ID A0A163VIX5_9BACT Unreviewed; 213 AA.
AC A0A163VIX5; A0A163VC56;
DT 06-JUL-2016, integrated into UniProtKB/TrEMBL.
DT 06-JUL-2016, sequence version 1.
DT 27-MAR-2024, entry version 26.
DE RecName: Full=Thymidylate kinase {ECO:0000256|HAMAP-Rule:MF_00165};
DE EC=2.7.4.9 {ECO:0000256|HAMAP-Rule:MF_00165};
DE AltName: Full=dTMP kinase {ECO:0000256|HAMAP-Rule:MF_00165};
GN Name=tmk {ECO:0000256|HAMAP-Rule:MF_00165,
GN ECO:0000313|EMBL:NLI02623.1};
GN ORFNames=AO394_05850 {ECO:0000313|EMBL:KZD17300.1}, GX473_04760
GN {ECO:0000313|EMBL:NLI02623.1};
OS Candidatus Fermentibacter daniensis.
OC Bacteria; Candidatus Fermentibacteria; Candidatus Fermentibacteria (class);
OC Candidatus Fermentibacterales; Candidatus Fermentibacteraceae;
OC Fermentibacter.
OX NCBI_TaxID=1729713 {ECO:0000313|EMBL:KZD17300.1, ECO:0000313|Proteomes:UP000076793};
RN [1] {ECO:0000313|EMBL:KZD17300.1, ECO:0000313|Proteomes:UP000076793}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Dam_1 {ECO:0000313|EMBL:KZD17300.1};
RX PubMed=27058503;
RA Kirkegaard R.H., Dueholm M.S., McIlroy S.J., Nierychlo M., Karst S.M.,
RA Albertsen M., Nielsen P.H.;
RT "Genomic insights into members of the candidate phylum Hyd24-12 common in
RT mesophilic anaerobic digesters.";
RL ISME J. 0:0-0(2016).
RN [2] {ECO:0000313|EMBL:NLI02623.1, ECO:0000313|Proteomes:UP000567630}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AS4DglBPLU_32 {ECO:0000313|EMBL:NLI02623.1};
RX PubMed=32123542;
RA Campanaro S., Treu L., Rodriguez-R L.M., Kovalovszki A., Ziels R.M.,
RA Maus I., Zhu X., Kougias P.G., Basile A., Luo G., Schluter A.,
RA Konstantinidis K.T., Angelidaki I.;
RT "New insights from the biogas microbiome by comprehensive genome-resolved
RT metagenomics of nearly 1600 species originating from multiple anaerobic
RT digesters.";
RL Biotechnol. Biofuels 13:25-20(2020).
CC -!- FUNCTION: Phosphorylation of dTMP to form dTDP in both de novo and
CC salvage pathways of dTTP synthesis. {ECO:0000256|HAMAP-Rule:MF_00165}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + dTMP = ADP + dTDP; Xref=Rhea:RHEA:13517,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:58369, ChEBI:CHEBI:63528,
CC ChEBI:CHEBI:456216; EC=2.7.4.9;
CC Evidence={ECO:0000256|ARBA:ARBA00001008, ECO:0000256|HAMAP-
CC Rule:MF_00165};
CC -!- SIMILARITY: Belongs to the thymidylate kinase family.
CC {ECO:0000256|ARBA:ARBA00009776, ECO:0000256|HAMAP-Rule:MF_00165}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KZD17300.1}.
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DR EMBL; LKHB01000194; KZD17300.1; -; Genomic_DNA.
DR EMBL; JAAYWI010000035; NLI02623.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A163VIX5; -.
DR STRING; 1729713.AO395_00050; -.
DR Proteomes; UP000076793; Unassembled WGS sequence.
DR Proteomes; UP000567630; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004798; F:thymidylate kinase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006233; P:dTDP biosynthetic process; IEA:InterPro.
DR GO; GO:0006235; P:dTTP biosynthetic process; IEA:UniProtKB-UniRule.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR CDD; cd01672; TMPK; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR HAMAP; MF_00165; Thymidylate_kinase; 1.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR039430; Thymidylate_kin-like_dom.
DR InterPro; IPR018094; Thymidylate_kinase.
DR NCBIfam; TIGR00041; DTMP_kinase; 1.
DR PANTHER; PTHR10344; THYMIDYLATE KINASE; 1.
DR PANTHER; PTHR10344:SF4; THYMIDYLATE KINASE; 1.
DR Pfam; PF02223; Thymidylate_kin; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP-
KW Rule:MF_00165};
KW Kinase {ECO:0000256|HAMAP-Rule:MF_00165, ECO:0000313|EMBL:NLI02623.1};
KW Nucleotide biosynthesis {ECO:0000256|HAMAP-Rule:MF_00165};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_00165};
KW Transferase {ECO:0000256|HAMAP-Rule:MF_00165, ECO:0000313|EMBL:NLI02623.1}.
FT DOMAIN 11..191
FT /note="Thymidylate kinase-like"
FT /evidence="ECO:0000259|Pfam:PF02223"
FT BINDING 13..20
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00165"
SQ SEQUENCE 213 AA; 23018 MW; 3FC89380F3414D18 CRC64;
MRRPGGLFVT FEGVEGAGKT TRSAALAGSL RSIGFEVVHT REPGGPPLAE AIRRLLLDPS
LEIPTAAELM LYLASRASNV EHIVRPALAA GGVVICERFN DATEAYQAAG RGLDREFVES
ACSFATGGLE PDLTVLLDLD PAEGMARLDS RPGGPDRIER EDLAFHARVR EEYLSLARRH
RRFRVLDASL PGCEIDGIVL GLVEEALRVK EST
//
