ID A0Q254_CLONN Unreviewed; 873 AA.
AC A0Q254;
DT 09-JAN-2007, integrated into UniProtKB/TrEMBL.
DT 09-JAN-2007, sequence version 1.
DT 27-MAR-2024, entry version 104.
DE SubName: Full=CRISPR-associated helicase Cas3 {ECO:0000313|EMBL:ABK62334.1};
GN OrderedLocusNames=NT01CX_0202 {ECO:0000313|EMBL:ABK62334.1};
OS Clostridium novyi (strain NT).
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Clostridiaceae;
OC Clostridium.
OX NCBI_TaxID=386415 {ECO:0000313|EMBL:ABK62334.1, ECO:0000313|Proteomes:UP000008220};
RN [1] {ECO:0000313|EMBL:ABK62334.1, ECO:0000313|Proteomes:UP000008220}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NT {ECO:0000313|EMBL:ABK62334.1,
RC ECO:0000313|Proteomes:UP000008220};
RX PubMed=17115055; DOI=10.1038/nbt1256;
RA Bettegowda C., Huang X., Lin J., Cheong I., Kohli M., Szabo S.A., Zhang X.,
RA Diaz L.A. Jr., Velculescu V.E., Parmigiani G., Kinzler K.W., Vogelstein B.,
RA Zhou S.;
RT "The genome and transcriptomes of the anti-tumor agent Clostridium novyi-
RT NT.";
RL Nat. Biotechnol. 24:1573-1580(2006).
CC -!- SIMILARITY: In the N-terminal section; belongs to the CRISPR-associated
CC nuclease Cas3-HD family. {ECO:0000256|ARBA:ARBA00006847}.
CC -!- SIMILARITY: In the central section; belongs to the CRISPR-associated
CC helicase Cas3 family. {ECO:0000256|ARBA:ARBA00009046}.
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DR EMBL; CP000382; ABK62334.1; -; Genomic_DNA.
DR RefSeq; WP_011722694.1; NC_008593.1.
DR AlphaFoldDB; A0Q254; -.
DR STRING; 386415.NT01CX_0202; -.
DR KEGG; cno:NT01CX_0202; -.
DR PATRIC; fig|386415.7.peg.1737; -.
DR eggNOG; COG1203; Bacteria.
DR HOGENOM; CLU_010123_1_1_9; -.
DR Proteomes; UP000008220; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR CDD; cd09641; Cas3''_I; 1.
DR Gene3D; 1.10.3210.30; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR006483; CRISPR-assoc_Cas3_HD.
DR InterPro; IPR038257; CRISPR-assoc_Cas3_HD_sf.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR006474; Helicase_Cas3_CRISPR-ass_core.
DR InterPro; IPR027417; P-loop_NTPase.
DR NCBIfam; TIGR01587; cas3_core; 1.
DR NCBIfam; TIGR01596; cas3_HD; 1.
DR PANTHER; PTHR47962:SF5; ATP-DEPENDENT HELICASE LHR-RELATED; 1.
DR PANTHER; PTHR47962; ATP-DEPENDENT HELICASE LHR-RELATED-RELATED; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51643; HD_CAS3; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
PE 3: Inferred from homology;
KW Antiviral defense {ECO:0000256|ARBA:ARBA00023118};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Reference proteome {ECO:0000313|Proteomes:UP000008220}.
FT DOMAIN 28..257
FT /note="HD Cas3-type"
FT /evidence="ECO:0000259|PROSITE:PS51643"
FT DOMAIN 328..523
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
SQ SEQUENCE 873 AA; 103429 MW; 89B60A52DDA229D2 CRC64;
MYFDKSKKFN IEMYINNSDK IYAHTMDDNK KVETLKEHLE RSIKYFYKLV ENKNLDNIFL
KFEAKLCKEF SDKEKSLFRE MIVNTIYMHD LGKININFQT IKMKNKYFKD KKDIECSNSN
HSCLSSLIYM DYYHKKIKSD SELINKENKK KLRLFMVLNA YVISKHHTGL DKLDDFRAKL
TQDDGEGQRL CSSELCIFED MYNKVIEFTT KDNILGKLFK LAEKTLKVYE EEEKNISVLF
YIYVRLMLST LLMCDYYSTS EFENCSEVKG FNDIEDIHDF YDTFNSTNIN KDTREYEKSK
YGLKEDFSNV DDINILRKEL FLDAEKELLR NLDKNIFYLE APTGSGKSNV AFNLTFKLIE
KQKDLKKLIY VYPFNTLVEQ NTRTIEKIFN NQEYILDSMA VINSITPIKV KNKDSDENTK
DYNKSLLDRQ FLNYPMILTT HVSLFNYFFG ISKEDLFPLV KLCNSVIVLD EIQSYKNYIW
KEIITFLNYY SELLNIKFII MSATLPKLDV LIDGESNSVN LILNREKYFK NRLFKDRVKL
DYSLIDLELD DKDEKLEEIL NQVIEQANSS NKNILIEFIN KSTAVDFYEM LKDYKYEYGL
GNDDKRDIEL ITGDDNSIDR NIIINKINKE KNIILVATQV IEAGVDIDMD IGYKDISMID
CDEQFLGRIN RSCLKESGIV YFFNIDSAGG IYKNDYRKQK QLTLISEKFD TKKWLENKEF
DKFYEYVLEI INKNGNDEYK EGSMVNFIEN IVSKLNFMDV SERMKLIDDD KMESSVFLSR
EIQLEGGEVL KGKEVWNSYK ELLKDNEMDY AERKVKLSKV VSYMNYFIYK VKKCNFNYTE
YIGEIFYIED GEEFFTNGKF DRKKFEGINL DIC
//
