ID A0A139SI51_9BACT Unreviewed; 433 AA.
AC A0A139SI51;
DT 11-MAY-2016, integrated into UniProtKB/TrEMBL.
DT 11-MAY-2016, sequence version 1.
DT 27-MAR-2024, entry version 24.
DE SubName: Full=Alpha-glucosidase/alpha-galactosidase {ECO:0000313|EMBL:KXU34160.1};
DE Flags: Fragment;
GN ORFNames=AXK12_07715 {ECO:0000313|EMBL:KXU34160.1};
OS Cephaloticoccus capnophilus.
OC Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
OC Cephaloticoccus.
OX NCBI_TaxID=1548208 {ECO:0000313|EMBL:KXU34160.1, ECO:0000313|Proteomes:UP000071392};
RN [1] {ECO:0000313|EMBL:KXU34160.1, ECO:0000313|Proteomes:UP000071392}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CV41 {ECO:0000313|EMBL:KXU34160.1,
RC ECO:0000313|Proteomes:UP000071392};
RA Wen L., He K., Yang H.;
RL Submitted (FEB-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC Evidence={ECO:0000256|ARBA:ARBA00001936};
CC -!- COFACTOR:
CC Name=NAD(+); Xref=ChEBI:CHEBI:57540;
CC Evidence={ECO:0000256|RuleBase:RU361152};
CC Note=Binds 1 NAD(+) per subunit. {ECO:0000256|RuleBase:RU361152};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 4 family.
CC {ECO:0000256|ARBA:ARBA00010141, ECO:0000256|RuleBase:RU361152}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KXU34160.1}.
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DR EMBL; LSZP01000060; KXU34160.1; -; Genomic_DNA.
DR RefSeq; WP_068713115.1; NZ_LSZP01000060.1.
DR AlphaFoldDB; A0A139SI51; -.
DR STRING; 1548208.AXK12_07715; -.
DR OrthoDB; 9808275at2; -.
DR Proteomes; UP000071392; Unassembled WGS sequence.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016616; F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR Gene3D; 3.90.1820.10; AglA-like glucosidase; 1.
DR InterPro; IPR019802; GlycHydrolase_4_CS.
DR InterPro; IPR001088; Glyco_hydro_4.
DR InterPro; IPR022616; Glyco_hydro_4_C.
DR InterPro; IPR015955; Lactate_DH/Glyco_Ohase_4_C.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR PANTHER; PTHR32092; 6-PHOSPHO-BETA-GLUCOSIDASE-RELATED; 1.
DR PANTHER; PTHR32092:SF6; ALPHA-GALACTOSIDASE; 1.
DR Pfam; PF02056; Glyco_hydro_4; 1.
DR Pfam; PF11975; Glyco_hydro_4C; 1.
DR PRINTS; PR00732; GLHYDRLASE4.
DR SUPFAM; SSF56327; LDH C-terminal domain-like; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
DR PROSITE; PS01324; GLYCOSYL_HYDROL_F4; 1.
PE 3: Inferred from homology;
KW Cobalt {ECO:0000256|PIRSR:PIRSR601088-3};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU361152};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU361152};
KW Iron {ECO:0000256|PIRSR:PIRSR601088-3};
KW Manganese {ECO:0000256|PIRSR:PIRSR601088-3};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR601088-3};
KW NAD {ECO:0000256|RuleBase:RU361152};
KW Nickel {ECO:0000256|PIRSR:PIRSR601088-3};
KW Reference proteome {ECO:0000313|Proteomes:UP000071392}.
FT DOMAIN 136..357
FT /note="Glycosyl hydrolase family 4 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF11975"
FT BINDING 90
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR601088-2"
FT BINDING 111
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000256|PIRSR:PIRSR601088-3"
FT BINDING 141
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000256|PIRSR:PIRSR601088-3"
FT SITE 51
FT /note="Increases basicity of active site Tyr"
FT /evidence="ECO:0000256|PIRSR:PIRSR601088-4"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:KXU34160.1"
SQ SEQUENCE 433 AA; 47734 MW; 089101F84A466697 CRC64;
LKAKVSSTLN RRTALRGANY VFCVVRTGGL EAFTHDIDIP LKYGIDQCVG DTLCAGGIMY
GQRGIAVLLD ICKDIREVAA PNCLLLNYAN PMAMMTWAAS VYGGVRCVGL CHGVQGGIHE
IARAIGASAK DLDVICVGIN HQTWFTEVKH NGVDVPMDRI LAGFENHPEA ARDEKVRIDM
MRRFGYFSTE SNGHLSEYLP WYRKRPAEIK KWIDTSTNAW IHGETGGYLR YVTEGRHWFE
EEFGEGRLPA TLEFTAENRS PEHGSYILEA METGRVYRGF FNLVNRNTIS NLPADAIIEA
PGFVDRFGFN MPQFGDLPLG CAAVCNASIS VQRMGAHAAA TGDINLLRQA MLMDPLVSAV
CNPPEVWQLV DEMLVAQAEW LPQYSEAIKG AKARLAKGPL IKTKAGYKGA ARKKIRTTAE
IRAQEKGNPR GGH
//
