ID A0A127B198_9BACT Unreviewed; 525 AA.
AC A0A127B198;
DT 08-JUN-2016, integrated into UniProtKB/TrEMBL.
DT 08-JUN-2016, sequence version 1.
DT 27-MAR-2024, entry version 25.
DE RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900};
DE EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900};
GN ORFNames=TH61_08535 {ECO:0000313|EMBL:AMM51213.1};
OS Rufibacter sp. DG15C.
OC Bacteria; Bacteroidota; Cytophagia; Cytophagales; Hymenobacteraceae;
OC Rufibacter.
OX NCBI_TaxID=1379909 {ECO:0000313|EMBL:AMM51213.1, ECO:0000313|Proteomes:UP000070672};
RN [1] {ECO:0000313|EMBL:AMM51213.1, ECO:0000313|Proteomes:UP000070672}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DG15C {ECO:0000313|EMBL:AMM51213.1,
RC ECO:0000313|Proteomes:UP000070672};
RA Kim M.K., Srinivasan S., Lee J.-J.;
RT "Rufibacter sp. DG15C whole genome sequencing.";
RL Submitted (JAN-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC Evidence={ECO:0000256|ARBA:ARBA00001279};
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DR EMBL; CP010776; AMM51213.1; -; Genomic_DNA.
DR RefSeq; WP_066508284.1; NZ_CP010776.1.
DR AlphaFoldDB; A0A127B198; -.
DR STRING; 1379909.TH61_08535; -.
DR REBASE; 139277; M.Rsp15ORF8535P.
DR KEGG; rud:TH61_08535; -.
DR PATRIC; fig|1379909.4.peg.1845; -.
DR OrthoDB; 9814572at2; -.
DR Proteomes; UP000070672; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR GO; GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IEA:UniProtKB-EC.
DR GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1260.30; -; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR022749; D12N6_MeTrfase_N.
DR InterPro; IPR003356; DNA_methylase_A-5.
DR InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR InterPro; IPR004546; Restrct_endonuc_T1M.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR038333; T1MK-like_N_sf.
DR NCBIfam; TIGR00497; hsdM; 1.
DR PANTHER; PTHR42933:SF1; SITE-SPECIFIC DNA-METHYLTRANSFERASE (ADENINE-SPECIFIC); 1.
DR PANTHER; PTHR42933; SLR6095 PROTEIN; 1.
DR Pfam; PF12161; HsdM_N; 1.
DR Pfam; PF02384; N6_Mtase; 1.
DR PRINTS; PR00507; N12N6MTFRASE.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS00092; N6_MTASE; 1.
PE 4: Predicted;
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Endonuclease {ECO:0000313|EMBL:AMM51213.1};
KW Hydrolase {ECO:0000313|EMBL:AMM51213.1};
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603};
KW Nuclease {ECO:0000313|EMBL:AMM51213.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000070672};
KW Restriction system {ECO:0000256|ARBA:ARBA00022747};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 9..171
FT /note="N6 adenine-specific DNA methyltransferase N-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF12161"
FT DOMAIN 184..485
FT /note="DNA methylase adenine-specific"
FT /evidence="ECO:0000259|Pfam:PF02384"
FT COILED 491..518
FT /evidence="ECO:0000256|SAM:Coils"
SQ SEQUENCE 525 AA; 59514 MW; E710E11A8F565CED CRC64;
MSDNNKKQLE QQLWNIANTL RGKMDADEFR DYILGFIFYK YLSEKMELYA NKILTEDGLT
YLDIDEATEE GQEYLEAIKE EAVHSLGYFL KPSELFSEMA KKGNSENKEE GASNFILDEL
TQVLKHIEQS TMGTESEDDF NKLFEDLDLT STKLGRTEAA KNDLIAKVLG HLDKIDFKLQ
DTEADVLGDA YEYLIGQFAS GAGKKAGEFY TPQEVSKVLA KLVTTGKTKL KSVYDPTCGS
GSLLLRVAKE VQEVSKFYGQ ELNRTTYNLA RMNMILHDVH FQKFDIRQDD TLEHPQHLGQ
EFEAIVANPP FSAQWSANPL HMSDDRFSQY GKLAPSSKAD FAFVQHMVHH LAENGTMAVV
LPHGVLFRGG AEAHIRQYLI ADRNYLDAVI GLPAGIFYGT GIPTCILVFK KCREQADNIL
FIEASQHYGK EKTQNYLRIE DIEKITYTYR ERCAEDKYSY LAPLSEVKEN DFNLNIPRYV
DTFAEVKAID LKEIATALAA LEIEMQEADN TIDAFCDELG IPKPF
//
