ID A0A176U2B9_9FIRM Unreviewed; 646 AA.
AC A0A176U2B9;
DT 07-SEP-2016, integrated into UniProtKB/TrEMBL.
DT 07-SEP-2016, sequence version 1.
DT 24-JAN-2024, entry version 18.
DE SubName: Full=Glycosyl hydrolase family 35 {ECO:0000313|EMBL:OAD86444.1};
DE Flags: Fragment;
GN ORFNames=HMPREF2738_03534 {ECO:0000313|EMBL:OAD86444.1};
OS Clostridiales bacterium KLE1615.
OC Bacteria; Bacillota; Clostridia; Eubacteriales.
OX NCBI_TaxID=1715004 {ECO:0000313|EMBL:OAD86444.1, ECO:0000313|Proteomes:UP000077212};
RN [1] {ECO:0000313|EMBL:OAD86444.1, ECO:0000313|Proteomes:UP000077212}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=KLE1615 {ECO:0000313|EMBL:OAD86444.1,
RC ECO:0000313|Proteomes:UP000077212};
RA Wen L., He K., Yang H.;
RL Submitted (FEB-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 35 family.
CC {ECO:0000256|ARBA:ARBA00009809, ECO:0000256|RuleBase:RU003679}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OAD86444.1}.
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DR EMBL; LXPQ01000257; OAD86444.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A176U2B9; -.
DR PATRIC; fig|1715004.3.peg.3525; -.
DR Proteomes; UP000077212; Unassembled WGS sequence.
DR GO; GO:0004565; F:beta-galactosidase activity; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR Gene3D; 2.60.120.260; Galactose-binding domain-like; 2.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR InterPro; IPR026283; B-gal_1-like.
DR InterPro; IPR048912; BetaGal1-like_ABD1.
DR InterPro; IPR048913; BetaGal_gal-bd.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR031330; Gly_Hdrlase_35_cat.
DR InterPro; IPR001944; Glycoside_Hdrlase_35.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR23421:SF165; BETA-GALACTOSIDASE; 1.
DR PANTHER; PTHR23421; BETA-GALACTOSIDASE RELATED; 1.
DR Pfam; PF21317; BetaGal_ABD_1; 1.
DR Pfam; PF21467; BetaGal_gal-bd; 1.
DR Pfam; PF01301; Glyco_hydro_35; 1.
DR PIRSF; PIRSF006336; B-gal; 1.
DR PRINTS; PR00742; GLHYDRLASE35.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF49785; Galactose-binding domain-like; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000313|EMBL:OAD86444.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000077212}.
FT DOMAIN 30..344
FT /note="Glycoside hydrolase 35 catalytic"
FT /evidence="ECO:0000259|Pfam:PF01301"
FT DOMAIN 407..519
FT /note="Beta-galactosidase 1-like first all-beta"
FT /evidence="ECO:0000259|Pfam:PF21317"
FT DOMAIN 563..622
FT /note="Beta-galactosidase galactose-binding"
FT /evidence="ECO:0000259|Pfam:PF21467"
FT ACT_SITE 178
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR006336-1"
FT ACT_SITE 254
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR006336-1"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:OAD86444.1"
SQ SEQUENCE 646 AA; 72988 MW; C9C9CBBE52104A4C CRC64;
AAFGVSQTPN LHEPKGEIPS MNRLRFEGSQ FYLNNQPFRI LSGTIHYFRV VPDYWEDRLL
KLKQCGFNTV ETYTCWNLHE PREGEFDFSG ILDLARFLET ASRLGLYVIL RPGPYICAEW
DMGGLPSWLL TYPHIHLRCH DELFLSKVRR YYKKLFYVIR PYLGCNGGCI IAMQVENEYG
SYGDDHTYMQ DILSIYEEEN LDCLLFTSDG PQYFMLNSGT LPNLLSAVNF GSNPKDNFAL
LRKFKSDQPL FCCEFWNGWF DHWYEEHHVR GADDTAAVLE EMLDMGASVN LYMFHGGTNF
GFTNGANFND VYQPTVTSYD YNCPLSESGD ITPKYLAIQK AVKRFWERHP GEVGPSASFA
DAISSIGNES DSVKSPSRLS KTVNLTQAAY LFAQPSLLGE GIFDSHPLTM ELLGQDFGFV
LYQTTLTGPF ETLPLTIDGL HDRALIYLDD KLVGIKERTG QRDDEVMVGL DAGQSCTLSI
LVENMGRINY GPKLLDEKGI VRGVRIGSMN HFGWIMYSIR CNDFAKVNWS SISEVLTQST
IDSVECPHPD CTSSEHSIDN FGPVLLRGFF ETDMPCDTFV RPKGFEKGII AVNGFLLGRY
YNSAGPQKTL YLPGPLLKKG KNEFIILELE HVTTPTLLLE AEPDLG
//
