ID A0A162KMS4_9BACL Unreviewed; 854 AA.
AC A0A162KMS4;
DT 06-JUL-2016, integrated into UniProtKB/TrEMBL.
DT 06-JUL-2016, sequence version 1.
DT 27-MAR-2024, entry version 27.
DE RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900};
DE EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900};
GN ORFNames=PNBC_20740 {ECO:0000313|EMBL:OAB70993.1};
OS Paenibacillus crassostreae.
OC Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus.
OX NCBI_TaxID=1763538 {ECO:0000313|EMBL:OAB70993.1, ECO:0000313|Proteomes:UP000077134};
RN [1] {ECO:0000313|EMBL:OAB70993.1, ECO:0000313|Proteomes:UP000077134}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LPB0068 {ECO:0000313|EMBL:OAB70993.1,
RC ECO:0000313|Proteomes:UP000077134};
RA Shin S.-K., Yi H.;
RT "Paenibacillus sp. LPB0068, isolated from Crassostrea gigas.";
RL Submitted (FEB-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC Evidence={ECO:0000256|ARBA:ARBA00001279};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OAB70993.1}.
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DR EMBL; LSFN01000044; OAB70993.1; -; Genomic_DNA.
DR RefSeq; WP_068661338.1; NZ_LSFN01000044.1.
DR AlphaFoldDB; A0A162KMS4; -.
DR STRING; 1763538.LPB68_08590; -.
DR KEGG; pcx:LPB68_08590; -.
DR OrthoDB; 9814572at2; -.
DR Proteomes; UP000077134; Unassembled WGS sequence.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR GO; GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IEA:UniProtKB-EC.
DR GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1260.30; -; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR022749; D12N6_MeTrfase_N.
DR InterPro; IPR003356; DNA_methylase_A-5.
DR InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR InterPro; IPR004546; Restrct_endonuc_T1M.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR038333; T1MK-like_N_sf.
DR NCBIfam; TIGR00497; hsdM; 1.
DR PANTHER; PTHR42933:SF1; SITE-SPECIFIC DNA-METHYLTRANSFERASE (ADENINE-SPECIFIC); 1.
DR PANTHER; PTHR42933; SLR6095 PROTEIN; 1.
DR Pfam; PF12161; HsdM_N; 1.
DR Pfam; PF02384; N6_Mtase; 1.
DR PRINTS; PR00507; N12N6MTFRASE.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS00092; N6_MTASE; 1.
PE 4: Predicted;
KW Endonuclease {ECO:0000313|EMBL:OAB70993.1};
KW Hydrolase {ECO:0000313|EMBL:OAB70993.1};
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603};
KW Nuclease {ECO:0000313|EMBL:OAB70993.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000077134};
KW Restriction system {ECO:0000256|ARBA:ARBA00022747};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 7..165
FT /note="N6 adenine-specific DNA methyltransferase N-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF12161"
FT DOMAIN 176..488
FT /note="DNA methylase adenine-specific"
FT /evidence="ECO:0000259|Pfam:PF02384"
SQ SEQUENCE 854 AA; 96298 MW; 03EC406DBBCCF004 CRC64;
MITSEEIKRR LWDGANELRG SMDASRYKDY MLGLMFYKFL SDKTLEEFKI SSGLGQITES
ELVEEYVKAK VEYGEELDRM LQMALGYYVA PEYLYQSWLK DINSGNFEVQ KVTDSLNNFE
RTIAVSSDSN DFKGLFSSST LDLTDTALGS NLNERSKNIK SLILLFADLN MVALQKGDVL
GDAYEYLIGQ FAMESGKKAG EFYTPHQVSE VMAQIVAKMS DVKTIYDPTV GSGSLLLTVK
KHLGKEVQKD LSYYGQEKNT ATYNLTRMNL LLHGVRPEKM TIKNGDTLSH DWPEDPERPN
EGVQFDAVVM NPPYSVKGWN KAGLKISDPR FEIAGALPPD SKGDFAFLLH GLFHLGQNGT
MAIVLPHGVL FRGGAEGEIR ERLLEKNRID SIIGLPSSLF TNTGIPVAVM ILKKSRKIDE
PVLIIDASRS FIKVGKQNVL QEKDIAKIVD TYVERREEKG YSCLAKRKDI LENQYNMNIP
RYVEAIDEEI PQDVDAHLLG GIPQQNIEDL KVLQSTVPDI LDQSLKGIRN GYVELLKPVE
EISKEILSDA RIVAKSKEIE SKAKSYVYKY WDILRVVDNN SNLNQLMDDM LVEIKTFLAE
FKYIDVYNGY QIVAEIWKNS LVHDTEIIAL SDFYTAGRTR EPNMVTKGSG DKKREEQDGW
VGIIVPNELI AKRLYSDELE RIEKKKMRIL GIENELSDLV EAAKVEDSDE ADALGGILNE
AGEAFENKSV KAELKKASKG TVEYKLLKNV EELFTDKSTL SKAVKADEKA LKEAAQERIL
TLTNEEIDIL MYEKWFGNTV ETIVGLAELP LNAELNTLQL LHDRYVETLS VLDEESRDLE
AAFVALKSEL VVRS
//
