ID A0A151SE33_CAJCA Unreviewed; 968 AA.
AC A0A151SE33;
DT 08-JUN-2016, integrated into UniProtKB/TrEMBL.
DT 08-JUN-2016, sequence version 1.
DT 27-MAR-2024, entry version 26.
DE RecName: Full=phosphoenolpyruvate carboxylase {ECO:0000256|ARBA:ARBA00012305};
DE EC=4.1.1.31 {ECO:0000256|ARBA:ARBA00012305};
GN ORFNames=KK1_025033 {ECO:0000313|EMBL:KYP53033.1};
OS Cajanus cajan (Pigeon pea) (Cajanus indicus).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Cajanus.
OX NCBI_TaxID=3821 {ECO:0000313|EMBL:KYP53033.1, ECO:0000313|Proteomes:UP000075243};
RN [1] {ECO:0000313|EMBL:KYP53033.1, ECO:0000313|Proteomes:UP000075243}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Asha {ECO:0000313|Proteomes:UP000075243};
RX PubMed=22057054; DOI=10.1038/nbt.2022;
RA Varshney R.K., Chen W., Li Y., Bharti A.K., Saxena R.K., Schlueter J.A.,
RA Donoghue M.T., Azam S., Fan G., Whaley A.M., Farmer A.D., Sheridan J.,
RA Iwata A., Tuteja R., Penmetsa R.V., Wu W., Upadhyaya H.D., Yang S.P.,
RA Shah T., Saxena K.B., Michael T., McCombie W.R., Yang B., Zhang G.,
RA Yang H., Wang J., Spillane C., Cook D.R., May G.D., Xu X., Jackson S.A.;
RT "Draft genome sequence of pigeonpea (Cajanus cajan), an orphan legume crop
RT of resource-poor farmers.";
RL Nat. Biotechnol. 30:83-89(2012).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=oxaloacetate + phosphate = hydrogencarbonate +
CC phosphoenolpyruvate; Xref=Rhea:RHEA:28370, ChEBI:CHEBI:16452,
CC ChEBI:CHEBI:17544, ChEBI:CHEBI:43474, ChEBI:CHEBI:58702; EC=4.1.1.31;
CC Evidence={ECO:0000256|ARBA:ARBA00001071};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946};
CC -!- SUBUNIT: Homotetramer. {ECO:0000256|ARBA:ARBA00011881}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC -!- SIMILARITY: Belongs to the PEPCase type 1 family.
CC {ECO:0000256|ARBA:ARBA00008346}.
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DR EMBL; KQ483417; KYP53033.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A151SE33; -.
DR STRING; 3821.A0A151SE33; -.
DR OMA; KHIRISH; -.
DR Proteomes; UP000075243; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008964; F:phosphoenolpyruvate carboxylase activity; IEA:UniProtKB-EC.
DR GO; GO:0015977; P:carbon fixation; IEA:UniProtKB-KW.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro.
DR Gene3D; 1.20.1440.90; Phosphoenolpyruvate/pyruvate domain; 2.
DR HAMAP; MF_00595; PEPcase_type1; 1.
DR InterPro; IPR021135; PEP_COase.
DR InterPro; IPR022805; PEP_COase_bac/pln-type.
DR InterPro; IPR018129; PEP_COase_Lys_AS.
DR InterPro; IPR033129; PEPCASE_His_AS.
DR InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR PANTHER; PTHR30523; PHOSPHOENOLPYRUVATE CARBOXYLASE; 1.
DR PANTHER; PTHR30523:SF6; PHOSPHOENOLPYRUVATE CARBOXYLASE; 1.
DR Pfam; PF00311; PEPcase; 2.
DR PRINTS; PR00150; PEPCARBXLASE.
DR SUPFAM; SSF51621; Phosphoenolpyruvate/pyruvate domain; 1.
DR PROSITE; PS00781; PEPCASE_1; 1.
DR PROSITE; PS00393; PEPCASE_2; 1.
PE 3: Inferred from homology;
KW Carbon dioxide fixation {ECO:0000256|ARBA:ARBA00023300};
KW Lyase {ECO:0000256|ARBA:ARBA00023239};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000075243}.
FT REGION 360..393
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 407..427
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 154
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10111"
FT ACT_SITE 708
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10112"
SQ SEQUENCE 968 AA; 108329 MW; 876353F60FA56081 CRC64;
MTDITDDIAE EISFQSFDDD CRLLGNLLND ILQREVGTNL VDKIERIRVL AQSGCNMRQA
GIVNMAELLE KQLASELSEM TLEEAFTLGR AFSHYLTLMG IAETHHRVRK GGNVAQIAKS
CDDIFNQLVQ GGVPPDELYD TVCKQEVEIV LTAHPTQINR RTLQFKHIRI AHLLDYNDRP
DLSVEDREMV IEDLVREITS IWQTDELRRQ KPTPVDEARA GFNIVDQSLW KAVPHYLRRV
SNALKKHTGK PLPLTCTPIK FGSWMGGDRD GNPNVTAKVT KDVSLLSRWM AIDLYIREVD
SLRFELSMNQ CSDRLSRLAH EILEANHEKH RRENWNQSVN RSHALPTQLP ARAHLPSFAE
KGESQHPRLD IPGPDYMQSN HNKDGGVSLS STTLKNVSPN IRLSVTNSAN SSASSTANSS
STFNSGQKKL YAEPQTGKST FQKLLEPMLP QLPGIAPYRI VLGNVKDKLE KSRRRLELLL
EDVACDYDPL DYFETSDQLL EPLLLCYESL QSCGSGVLAD GRLADLIRRV ATFGMVLMKL
DLRQESGRHA ETLDAITRYL DMGTYSEWDE EKKLDFLTRE LKGKRPLVPV SIEVPSDVKE
VLDTFRIAAE LGSDSLGAYV ISMASNASDV LAVELLQKDA RLAAIGELGK ACPGGTLRVV
PLFETVKDLR GAGSVIRKLL SIDWYREHII KNHNGHQEVM VGYSDSGKDA GRFTAAWELY
KAQEDVVAAC NDYGIKVTLF HGRGGSIGRG GGPTYLAIQS QPPGSVMGTL RSTEQGEMVE
AKFGLPQIAV RQLEIYTTAV LLATLRPPLP PREENWRNVM EEISNISCQS YRSVVYENPE
FLSYFHEATP EAELGFLNIG SRPTRRKASR GIGHLRAIPW LFAWTQTRFV LPAWLGVGAG
LKGACEKGHT EELKAMYKEW PFFQSTIDLI EMVLEILKRL RRDDDNLKIR DALLITINGI
AAGMKNTG
//
