ID A0A101X8H1_9CREN Unreviewed; 337 AA.
AC A0A101X8H1;
DT 13-APR-2016, integrated into UniProtKB/TrEMBL.
DT 13-APR-2016, sequence version 1.
DT 27-MAR-2024, entry version 27.
DE RecName: Full=Probable tRNA pseudouridine synthase B {ECO:0000256|HAMAP-Rule:MF_01081};
DE EC=5.4.99.25 {ECO:0000256|HAMAP-Rule:MF_01081};
DE AltName: Full=tRNA pseudouridine(55) synthase {ECO:0000256|HAMAP-Rule:MF_01081};
DE Short=Psi55 synthase {ECO:0000256|HAMAP-Rule:MF_01081};
DE AltName: Full=tRNA pseudouridylate synthase {ECO:0000256|HAMAP-Rule:MF_01081};
DE AltName: Full=tRNA-uridine isomerase {ECO:0000256|HAMAP-Rule:MF_01081};
GN Name=truB {ECO:0000256|HAMAP-Rule:MF_01081};
GN ORFNames=AT709_00245 {ECO:0000313|EMBL:KUO86677.1};
OS Caldivirga sp. MG_3.
OC Archaea; Thermoproteota; Thermoprotei; Thermoproteales; Thermoproteaceae;
OC Caldivirga.
OX NCBI_TaxID=1714256 {ECO:0000313|EMBL:KUO86677.1, ECO:0000313|Proteomes:UP000054806};
RN [1] {ECO:0000313|EMBL:KUO86677.1, ECO:0000313|Proteomes:UP000054806}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Jay Z.J., Beam J.P., Kozubal M.A., Jennings R.D.E., Rusch D.B.,
RA Inskeep W.P.;
RT "The distribution, diversity and function of predominant Thermoproteales in
RT high-temperature environments of Yellowstone National Park.";
RL Submitted (DEC-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Could be responsible for synthesis of pseudouridine from
CC uracil-55 in the psi GC loop of transfer RNAs. {ECO:0000256|HAMAP-
CC Rule:MF_01081}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=uridine(55) in tRNA = pseudouridine(55) in tRNA;
CC Xref=Rhea:RHEA:42532, Rhea:RHEA-COMP:10101, Rhea:RHEA-COMP:10102,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.25;
CC Evidence={ECO:0000256|HAMAP-Rule:MF_01081};
CC -!- SIMILARITY: Belongs to the pseudouridine synthase TruB family. Type 2
CC subfamily. {ECO:0000256|HAMAP-Rule:MF_01081}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KUO86677.1}.
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DR EMBL; LOCB01000001; KUO86677.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A101X8H1; -.
DR Proteomes; UP000054806; Unassembled WGS sequence.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0106029; F:tRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:UniProtKB-UniRule.
DR CDD; cd21148; PUA_Cbf5; 1.
DR Gene3D; 3.30.2350.10; Pseudouridine synthase; 1.
DR Gene3D; 2.30.130.10; PUA domain; 1.
DR HAMAP; MF_01081; TruB_arch; 1.
DR InterPro; IPR012960; Dyskerin-like.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR002501; PsdUridine_synth_N.
DR InterPro; IPR002478; PUA.
DR InterPro; IPR015947; PUA-like_sf.
DR InterPro; IPR036974; PUA_sf.
DR InterPro; IPR004802; tRNA_PsdUridine_synth_B_fam.
DR InterPro; IPR026326; TruB_arch.
DR InterPro; IPR032819; TruB_C.
DR NCBIfam; TIGR00425; CBF5; 1.
DR PANTHER; PTHR23127; CENTROMERE/MICROTUBULE BINDING PROTEIN CBF5; 1.
DR PANTHER; PTHR23127:SF0; H_ACA RIBONUCLEOPROTEIN COMPLEX SUBUNIT DKC1; 1.
DR Pfam; PF08068; DKCLD; 1.
DR Pfam; PF01472; PUA; 1.
DR Pfam; PF16198; TruB_C_2; 1.
DR Pfam; PF01509; TruB_N; 1.
DR SMART; SM01136; DKCLD; 1.
DR SMART; SM00359; PUA; 1.
DR SUPFAM; SSF55120; Pseudouridine synthase; 1.
DR SUPFAM; SSF88697; PUA domain-like; 1.
DR PROSITE; PS50890; PUA; 1.
PE 3: Inferred from homology;
KW Isomerase {ECO:0000256|ARBA:ARBA00023235, ECO:0000256|HAMAP-Rule:MF_01081};
KW tRNA processing {ECO:0000256|HAMAP-Rule:MF_01081}.
FT DOMAIN 9..57
FT /note="Dyskerin-like"
FT /evidence="ECO:0000259|SMART:SM01136"
FT DOMAIN 244..318
FT /note="PUA"
FT /evidence="ECO:0000259|SMART:SM00359"
FT ACT_SITE 76
FT /note="Nucleophile"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01081"
SQ SEQUENCE 337 AA; 37695 MW; B45C07869EB13B7E CRC64;
MLVEVNCSGN RAVRVKVDEG TDPRYGTEPY RRSIRDYVNF GFIILDKPRG PTSSEVVSWV
KKLLNIGRAG HSGTLDPGVS GVLPVALGDS TKVLQGIANA EKEYVGVMLL HSQVDQNRVK
AVFKEFTGRI YQRPPLRSAV KRRIRVKTVY DLDILEFDGR YVLFRARVES GTYIRKLCYD
IGEVLGVGAS MRELRRVRVG CFREEDAVNL ETLREAYALW RDYGDEALLR RVIRPVEEMV
IHLPRIYVRD SAVDAIAHGA SLAAPGVAKV EEGIVKNQLV ALMTLKGELI ALAEATASTE
EILSMNRGIV AKPTRVIMPR GVYPSTWRRG QQKPESK
//
