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Database: UniProt
Entry: A0R643
LinkDB: A0R643
Original site: A0R643 
ID   PHEA_MYCS2              Reviewed;         310 AA.
AC   A0R643; I7GFR7;
DT   01-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2007, sequence version 1.
DT   09-JUL-2014, entry version 51.
DE   RecName: Full=Prephenate dehydratase;
DE            Short=PDT;
DE            EC=4.2.1.51;
GN   Name=pheA; OrderedLocusNames=MSMEG_6418, MSMEI_6250;
OS   Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155).
OC   Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales;
OC   Corynebacterineae; Mycobacteriaceae; Mycobacterium.
OX   NCBI_TaxID=246196;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RA   Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C.,
RA   Fraser C.M.;
RL   Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=17295914; DOI=10.1186/gb-2007-8-2-r20;
RA   Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C.,
RA   Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.;
RT   "Interrupted coding sequences in Mycobacterium smegmatis: authentic
RT   mutations or sequencing errors?";
RL   Genome Biol. 8:R20.1-R20.9(2007).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=18955433; DOI=10.1101/gr.081901.108;
RA   Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M.,
RA   Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.;
RT   "Ortho-proteogenomics: multiple proteomes investigation through
RT   orthology and a new MS-based protocol.";
RL   Genome Res. 19:128-135(2009).
CC   -!- CATALYTIC ACTIVITY: Prephenate = phenylpyruvate + H(2)O + CO(2).
CC   -!- PATHWAY: Amino-acid biosynthesis; L-phenylalanine biosynthesis;
CC       phenylpyruvate from prephenate: step 1/1.
CC   -!- SUBUNIT: Homodimer (By similarity).
CC   -!- SIMILARITY: Contains 1 ACT domain.
CC   -!- SIMILARITY: Contains 1 prephenate dehydratase domain.
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DR   EMBL; CP000480; ABK72631.1; -; Genomic_DNA.
DR   EMBL; CP001663; AFP42676.1; -; Genomic_DNA.
DR   RefSeq; YP_006570971.1; NC_018289.1.
DR   RefSeq; YP_890631.1; NC_008596.1.
DR   ProteinModelPortal; A0R643; -.
DR   STRING; 246196.MSMEG_6418; -.
DR   EnsemblBacteria; ABK72631; ABK72631; MSMEG_6418.
DR   EnsemblBacteria; AFP42676; AFP42676; MSMEI_6250.
DR   GeneID; 4534511; -.
DR   KEGG; msg:MSMEI_6250; -.
DR   KEGG; msm:MSMEG_6418; -.
DR   PATRIC; 18085063; VBIMycSme59918_6244.
DR   eggNOG; COG0077; -.
DR   HOGENOM; HOG000018970; -.
DR   KO; K04518; -.
DR   OMA; EHGDWAK; -.
DR   OrthoDB; EOG6WHNT1; -.
DR   BioCyc; MSME246196:GJ4Y-6417-MONOMER; -.
DR   UniPathway; UPA00121; UER00345.
DR   GO; GO:0016597; F:amino acid binding; IEA:InterPro.
DR   GO; GO:0004664; F:prephenate dehydratase activity; ISS:UniProtKB.
DR   GO; GO:0042803; F:protein homodimerization activity; ISS:UniProtKB.
DR   GO; GO:0033585; P:L-phenylalanine biosynthetic process from chorismate via phenylpyruvate; ISS:UniProtKB.
DR   InterPro; IPR002912; ACT_dom.
DR   InterPro; IPR001086; Preph_deHydtase.
DR   InterPro; IPR018528; Preph_deHydtase_CS.
DR   Pfam; PF01842; ACT; 1.
DR   Pfam; PF00800; PDT; 1.
DR   PROSITE; PS51671; ACT; 1.
DR   PROSITE; PS00858; PREPHENATE_DEHYDR_2; 1.
DR   PROSITE; PS51171; PREPHENATE_DEHYDR_3; 1.
PE   3: Inferred from homology;
KW   Amino-acid biosynthesis; Aromatic amino acid biosynthesis;
KW   Complete proteome; Lyase; Phenylalanine biosynthesis;
KW   Reference proteome.
FT   CHAIN         1    310       Prephenate dehydratase.
FT                                /FTId=PRO_0000382039.
FT   DOMAIN        3    190       Prephenate dehydratase.
FT   DOMAIN      204    281       ACT.
FT   SITE        183    183       Essential for activity (By similarity).
SQ   SEQUENCE   310 AA;  32464 MW;  05E0E37D5F39C0B2 CRC64;
     MPRIAYLGPE GTFTEAALLQ MVAKGMVPGP AEDAGGFTPV RTDSTPGALS AVREGRADYA
     CVPIENSIDG TVLPTLDSLA AGSPLQIYAE LTLDVAFTIV VRPGHDGPVR TVAAFPVAAA
     QVRHWLAANL RDAEVVPAHS NAAAAHDVAE GRADAGVSTR LAAERCGLDI MAADVVDEPN
     ARTRFVLVGL PGTPPPATGA DRTAVVLRLV NEPGALVSAM TEFSIRDIDL TRIESRPTRT
     ELGTYMFFLD CAGHIDDDPV AEALKALHRR CVDVRYLGSW PTESAAGAPP PRLDEATTWL
     EGLRAGSGGA
//
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