ID GATB_NEOFI Reviewed; 601 AA.
AC A1D6S8;
DT 19-OCT-2011, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 1.
DT 03-APR-2013, entry version 41.
DE RecName: Full=Glutamyl-tRNA(Gln) amidotransferase subunit B, mitochondrial;
DE Short=Glu-AdT subunit B;
DE EC=6.3.5.-;
DE Flags: Precursor;
GN ORFNames=NFIA_065860;
OS Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / FGSC A1164 / NRRL
OS 181) (Aspergillus fischerianus).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Trichocomaceae; Neosartorya.
OX NCBI_TaxID=331117;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 1020 / DSM 3700 / FGSC A1164 / NRRL 181;
RX PubMed=18404212; DOI=10.1371/journal.pgen.1000046;
RA Fedorova N.D., Khaldi N., Joardar V.S., Maiti R., Amedeo P.,
RA Anderson M.J., Crabtree J., Silva J.C., Badger J.H., Albarraq A.,
RA Angiuoli S., Bussey H., Bowyer P., Cotty P.J., Dyer P.S., Egan A.,
RA Galens K., Fraser-Liggett C.M., Haas B.J., Inman J.M., Kent R.,
RA Lemieux S., Malavazi I., Orvis J., Roemer T., Ronning C.M.,
RA Sundaram J.P., Sutton G., Turner G., Venter J.C., White O.R.,
RA Whitty B.R., Youngman P., Wolfe K.H., Goldman G.H., Wortman J.R.,
RA Jiang B., Denning D.W., Nierman W.C.;
RT "Genomic islands in the pathogenic filamentous fungus Aspergillus
RT fumigatus.";
RL PLoS Genet. 4:E1000046-E1000046(2008).
CC -!- FUNCTION: Allows the formation of correctly charged Gln-tRNA(Gln)
CC through the transamidation of misacylated Glu-tRNA(Gln) in the
CC mitochondria. The reaction takes place in the presence of
CC glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln)
CC (By similarity).
CC -!- CATALYTIC ACTIVITY: ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP
CC + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate.
CC -!- SUBUNIT: Subunit of the heterotrimeric GatCAB amidotransferase
CC (AdT) complex, composed of A, B and C subunits (By similarity).
CC -!- SUBCELLULAR LOCATION: Mitochondrion (Potential).
CC -!- SIMILARITY: Belongs to the GatB/GatE family. GatB subfamily.
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DR EMBL; DS027690; EAW21422.1; -; Genomic_DNA.
DR RefSeq; XP_001263319.1; XM_001263318.1.
DR ProteinModelPortal; A1D6S8; -.
DR STRING; 36630.CADNFIAP00005676; -.
DR EnsemblFungi; CADNFIAT00005833; CADNFIAP00005676; CADNFIAG00005833.
DR GeneID; 4589934; -.
DR KEGG; nfi:NFIA_065860; -.
DR HOGENOM; HOG000223743; -.
DR KO; K02434; -.
DR OrthoDB; EOG473T0X; -.
DR GO; GO:0030956; C:glutamyl-tRNA(Gln) amidotransferase complex; IEA:HAMAP.
DR GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0050567; F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity; IEA:HAMAP.
DR GO; GO:0070681; P:glutaminyl-tRNAGln biosynthesis via transamidation; IEA:HAMAP.
DR GO; GO:0032543; P:mitochondrial translation; IEA:HAMAP.
DR HAMAP; MF_00121; GatB; 1; -.
DR InterPro; IPR004413; Apn/Gln-ADT_bsu.
DR InterPro; IPR017959; Asn/Gln-tRNA_amidoTrfase_suB/E.
DR InterPro; IPR006075; Asn/Gln-tRNA_Trfase_suB/E_cat.
DR InterPro; IPR018027; Asn/Gln_amidotransferase.
DR InterPro; IPR003789; Asn/Gln_tRNA_amidoTrfrase-rel.
DR InterPro; IPR017958; Gln-tRNA_amidoTrfase_suB_CS.
DR PANTHER; PTHR11659; PTHR11659; 1.
DR Pfam; PF02934; GatB_N; 1.
DR Pfam; PF02637; GatB_Yqey; 1.
DR SMART; SM00845; GatB_Yqey; 1.
DR SUPFAM; SSF89095; GatB_Yqey; 1.
DR TIGRFAMs; TIGR00133; gatB; 1.
DR PROSITE; PS01234; GATB; 1.
PE 3: Inferred from homology;
KW ATP-binding; Complete proteome; Ligase; Mitochondrion;
KW Nucleotide-binding; Protein biosynthesis; Transit peptide.
FT TRANSIT 1 52 Mitochondrion (Potential).
FT CHAIN 53 601 Glutamyl-tRNA(Gln) amidotransferase
FT subunit B, mitochondrial.
FT /FTId=PRO_0000413264.
SQ SEQUENCE 601 AA; 66768 MW; 70C56FC754772496 CRC64;
MLQQWLRQSP GAARFLRGSC CRGPQSGSLR HSPLPTAPHR CIRSLQTSAT ESKEHIPLRK
QLKQNAKALK AQKRQKRESE EASRQKWELT VGIEIHAQLN TETKLFSRAS TSNTDTPNSN
VALFDLAFPG SQPEFQATTL LPALRAAIAL NCDIQPVSRF DRKHYFYHDQ PAGYQITQYY
APFAKNGYVD LFPHDGIAPE DGDHVRIGIK QIQLEQDTAK SQEYPPSTQL LDFNRVSHPL
IEIITMPQIH NPATAAACVR KIQAILQSCS AVTTGMELGG LRADVNVSIR RRDEAPGTHQ
YGGIGGLGQR TEIKNLSSFK AVEDAVIAEK NRQIAVLESG GVIEGETRGW TIGSTETRKL
RGKEGEVDYR YMPDPDLPPL IISHDLVSGL RDSLPTPPDQ LIEMLAGPEY GLSIEDAKPL
IELDDGARLE YYQDVVDILR DLQQDQDAKS RAGLARMAGN WVLHELGGLC AKADLAWDAQ
RVPAETLAQI IDQLQRKRIT GATAKQVLAM VFDGDRRPVP QLLEAENLLL RPLSRDEYIA
LAEAAISQNP QMVEQIRAKN QLGKLGWFVG QMMRMGEKGR VEAQKADEIL RELILGKSDQ
P
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