ID A0A126RPF8_9SPHN Unreviewed; 523 AA.
AC A0A126RPF8;
DT 11-MAY-2016, integrated into UniProtKB/TrEMBL.
DT 11-MAY-2016, sequence version 1.
DT 27-MAR-2024, entry version 38.
DE RecName: Full=Transcription termination/antitermination protein NusA {ECO:0000256|HAMAP-Rule:MF_00945};
GN Name=nusA {ECO:0000256|HAMAP-Rule:MF_00945,
GN ECO:0000313|EMBL:AMK24078.1};
GN ORFNames=K426_15730 {ECO:0000313|EMBL:AMK24078.1};
OS Sphingobium sp. TKS.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
OC Sphingomonadaceae; Sphingobium.
OX NCBI_TaxID=1315974 {ECO:0000313|EMBL:AMK24078.1, ECO:0000313|Proteomes:UP000060779};
RN [1] {ECO:0000313|Proteomes:UP000060779}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=TKS {ECO:0000313|Proteomes:UP000060779};
RA Tabata M.;
RT "Whole genome sequence of gamma-HCH degrading bacterium Sphingobium sp.
RT TKS.";
RL Submitted (APR-2013) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:AMK24078.1, ECO:0000313|Proteomes:UP000060779}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=TKS {ECO:0000313|EMBL:AMK24078.1,
RC ECO:0000313|Proteomes:UP000060779};
RX PubMed=27056231;
RA Tabata M., Ohhata S., Kawasumi T., Nikawadori Y., Kishida K., Sato T.,
RA Ohtsubo Y., Tsuda M., Nagata Y.;
RT "Complete Genome Sequence of a gamma-Hexachlorocyclohexane Degrader,
RT Sphingobium sp. Strain TKS, Isolated from a gamma-Hexachlorocyclohexane-
RT Degrading Microbial Community.";
RL Genome Announc. 4:0-0(2016).
CC -!- FUNCTION: Participates in both transcription termination and
CC antitermination. {ECO:0000256|HAMAP-Rule:MF_00945}.
CC -!- SUBUNIT: Monomer. Binds directly to the core enzyme of the DNA-
CC dependent RNA polymerase and to nascent RNA. {ECO:0000256|HAMAP-
CC Rule:MF_00945}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00945}.
CC -!- SIMILARITY: Belongs to the NusA family. {ECO:0000256|HAMAP-
CC Rule:MF_00945}.
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DR EMBL; CP005083; AMK24078.1; -; Genomic_DNA.
DR RefSeq; WP_066558966.1; NZ_CP005083.1.
DR AlphaFoldDB; A0A126RPF8; -.
DR STRING; 1315974.K426_15730; -.
DR KEGG; spht:K426_15730; -.
DR PATRIC; fig|1315974.3.peg.3295; -.
DR OrthoDB; 9807233at2; -.
DR Proteomes; UP000060779; Chromosome 1.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR GO; GO:0000166; F:nucleotide binding; IEA:InterPro.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003746; F:translation elongation factor activity; IEA:UniProtKB-KW.
DR GO; GO:0006353; P:DNA-templated transcription termination; IEA:UniProtKB-UniRule.
DR GO; GO:0031564; P:transcription antitermination; IEA:UniProtKB-UniRule.
DR CDD; cd02134; KH-II_NusA_rpt1; 1.
DR CDD; cd22529; KH-II_NusA_rpt2; 1.
DR CDD; cd04455; S1_NusA; 1.
DR Gene3D; 3.30.300.20; -; 2.
DR Gene3D; 1.10.150.20; 5' to 3' exonuclease, C-terminal subdomain; 2.
DR Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1.
DR Gene3D; 3.30.1480.10; NusA, N-terminal domain; 1.
DR HAMAP; MF_00945_B; NusA_B; 1.
DR InterPro; IPR010995; DNA_repair_Rad51/TF_NusA_a-hlx.
DR InterPro; IPR004087; KH_dom.
DR InterPro; IPR015946; KH_dom-like_a/b.
DR InterPro; IPR025249; KH_dom_NusA-like.
