ID A0A183BTT2_GLOPA Unreviewed; 1696 AA.
AC A0A183BTT2;
DT 07-SEP-2016, integrated into UniProtKB/TrEMBL.
DT 07-SEP-2016, sequence version 1.
DT 24-JAN-2024, entry version 32.
DE RecName: Full=polynucleotide adenylyltransferase {ECO:0000256|ARBA:ARBA00012388};
DE EC=2.7.7.19 {ECO:0000256|ARBA:ARBA00012388};
OS Globodera pallida (Potato cyst nematode).
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Tylenchina; Tylenchomorpha; Tylenchoidea; Heteroderidae; Heteroderinae;
OC Globodera.
OX NCBI_TaxID=36090 {ECO:0000313|Proteomes:UP000050741, ECO:0000313|WBParaSite:GPLIN_000401800};
RN [1] {ECO:0000313|Proteomes:UP000050741}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Lindley {ECO:0000313|Proteomes:UP000050741};
RA Aslett M.;
RL Submitted (DEC-2013) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Proteomes:UP000050741}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Lindley {ECO:0000313|Proteomes:UP000050741};
RA Cotton J.A., Lilley C.J., Jones L.M., Kikuchi T., Reid A.J., Thorpe P.,
RA Tsai I.J., Beasley H., Blok V., Cock P.J.A., Van den Akker S.E.,
RA Holroyd N., Hunt M., Mantelin S., Naghra H., Pain A., Palomares-Rius J.E.,
RA Zarowiecki M., Berriman M., Jones J.T., Urwin P.E.;
RT "The genome and life-stage specific transcriptomes of Globodera pallida
RT elucidate key aspects of plant parasitism by a cyst nematode.";
RL Submitted (MAY-2014) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|WBParaSite:GPLIN_000401800}
RP IDENTIFICATION.
RG WormBaseParasite;
RL Submitted (JUN-2016) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + RNA(n) = diphosphate + RNA(n)-3'-adenine ribonucleotide;
CC Xref=Rhea:RHEA:11332, Rhea:RHEA-COMP:14527, Rhea:RHEA-COMP:17347,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:140395,
CC ChEBI:CHEBI:173115; EC=2.7.7.19;
CC Evidence={ECO:0000256|ARBA:ARBA00024620};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946};
CC -!- COFACTOR:
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC Evidence={ECO:0000256|ARBA:ARBA00001936};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123}.
CC -!- SIMILARITY: Belongs to the poly(A) polymerase family.
CC {ECO:0000256|ARBA:ARBA00010912}.
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DR WBParaSite; GPLIN_000401800; GPLIN_000401800; GPLIN_000401800.
DR Proteomes; UP000050741; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:1990817; F:poly(A) RNA polymerase activity; IEA:UniProtKB-EC.
DR GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR Gene3D; 1.10.1410.10; -; 1.
DR Gene3D; 3.30.460.10; Beta Polymerase, domain 2; 1.
DR InterPro; IPR043519; NT_sf.
DR InterPro; IPR007012; PolA_pol_cen_dom.
DR InterPro; IPR048840; PolA_pol_NTPase.
DR PANTHER; PTHR10682; POLY A POLYMERASE; 1.
DR PANTHER; PTHR10682:SF10; POLYNUCLEOTIDE ADENYLYLTRANSFERASE; 1.
DR Pfam; PF04928; PAP_central; 1.
DR Pfam; PF20750; PAP_NTPase; 1.
DR SUPFAM; SSF81301; Nucleotidyltransferase; 1.
