ID A0A136IUX6_9PEZI Unreviewed; 880 AA.
AC A0A136IUX6;
DT 11-MAY-2016, integrated into UniProtKB/TrEMBL.
DT 11-MAY-2016, sequence version 1.
DT 27-MAR-2024, entry version 22.
DE RecName: Full=Beta-mannosidase B {ECO:0000256|ARBA:ARBA00041069};
DE EC=3.2.1.25 {ECO:0000256|ARBA:ARBA00012754};
DE AltName: Full=Mannanase B {ECO:0000256|ARBA:ARBA00041614};
GN ORFNames=Micbo1qcDRAFT_122651 {ECO:0000313|EMBL:KXJ88717.1};
OS Microdochium bolleyi.
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Xylariomycetidae; Xylariales; Microdochiaceae; Microdochium.
OX NCBI_TaxID=196109 {ECO:0000313|EMBL:KXJ88717.1, ECO:0000313|Proteomes:UP000070501};
RN [1] {ECO:0000313|Proteomes:UP000070501}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=J235TASD1 {ECO:0000313|Proteomes:UP000070501};
RG DOE Joint Genome Institute;
RA David A.S., May G., Haridas S., Lim J., Wang M., Labutti K., Lipzen A.,
RA Barry K., Grigoriev I.V.;
RT "Draft genome sequence of Microdochium bolleyi, a fungal endophyte of
RT beachgrass.";
RL Submitted (FEB-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal, non-reducing beta-D-mannose residues
CC in beta-D-mannosides.; EC=3.2.1.25;
CC Evidence={ECO:0000256|ARBA:ARBA00000829};
CC -!- PATHWAY: Glycan metabolism; N-glycan degradation.
CC {ECO:0000256|ARBA:ARBA00004740}.
CC -!- SUBUNIT: Homodimer. {ECO:0000256|ARBA:ARBA00011738}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000256|ARBA:ARBA00004613}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 2 family. Beta-
CC mannosidase B subfamily. {ECO:0000256|ARBA:ARBA00038429}.
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DR EMBL; KQ964257; KXJ88717.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A136IUX6; -.
DR STRING; 196109.A0A136IUX6; -.
DR InParanoid; A0A136IUX6; -.
DR OrthoDB; 2504097at2759; -.
DR UniPathway; UPA00280; -.
DR Proteomes; UP000070501; Unassembled WGS sequence.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0004567; F:beta-mannosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR Gene3D; 2.60.120.260; Galactose-binding domain-like; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 2.
DR InterPro; IPR036156; Beta-gal/glucu_dom_sf.
DR InterPro; IPR041625; Beta-mannosidase_Ig.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR006102; Glyco_hydro_2_Ig-like.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR041447; Mannosidase_ig.
DR PANTHER; PTHR43730; BETA-MANNOSIDASE; 1.
DR PANTHER; PTHR43730:SF1; BETA-MANNOSIDASE; 1.
DR Pfam; PF00703; Glyco_hydro_2; 1.
DR Pfam; PF17753; Ig_mannosidase; 1.
DR Pfam; PF17786; Mannosidase_ig; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF49303; beta-Galactosidase/glucuronidase domain; 2.
DR SUPFAM; SSF49785; Galactose-binding domain-like; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000313|EMBL:KXJ88717.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000070501};
KW Secreted {ECO:0000256|ARBA:ARBA00022525}.
FT DOMAIN 197..302
FT /note="Glycoside hydrolase family 2 immunoglobulin-like
FT beta-sandwich"
FT /evidence="ECO:0000259|Pfam:PF00703"
FT DOMAIN 686..788
FT /note="Mannosidase Ig/CBM-like"
FT /evidence="ECO:0000259|Pfam:PF17786"
FT DOMAIN 795..847
FT /note="Beta-mannosidase Ig-fold"
FT /evidence="ECO:0000259|Pfam:PF17753"
SQ SEQUENCE 880 AA; 100648 MW; 14E1024BECB459EC CRC64;
MQRRRHDLST GWTFRETGGT SQAWLPVAKV PSQVHLDLLA NDIIADPFCD LNELDVQWVA
ERDWTYRVQF VAPMTQGRLD GVTTTLVFEG LDTFARVTLN GVEILVSENM HLAHRIDVSN
LLRDEGENLL EIDFQSALLR GRELVRQHPE HDFFARQTEE SRIPVRKAQY NWGWDWGPIL
MTAGPWRPVY VEEYQARVDD LWARYELDTN LKTCAGVLSA RVDGVTSKDD LVILTLSFDD
VIVFEKNCTL DPSGVVATAF QIEDIKLWHV AGHGAQHRYR LSATIIRGKT ELDSRSKMIG
FRRCELIQEP DAFGKSFYFR INGVDTFAGG SCWIPADSFL ANMSSQKYFE WMKLLAESNQ
VMVRIWGGGI YEDDALFEAC DSLGLLVWHD FAFVCGNYPV FPAFLANIEA EARQNIRRLR
CHPSVVIWAG NNEDYQVQER YKLDYVFEDK DPESWKRSSF PARYIYEDLL PRILKEEDPQ
ATYHPGSPWG DGKHTTDPTV GDIHQWNIWH GQMSRYQDSH ELSGRFVSEF GMEAYPHFQT
IRRMVSKDEQ RFPGSMTMDF RNKAADHERR LNTYLCENFR VSYDLPSFTH LTQILQADTM
KYAYKAWRRM WGARGSRRCG GVLVWQLNDC WPTMSWAVVD YYMVKKPAFY SISRALRPLD
IGISRSCPEW TSGHADPTLL TAIPAFDVWV ASSCLEDVQV VVEVRLISIR SGKEIGPPIS
KIVFAQANST TEIFQNKRPQ LSSPEDVTSA HRRPFDPASH DPFVIYAILR RNDAVVATDT
AWPQPYKYLD FADRKVSVKM APSGDNITVT TERPVLGFVL EEVEGLTLSD NGFDIMPGEE
QVVKIKGGTL PAPGLDWTYV GAEHPGRVQM VYKPRLSSKM
//