DR InterPro; IPR009019; KH_sf_prok-type.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR030842; NusA_bac.
DR InterPro; IPR036555; NusA_N_sf.
DR InterPro; IPR003029; S1_domain.
DR InterPro; IPR013735; TF_NusA_N.
DR InterPro; IPR010214; Tscrpt_termin_fac_NusA_C_rpt.
DR InterPro; IPR010213; Tscrpt_termination_fac_NusA.
DR NCBIfam; TIGR01953; NusA; 1.
DR NCBIfam; TIGR01954; nusA_Cterm_rpt; 1.
DR PANTHER; PTHR22648; TRANSCRIPTION TERMINATION FACTOR NUSA; 1.
DR PANTHER; PTHR22648:SF0; TRANSCRIPTION TERMINATION_ANTITERMINATION PROTEIN NUSA; 1.
DR Pfam; PF14520; HHH_5; 1.
DR Pfam; PF13184; KH_5; 1.
DR Pfam; PF08529; NusA_N; 1.
DR Pfam; PF00575; S1; 1.
DR SMART; SM00322; KH; 2.
DR SMART; SM00316; S1; 1.
DR SUPFAM; SSF50249; Nucleic acid-binding proteins; 1.
DR SUPFAM; SSF54814; Prokaryotic type KH domain (KH-domain type II); 2.
DR SUPFAM; SSF47794; Rad51 N-terminal domain-like; 2.
DR SUPFAM; SSF69705; Transcription factor NusA, N-terminal domain; 1.
DR PROSITE; PS50084; KH_TYPE_1; 1.
DR PROSITE; PS50126; S1; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490, ECO:0000256|HAMAP-Rule:MF_00945};
KW Elongation factor {ECO:0000313|EMBL:AMK24078.1};
KW Protein biosynthesis {ECO:0000313|EMBL:AMK24078.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000060779};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884, ECO:0000256|HAMAP-
KW Rule:MF_00945};
KW Transcription {ECO:0000256|ARBA:ARBA00023163, ECO:0000256|HAMAP-
KW Rule:MF_00945};
KW Transcription antitermination {ECO:0000256|ARBA:ARBA00022814,
KW ECO:0000256|HAMAP-Rule:MF_00945};
KW Transcription regulation {ECO:0000256|ARBA:ARBA00023015, ECO:0000256|HAMAP-
KW Rule:MF_00945};
KW Transcription termination {ECO:0000256|ARBA:ARBA00022472,
KW ECO:0000256|HAMAP-Rule:MF_00945}.
FT DOMAIN 142..206
FT /note="S1 motif"
FT /evidence="ECO:0000259|PROSITE:PS50126"
SQ SEQUENCE 523 AA; 58066 MW; D8E1C741B8261D2B CRC64;
MANAISANKA ELLAIANSVA SEKMIDKAIV IEAMEDAIQR AARARYGAEN DIRAKLDPET
GDLRLWRVVE VVEAVDDYFK QVDLRQAQKL KKDAVIGDFI VDPLPPIDLG RIDAQSAKQV
IFQKVRDAER ERQHEEFKDR VGEIITGVVK SVEFGHVVVN LGRAEGVIRR DQQIPREVVR
VGDRIRSVIL NVRRENRGPQ IFLSRAHPEF MKKLFAQEVP EIYDGVIEIK AAARDPGSRA
KIGVISRDSS IDPVGACVGM KGSRVQAVVQ EMQGEKIDII PWSEDTATFV VNALQPAQVA
RVVIDEEEER IEVVVPDDQL SLAIGRRGQN VRLASQLTGK AIDIMTEADA SEKRQKEFVA
RSELFQNELD VDETLSQLLV AEGFGELEEV AYVGVEELAA IEGFDEDLAA ELQSRAQEAL
DRREAAAREE RQALGVEDAL AGMPHLTEAM LVTLGKAGIK TLDDLADLAT DELIAKKRVD
QRRRRNESSE DKGGILAEYG LNEEQGNEII MAARAHWFED EEA
//
