ID A0A101WHZ9_9FIRM Unreviewed; 804 AA.
AC A0A101WHZ9;
DT 13-APR-2016, integrated into UniProtKB/TrEMBL.
DT 13-APR-2016, sequence version 1.
DT 27-MAR-2024, entry version 32.
DE SubName: Full=CRISPR-associated protein Cas3 {ECO:0000313|EMBL:KUO72928.1};
GN ORFNames=APF81_10695 {ECO:0000313|EMBL:KUO72928.1};
OS Desulfosporosinus sp. BRH_c37.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Desulfitobacteriaceae;
OC Desulfosporosinus.
OX NCBI_TaxID=1734396 {ECO:0000313|EMBL:KUO72928.1, ECO:0000313|Proteomes:UP000053148};
RN [1] {ECO:0000313|EMBL:KUO72928.1, ECO:0000313|Proteomes:UP000053148}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=BRH_c37 {ECO:0000313|EMBL:KUO72928.1};
RA Bagnoud A., Chourey K., Hettich R.L., De Bruijn I., Andersson A.F.,
RA Leupin O.X., Schwyn B., Bernier-Latmani R.;
RT "Microbial metabolic network in the subsurface.";
RL Submitted (OCT-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: In the N-terminal section; belongs to the CRISPR-associated
CC nuclease Cas3-HD family. {ECO:0000256|ARBA:ARBA00006847}.
CC -!- SIMILARITY: In the central section; belongs to the CRISPR-associated
CC helicase Cas3 family. {ECO:0000256|ARBA:ARBA00009046}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KUO72928.1}.
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DR EMBL; LOEW01000228; KUO72928.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A101WHZ9; -.
DR Proteomes; UP000053148; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR CDD; cd09641; Cas3''_I; 1.
DR CDD; cd17930; DEXHc_cas3; 1.
DR Gene3D; 1.10.3210.30; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR006483; CRISPR-assoc_Cas3_HD.
DR InterPro; IPR038257; CRISPR-assoc_Cas3_HD_sf.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR006474; Helicase_Cas3_CRISPR-ass_core.
DR InterPro; IPR018222; Nuclear_transport_factor_2_euk.
DR InterPro; IPR027417; P-loop_NTPase.
DR NCBIfam; TIGR01587; cas3_core; 1.
DR NCBIfam; TIGR01596; cas3_HD; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF109604; HD-domain/PDEase-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51643; HD_CAS3; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS50177; NTF2_DOMAIN; 1.
PE 3: Inferred from homology;
KW Antiviral defense {ECO:0000256|ARBA:ARBA00023118};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741}.
FT DOMAIN 9..206
FT /note="HD Cas3-type"
FT /evidence="ECO:0000259|PROSITE:PS51643"
FT DOMAIN 260..446
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 639..787
FT /note="NTF2"
FT /evidence="ECO:0000259|PROSITE:PS50177"
SQ SEQUENCE 804 AA; 91531 MW; 97DF6612987C0D2F CRC64;
MLCAHINPLT LMEQSVKKHL NNVCILSREY GSKISLGATG ELIGILHDLG KGTRKFDSYI
RYCSKHPNDK SLKGSINHST AGAKYIYDAF YNSTDQYQKF TAQIISLAIC SHHGGLIDCL
DLGGMDKFTD RMNPDKDILY DEALNYFNEK CLELSQIKDL FNKATEEIKS MFIKINKIDG
SGKFGRFAAG VLEKYLFSCV IDADRYDTYI FMEGKERKPN IDKSALWNEL SDVLETKLES
YPKETKIELL RDEVSLACKN FAENSPGIYQ LSVPTGGGKT LSSLRYALEH AKKFDKDRIF
YIIPFTTIID QNAKEIKDIL GHEDMILEHH SNLILDYSQD NDEELEDYKL LTERWDSPII
LTTMVQFLNT LFSGGTQDAR RIHNLANSVI IFDEIQAIPI KCINIFNSAI NFLAYICNAT
VILCTATQPL LEKTEMPLKL SERPNIISDI HEKFKQFKRV NLVPKILVGG YSAISLKDFV
LDKMDQVKSV LVILNTKNSA KEVFNELRVA NKELPKEKQY TIFHLSTSMC PSHRMSVLQK
MREKLGQERV ICISTQLIEA GVNISFGCVV RSLAGLDNIA QAAGRCNRHG EKECSDVYIV
NVEGENVSKL VDIKIAQECT SRVLRDFEEN RNLFDNDLLS QKTMERYYKY YFHERRNQMN
YTMSKPNSDR SLYDILSGNN EAANEFNSRN GYQSKLMLKQ AFKTAGNEFQ AIDENTTGII
VPYKEGKELI TLINGACSLS ELKQYLKRAQ QFSVNLFETD KRKLEYMGGF IGLKNNSILV
LKDEFYNDDV GVTFDKAPMG FCNA
//
