ID A0A164KJQ7_9NOCA Unreviewed; 810 AA.
AC A0A164KJQ7;
DT 06-JUL-2016, integrated into UniProtKB/TrEMBL.
DT 06-JUL-2016, sequence version 1.
DT 27-MAR-2024, entry version 36.
DE RecName: Full=ATP-dependent DNA helicase {ECO:0000256|RuleBase:RU364053};
DE EC=5.6.2.4 {ECO:0000256|RuleBase:RU364053};
GN ORFNames=A2J03_07155 {ECO:0000313|EMBL:KZF03728.1};
OS Rhodococcus sp. EPR-157.
OC Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales; Nocardiaceae;
OC Rhodococcus.
OX NCBI_TaxID=1813677 {ECO:0000313|EMBL:KZF03728.1, ECO:0000313|Proteomes:UP000077673};
RN [1] {ECO:0000313|EMBL:KZF03728.1, ECO:0000313|Proteomes:UP000077673}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=EPR-157 {ECO:0000313|EMBL:KZF03728.1,
RC ECO:0000313|Proteomes:UP000077673};
RA Ploux O.;
RL Submitted (MAR-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618,
CC ECO:0000256|RuleBase:RU364053};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by
CC translocating in the 3'-5' direction.; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034617};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922, ECO:0000256|RuleBase:RU364053}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KZF03728.1}.
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DR EMBL; LVCV01000425; KZF03728.1; -; Genomic_DNA.
DR RefSeq; WP_068377424.1; NZ_LVCV01000425.1.
DR AlphaFoldDB; A0A164KJQ7; -.
DR OrthoDB; 9806690at2; -.
DR Proteomes; UP000077673; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0006268; P:DNA unwinding involved in DNA replication; IEA:InterPro.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR CDD; cd18807; SF1_C_UvrD; 2.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR005751; ATP-dep_DNA_helicase_PcrA.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR NCBIfam; TIGR01073; pcrA; 1.
DR PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF21196; PcrA_UvrD_tudor; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; DNA-binding {ECO:0000256|RuleBase:RU364053};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Reference proteome {ECO:0000313|Proteomes:UP000077673}.
FT DOMAIN 21..316
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 317..624
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT REGION 730..750
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 42..49
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 810 AA; 88552 MW; 3F558BBC12688923 CRC64;
MNTNLTAVPA PGKSKSEALL EGLNPQQREA VMHSGSPLLI VAGAGSGKTA VLTRRIAYLL
AERGVMPGQV LAITFTNKAA AEMRERVAGL VGPRANSMWV STFHSSCVRI LRNQASLLPG
LNSNFSIYDA DDSRRLLTMI SKDLQIDTKK FSARLLSTAI SNLKNELVGP EDAAADADKQ
MVELPKLVAQ VYGIYQQRLR SANAMDFDDL IGETVGLLQA FPQVAEYYRR RFRHVLVDEY
QDTNHAQYML VRELVGREDS DGALEGTIAP GELCVVGDAD QSIYAFRGAT IRNIEEFERD
YPDARTILLE QNYRSTQNIL SAANSVISRN TGRRDKRLWT DSGEGELIVG YVADNEHDEA
SFVASEIDRL VDNHDVRYDE VAVFYRTNNS SRALEEIFIR LGLPYKVVGG VRFYERKEVR
DMVAYLRVLS NEDDTVSMRR ILNTPRRGIG DRAEACVAVH AEQRDISFSA ALHDAAAGKV
ALLNSRSQKL IGQFLELLDE LRSVLNSTDD DGNDIADIGD VVEAVLDRTG YRAELEASSD
PQDGARLDNL NELVSVAREF TSEARNLAAV EAEADVEVER EEGSPAPGSL AAFLERVSLV
ADTDQIPDSG TGVVTLMTLH TAKGLEFPVV FVTGWEDGQF PHMRALGDPN ELSEERRLAY
VGITRARHRL YLTRAIMRSA WGQPINNPES RFLQEIPQSL IEWKREDPGI GGRPRSGGFG
GGGFGGGGGF GGGGFGERRG QQSTPSFGSA RARNNALVLA VGDRVNHDKY GLGTVLESTG
TGTSAMVLVD FGTSGRVKMM LIGGVPMTKL
//