DR SUPFAM; SSF81631; PAP/OAS1 substrate-binding domain; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Coiled coil {ECO:0000256|SAM:Coils}; Membrane {ECO:0000256|SAM:Phobius};
KW mRNA processing {ECO:0000256|ARBA:ARBA00022664};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Nucleus {ECO:0000256|ARBA:ARBA00023242};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 1576..1599
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 1606..1624
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 1630..1651
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 1412..1524
FT /note="Poly(A) polymerase nucleotidyltransferase"
FT /evidence="ECO:0000259|Pfam:PF20750"
FT DOMAIN 1561..1629
FT /note="Poly(A) polymerase central"
FT /evidence="ECO:0000259|Pfam:PF04928"
FT REGION 1182..1258
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 644..671
FT /evidence="ECO:0000256|SAM:Coils"
FT COMPBIAS 1182..1257
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1696 AA; 198903 MW; E8E10ABF9DCE8BF7 CRC64;
MSKKGKFQRK LECFGRSPRH GVSENEQFEA IIDRSYLEEL LEQLEIESER NQQNICFLYQ
FGVSVHQYME LFGLNNSEWT NLLRKLQIHD AIQVHMNQSV DCDDADEVAK IFLFLFGQRT
IHTIFEYNIV KFLKMYEQLR HFFCHEIEPH SLKTELEHKL LTPASIVNLE DEFPEIFRID
LMFTATLKER CRRMLMNRSD SELYSKTYTY WAMGWLIGEL RQACAQDDSM QNVLSAHAIA
VLQKRVDDEC AIALNEMMSG RQMCVLERLV SFLINLKPKD VRAFEKRIKK WTAADECSDG
KRRADTLDQF KRDSFAKTIV KFINTAFLLH AGLNSQFLQL VRISGAKTRL FKLLGNSKER
LAELKDGRGQ LLSRFYEEDV FSQNDRLRGH YVIALADDDD DWFRFSRTLA ADVVAFMRWI
KRELGEQRLG AVYDKIRIHI DEHLYDPLFD KNAEELKKSE KFLKKMFFET KNLLAMASAK
GTKEMFFNKW KKIVEIGTKT NLSEEQLQEE QFWMAQLHFG EFIGLMEEAV DRHDALIYEY
LKKVTGNATA PSAVAGDENQ QKLITCPPKS VSVKMLRHID KENNVAFLHF LSAQCMEYLS
NHFEQIRTFK DGGHILKVQM YAILWYLSEV GAKLVKRADE LKPLGNDQNR KQKLHLEVAK
LRRNLAQLTD ITKNNGQEEL VVLKTLELRL FKMYKFVERI AVNKQFMENS SGWHQIIQQE
TNLEMLNNVP SDVDEDEMPT IYMELFEPEQ LFKITFFFIY DLLVRFTELQ KFFCDLSGVE
QREKVQARLE YAFGEQHLHT FEELVPEIMQ FDMPSPLTAA ISFKYLLCEK VGTDQFQIAE
ARLSLPRLLN EKKGWEIRTL KFDQCARKSV QQRVLQQQRK EMLPIHALFL SLILEKNAFA
FNDFVKDNRG NYHSKLIEPE MFHGERLRQL YLDALFGDDI GTRVVGKLSV FIAIFVRWMD
AELSDHPNIT IGLRQVGNMV WKNKTRPFLE DIFDELVDDS LDELEEKSYL LKELVKSLKQ
FVEEKRDLRD LDKLDMRMAS KGKQKSKLRT KTGKNMLVPM KSNNLAEEWQ QMMAEAWRKA
EEMRKAGTRE KELGEEQEEQ QLNLWLQQTQ WATIIELLED DNGRELFRNG FALCPLSEER
LTDFFSLEMA EEILELLNME NNVDIKERLK VMKQKYKEEK KEVGKANEKE QKETADRNME
EMIREEEERM RELKARIEKM QEEKRREKAE QRDRDKKEKE AKEERTKNNR KENTEFQIES
GSDTNLQVEL APVEIADFGS DDFLEGKYFD FFKNPSKSQR ELLDIGVFVN EINVAKSNVL
TRFAEHIENW RNLLAQQALD KKMFDQFSSH ITNKKLQLEN MHGMHEYMQQ IYFQDSEDKQ
KDKMLDHFLE ENGYMRKELP LNVMNEVKAT ISKWSKGEAR LIVTGSQLLG TQTPGSDIDT
ICIVPQKLTG INSYQRSCED ESLYCQFCRN SKVEEMTKIP SAYIQMLQLK LSGIKFDISF
VLVPGTEYLP DDPLDVDDVE LMMEVLATQH RPPQAMLRSL SSYLTNQGIM TLLNLKNREK
FRTLSLALKI WAKNNYIYGS IFGFFNGISL SIMATKIILL YPNGSVLFLL EKLLFVFTIW
NWPMPVKYRH FVIISCIVGI AQHIVQFCLF VERKIRLQLA HFGKIMDKTV EYGHVMPTKE
LAIQNKCPAT LXYSLS
//
