ID A0A136Q2D7_9FIRM Unreviewed; 484 AA.
AC A0A136Q2D7;
DT 08-JUN-2016, integrated into UniProtKB/TrEMBL.
DT 08-JUN-2016, sequence version 1.
DT 27-MAR-2024, entry version 24.
DE SubName: Full=Cytoplasmic alpha-amylase {ECO:0000313|EMBL:KXK64744.1};
GN ORFNames=HMPREF3293_01982 {ECO:0000313|EMBL:KXK64744.1};
OS Christensenella minuta.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Christensenellaceae;
OC Christensenella.
OX NCBI_TaxID=626937 {ECO:0000313|EMBL:KXK64744.1, ECO:0000313|Proteomes:UP000070366};
RN [1] {ECO:0000313|EMBL:KXK64744.1, ECO:0000313|Proteomes:UP000070366}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 22607 {ECO:0000313|EMBL:KXK64744.1,
RC ECO:0000313|Proteomes:UP000070366};
RA Wen L., He K., Yang H.;
RL Submitted (FEB-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC Evidence={ECO:0000256|ARBA:ARBA00001913};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KXK64744.1}.
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DR EMBL; LSZW01000063; KXK64744.1; -; Genomic_DNA.
DR RefSeq; WP_066518133.1; NZ_MAIR01000001.1.
DR AlphaFoldDB; A0A136Q2D7; -.
DR STRING; 626937.HMPREF3293_01982; -.
DR KEGG; cmiu:B1H56_05330; -.
DR PATRIC; fig|626937.4.peg.1960; -.
DR OrthoDB; 9805159at2; -.
DR Proteomes; UP000070366; Unassembled WGS sequence.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd11318; AmyAc_bac_fung_AmyA; 1.
DR Gene3D; 2.40.30.140; -; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR InterPro; IPR013776; A-amylase_thermo.
DR InterPro; IPR015237; Alpha-amylase_C_pro.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR43447; ALPHA-AMYLASE; 1.
DR PANTHER; PTHR43447:SF49; ALPHA-AMYLASE; 1.
DR Pfam; PF09154; Alpha-amy_C_pro; 1.
DR Pfam; PF00128; Alpha-amylase; 1.
DR PIRSF; PIRSF001021; Alph-amls_thrmst; 1.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
PE 4: Predicted;
KW Calcium {ECO:0000256|PIRSR:PIRSR001021-2};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR001021-2};
KW Reference proteome {ECO:0000313|Proteomes:UP000070366}.
FT DOMAIN 7..390
FT /note="Glycosyl hydrolase family 13 catalytic"
FT /evidence="ECO:0000259|SMART:SM00642"
FT ACT_SITE 233
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR001021-1"
FT ACT_SITE 263
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR001021-1"
FT BINDING 106
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000256|PIRSR:PIRSR001021-2"
FT BINDING 204
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000256|PIRSR:PIRSR001021-2"
FT BINDING 237
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000256|PIRSR:PIRSR001021-2"
FT BINDING 302
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000256|PIRSR:PIRSR001021-2"
SQ SEQUENCE 484 AA; 54380 MW; E5FF953866908680 CRC64;
MNTGKNGTMM QYFEWELPSG ILWKQAAEKA GELAGDGITA VWLPPAYKGS GGADDVGYAV
YDLYDLGEFG QKGSVATKYG TKEEYLRAVD ALHAAGLQVY ADIVLDHKMG ADGLEEVLAC
EFARENRYEQ IDGEETISAW TKFDFPGRNG VYSNFRWNSG HFAAIDRDEK TGRSAIFQFR
GKKWQDEVDK EFGNFEYLMG ASIDLSNEEV VRELTEWGKW YLHEVPLDGF RIDAVKHMRF
TFYSHWLAEL RKEAERELFA VGEYWNGDVA KLSNYIDITG GTLSLFDVPL HFRFRDAANA
GGAFDMRTIF DRTLVRENPL LAVPFVDNHD TQPGQSLESW VQDWFKPLAY ALILLRGDGY
PCVFYGDYYG IPTHSIMPKR QWLRPMIKAR ELLSYGKQHD YFDDPHLVGW TREGDEAQPG
SGLAVVMSNE VGGSKTMYVG ERLAGRAFYD VLGNRSGIVM AGADGNAVFS AGDGSVSVWA
PVLR
//
