ID   A0A139CL87_9EURY        Unreviewed;       335 AA.
AC   A0A139CL87;
DT   11-MAY-2016, integrated into UniProtKB/TrEMBL.
DT   11-MAY-2016, sequence version 1.
DT   24-JAN-2024, entry version 22.
DE   SubName: Full=Putative pyruvate formate lyase activating enzyme {ECO:0000313|EMBL:KXS42103.1};
GN   ORFNames=AWU59_1781 {ECO:0000313|EMBL:KXS42103.1};
OS   Methanolobus sp. T82-4.
OC   Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC   Methanosarcinales; Methanosarcinaceae; Methanolobus.
OX   NCBI_TaxID=1794908 {ECO:0000313|EMBL:KXS42103.1, ECO:0000313|Proteomes:UP000074030};
RN   [1] {ECO:0000313|EMBL:KXS42103.1, ECO:0000313|Proteomes:UP000074030}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=T82-4 {ECO:0000313|EMBL:KXS42103.1};
RA   Wolfe R., Daly R., Wrighton K.;
RT   "Methanolobus T82 Annotated.";
RL   Submitted (FEB-2016) to the EMBL/GenBank/DDBJ databases.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:KXS42103.1}.
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DR   EMBL; LSRV01000058; KXS42103.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A139CL87; -.
DR   STRING; 1794908.AWU59_1781; -.
DR   PATRIC; fig|1794908.3.peg.745; -.
DR   Proteomes; UP000074030; Unassembled WGS sequence.
DR   GO; GO:0051536; F:iron-sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   Gene3D; 3.20.20.70; Aldolase class I; 1.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR040085; MJ0674-like.
DR   InterPro; IPR007197; rSAM.
DR   PANTHER; PTHR43075; FORMATE LYASE ACTIVATING ENZYME, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G15630)-RELATED; 1.
DR   PANTHER; PTHR43075:SF1; FORMATE LYASE ACTIVATING ENZYME, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G15630)-RELATED; 1.
DR   Pfam; PF04055; Radical_SAM; 1.
DR   SFLD; SFLDS00029; Radical_SAM; 1.
DR   SFLD; SFLDG01099; Uncharacterised_Radical_SAM_Su; 1.
DR   SUPFAM; SSF102114; Radical SAM enzymes; 1.
PE   4: Predicted;
KW   Iron {ECO:0000256|ARBA:ARBA00023004};
KW   Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW   Lyase {ECO:0000313|EMBL:KXS42103.1};
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW   Pyruvate {ECO:0000313|EMBL:KXS42103.1};
KW   S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691}.
FT   DOMAIN          130..284
FT                   /note="Radical SAM core"
FT                   /evidence="ECO:0000259|Pfam:PF04055"
SQ   SEQUENCE   335 AA;  38843 MW;  C4BBBF5CBAFF4290 CRC64;
     MDRYSGIKAN TLPARNIILR HLDVSFETGL REEELWNIHD RGMKRYRNLE RKIFQTGKAV
     NLPEAEKNLL DLKIELSERI ISYCHLCQHR CMVDRPAGEK GFCRLTDVSR YAAEFMHMGE
     EPQLVPSHTI FFTGCVFSCV FCQNWDISTH PENGRQVIPE ELAHIIDNMR AKGSENVNFV
     TPTPHLMNVL KIVRNIEVNI PVIWNSNMYH SPESAKLLEG VVDLYLGDFK YGNDRCAFRY
     SKVRNYMDTV GQNFKAAARQ AEVLVRHLVM PGHLDCCTRP VMKWIAENTP QARLNLMFQY
     RPEHIACRYP EINRHLTNEE IKKALLIVKE EGLEG
//