWMVL LGVRPGGGTP GWHVLGRETF LAPVTWVDGW PVVGEVALDL PELPWPVVPG
PVEEPRDDFD LSELRPSWIS VRDRPAEHCT TKERPGWLTL RARGVDLDEP DVVFTGRRQQ
HLSCRARTLV DPSDGTGGLA VRLDERHHYA IEASGSEVRV IARVGSLRTV VAAQPVPDGP
VVLGLTITDP PPPHDARTGP DVVSLGFEQP DGTFTVLATL DGRYLSTEVA GGFTGRVIGM
YAAAGTVHFD WFAYEPLRP
//
