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Database: UniProt
Entry: A0RQK7
LinkDB: A0RQK7
Original site: A0RQK7 
ID   LEU1_CAMFF              Reviewed;         506 AA.
AC   A0RQK7;
DT   28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2007, sequence version 1.
DT   01-OCT-2014, entry version 56.
DE   RecName: Full=2-isopropylmalate synthase {ECO:0000255|HAMAP-Rule:MF_01025};
DE            EC=2.3.3.13 {ECO:0000255|HAMAP-Rule:MF_01025};
DE   AltName: Full=Alpha-IPM synthase {ECO:0000255|HAMAP-Rule:MF_01025};
DE   AltName: Full=Alpha-isopropylmalate synthase {ECO:0000255|HAMAP-Rule:MF_01025};
GN   Name=leuA {ECO:0000255|HAMAP-Rule:MF_01025};
GN   OrderedLocusNames=CFF8240_1344;
OS   Campylobacter fetus subsp. fetus (strain 82-40).
OC   Bacteria; Proteobacteria; Epsilonproteobacteria; Campylobacterales;
OC   Campylobacteraceae; Campylobacter.
OX   NCBI_TaxID=360106;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=82-40;
RA   Fouts D.E., Nelson K.E.;
RT   "Sequence of Campylobacter fetus subsp. fetus 82-40.";
RL   Submitted (NOV-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the condensation of the acetyl group of
CC       acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form
CC       3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate).
CC       {ECO:0000255|HAMAP-Rule:MF_01025}.
CC   -!- CATALYTIC ACTIVITY: Acetyl-CoA + 3-methyl-2-oxobutanoate + H(2)O =
CC       (2S)-2-isopropylmalate + CoA. {ECO:0000255|HAMAP-Rule:MF_01025}.
CC   -!- PATHWAY: Amino-acid biosynthesis; L-leucine biosynthesis; L-
CC       leucine from 3-methyl-2-oxobutanoate: step 1/4.
CC       {ECO:0000255|HAMAP-Rule:MF_01025}.
CC   -!- SUBUNIT: Homotetramer. {ECO:0000255|HAMAP-Rule:MF_01025}.
CC   -!- SIMILARITY: Belongs to the alpha-IPM synthase/homocitrate synthase
CC       family. LeuA type 1 subfamily. {ECO:0000255|HAMAP-Rule:MF_01025}.
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DR   EMBL; CP000487; ABK82096.1; -; Genomic_DNA.
DR   RefSeq; YP_892490.1; NC_008599.1.
DR   ProteinModelPortal; A0RQK7; -.
DR   STRING; 360106.CFF8240_1344; -.
DR   EnsemblBacteria; ABK82096; ABK82096; CFF8240_1344.
DR   GeneID; 4539073; -.
DR   KEGG; cff:CFF8240_1344; -.
DR   PATRIC; 20037206; VBICamFet25865_1308.
DR   eggNOG; COG0119; -.
DR   HOGENOM; HOG000046859; -.
DR   KO; K01649; -.
DR   OMA; EGQNALG; -.
DR   OrthoDB; EOG6CGCF3; -.
DR   BioCyc; CFET360106:GHTH-1342-MONOMER; -.
DR   UniPathway; UPA00048; UER00070.
DR   GO; GO:0003852; F:2-isopropylmalate synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009098; P:leucine biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.20.20.70; -; 1.
DR   HAMAP; MF_01025; LeuA_type1; 1.
DR   InterPro; IPR013709; 2-isopropylmalate_synth_dimer.
DR   InterPro; IPR002034; AIPM/Hcit_synth_CS.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR005671; LeuA_bact_synth.
DR   InterPro; IPR000891; PYR_CT.
DR   Pfam; PF00682; HMGL-like; 1.
DR   Pfam; PF08502; LeuA_dimer; 1.
DR   SMART; SM00917; LeuA_dimer; 1.
DR   SUPFAM; SSF110921; SSF110921; 1.
DR   TIGRFAMs; TIGR00973; leuA_bact; 1.
DR   PROSITE; PS00815; AIPM_HOMOCIT_SYNTH_1; 1.
DR   PROSITE; PS00816; AIPM_HOMOCIT_SYNTH_2; 1.
DR   PROSITE; PS50991; PYR_CT; 1.
PE   3: Inferred from homology;
KW   Amino-acid biosynthesis; Branched-chain amino acid biosynthesis;
KW   Complete proteome; Leucine biosynthesis; Reference proteome;
KW   Transferase.
FT   CHAIN         1    506       2-isopropylmalate synthase.
FT                                /FTId=PRO_1000149159.
SQ   SEQUENCE   506 AA;  54896 MW;  EA0FBEE7E49F01E2 CRC64;
     MDNNKIIVFD TTLRDGEQSP GASMNTEEKI QIALQLERLG VDVMEAGFAA ASPGDFDAIN
     QIAKQIHSIR IASLARALEK DIKAAGEAIS PAKNRRIHTF IATSPIHMEH KLKMTPDEVI
     KRAVEAVKYA KTFVDDVEFS CEDAGRSDIV FLKEICAAVV EAGARTLNLP DTVGFRMPDE
     IYNMVKSMVD FIGDRAIISV HNHNDLGLAV ANTLASIKAG ARQVECTING LGERAGNAAL
     EEIVMTIRTR SDEFAPLYTD IVTKEIYATS RLVASITGIE PQPNKAIVGK NAFAHESGIH
     QDGMLKCAQT YEIIKAEDIG AEKNSLVLGK HSGRHAFKDK LINLGFDLDD NEINEAFIKF
     KELCDKKKEI FDDDIRALVS HEIIKIPEIY SIQTLSTSSC NAGHSSAAVS IKFSDNIISD
     AALGNGTADA IFKVIDRISG ISGELKDYKV NAVSQGKDAL AKITVKVVFE GSSCATIGHG
     LDIDTMMASA KAYVSALNSY LSMKNR
//
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