ID   A0A143YNM3_9LACT        Unreviewed;       659 AA.
AC   A0A143YNM3;
DT   08-JUN-2016, integrated into UniProtKB/TrEMBL.
DT   08-JUN-2016, sequence version 1.
DT   27-MAR-2024, entry version 21.
DE   RecName: Full=pullulanase {ECO:0000256|ARBA:ARBA00024062};
DE            EC=3.2.1.41 {ECO:0000256|ARBA:ARBA00024062};
DE   AltName: Full=Alpha-dextrin endo-1,6-alpha-glucosidase {ECO:0000256|ARBA:ARBA00029618};
DE   AltName: Full=Pullulan 6-glucanohydrolase {ECO:0000256|ARBA:ARBA00031076};
GN   ORFNames=TR210_1242 {ECO:0000313|EMBL:CZQ94607.1};
OS   Trichococcus ilyis.
OC   Bacteria; Bacillota; Bacilli; Lactobacillales; Carnobacteriaceae;
OC   Trichococcus.
OX   NCBI_TaxID=640938 {ECO:0000313|EMBL:CZQ94607.1, ECO:0000313|Proteomes:UP000076878};
RN   [1] {ECO:0000313|EMBL:CZQ94607.1, ECO:0000313|Proteomes:UP000076878}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Trichococcus_R210 {ECO:0000313|EMBL:CZQ94607.1};
RA   Wen L., He K., Yang H.;
RL   Submitted (FEB-2016) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolysis of (1->6)-alpha-D-glucosidic linkages in pullulan,
CC         amylopectin and glycogen, and in the alpha- and beta-limit dextrins
CC         of amylopectin and glycogen.; EC=3.2.1.41;
CC         Evidence={ECO:0000256|ARBA:ARBA00023965};
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DR   EMBL; FJNB01000007; CZQ94607.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A143YNM3; -.
DR   STRING; 640938.TR210_1242; -.
DR   Proteomes; UP000076878; Unassembled WGS sequence.
DR   GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   CDD; cd11341; AmyAc_Pullulanase_LD-like; 1.
DR   CDD; cd02860; E_set_Pullulanase; 1.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR   Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR   InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR   InterPro; IPR004193; Glyco_hydro_13_N.
DR   InterPro; IPR013780; Glyco_hydro_b.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   InterPro; IPR013783; Ig-like_fold.
DR   InterPro; IPR014756; Ig_E-set.
DR   InterPro; IPR011840; PulA_typeI.
DR   NCBIfam; TIGR02104; pulA_typeI; 1.
DR   PANTHER; PTHR43002; GLYCOGEN DEBRANCHING ENZYME; 1.
DR   PANTHER; PTHR43002:SF11; PULLULANASE 1, CHLOROPLASTIC; 1.
DR   Pfam; PF00128; Alpha-amylase; 1.
DR   Pfam; PF02922; CBM_48; 1.
DR   SMART; SM00642; Aamy; 1.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR   SUPFAM; SSF81296; E set domains; 1.
PE   4: Predicted;
FT   DOMAIN          199..563
FT                   /note="Glycosyl hydrolase family 13 catalytic"
FT                   /evidence="ECO:0000259|SMART:SM00642"
SQ   SEQUENCE   659 AA;  75027 MW;  FB66EEF650274A6E CRC64;
     MYIQVDERKR TDVLSTTYYQ YDLDNLENRL EQFVQSPVFD KKYSFEGELG AIYTPQRTIL
     RLWAPTALSV EVIVYESLYR KEKKRHMMGK GGRGTHELIM IGDYAGTAYK YAITFPDGKV
     VETVDPYSYA TTANGERSVI LDAVNTNPER WGARLEPIAS PVDAIIYELH IRDFTISPTS
     GVTNKGKFLG VVEQGTLSAD GDKTGLDYLK ELGVTHVQFL PMADFYTVNE LKPLEQYNWG
     YDPQNFNVPE GSYATNPYQP EVRILEMKEM IQGLHDAGIR VIMDVVYNHV YLPKLHALEK
     TVPGYYFRKN ADGTLSNGTG VGNDTASERY MMRKYIIDSI TYWAKNFHLD GFRFDLMGIH
     DIETMNEIRI ALDAIDPSII MLGEGWNLNT NLPPSEKAVQ QNADRMPGVA HFNDALREAI
     KGSDFESGTD TGFVTGKPFM EGWVATNLQG GAYYPASRGN YINPAQMVQY VEAHDNLTLY
     DKLKISLPWD EEESRMRRHL LASSFVLLSQ GIPFIHAGQE FMRTKNGLEN SYNAPDSVNR
     MDWHRRSEMK PAVDYMKGLI ALRKQEPLFR LRTIEEVKEH MNILKADYQI VVYQLEDKGK
     LYYVIFNGQT NAIDFEVESG DYRVLIEDGT ASLAEPRLAE GISRIRVENL SVTVLVKDK
//