GenomeNet

Database: UniProt
Entry: A1JK30
LinkDB: A1JK30
Original site: A1JK30 
ID   FADJ_YERE8              Reviewed;         745 AA.
AC   A1JK30;
DT   18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT   18-MAR-2008, sequence version 2.
DT   19-FEB-2014, entry version 56.
DE   RecName: Full=Fatty acid oxidation complex subunit alpha;
DE   Includes:
DE     RecName: Full=Enoyl-CoA hydratase/3-hydroxybutyryl-CoA epimerase;
DE              EC=4.2.1.17;
DE              EC=5.1.2.3;
DE   Includes:
DE     RecName: Full=3-hydroxyacyl-CoA dehydrogenase;
DE              EC=1.1.1.35;
GN   Name=fadJ; Synonyms=faoA; OrderedLocusNames=YE1277;
OS   Yersinia enterocolitica serotype O:8 / biotype 1B (strain NCTC 13174 /
OS   8081).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Yersinia.
OX   NCBI_TaxID=393305;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NCTC 13174 / 8081;
RX   PubMed=17173484; DOI=10.1371/journal.pgen.0020206;
RA   Thomson N.R., Howard S., Wren B.W., Holden M.T.G., Crossman L.,
RA   Challis G.L., Churcher C., Mungall K., Brooks K., Chillingworth T.,
RA   Feltwell T., Abdellah Z., Hauser H., Jagels K., Maddison M., Moule S.,
RA   Sanders M., Whitehead S., Quail M.A., Dougan G., Parkhill J.,
RA   Prentice M.B.;
RT   "The complete genome sequence and comparative genome analysis of the
RT   high pathogenicity Yersinia enterocolitica strain 8081.";
RL   PLoS Genet. 2:2039-2051(2006).
CC   -!- FUNCTION: Catalyzes the formation of a hydroxyacyl-CoA by addition
CC       of water on enoyl-CoA. Also exhibits 3-hydroxyacyl-CoA epimerase
CC       and 3-hydroxyacyl-CoA dehydrogenase activities (By similarity).
CC   -!- CATALYTIC ACTIVITY: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-
CC       CoA + H(2)O.
CC   -!- CATALYTIC ACTIVITY: (S)-3-hydroxyacyl-CoA + NAD(+) = 3-oxoacyl-CoA
CC       + NADH.
CC   -!- CATALYTIC ACTIVITY: (S)-3-hydroxybutanoyl-CoA = (R)-3-
CC       hydroxybutanoyl-CoA.
CC   -!- PATHWAY: Lipid metabolism; fatty acid beta-oxidation.
CC   -!- SUBUNIT: Heterotetramer of two alpha chains (FadJ) and two beta
CC       chains (FadI) (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- SIMILARITY: In the N-terminal section; belongs to the enoyl-CoA
CC       hydratase/isomerase family.
CC   -!- SIMILARITY: In the central section; belongs to the 3-hydroxyacyl-
CC       CoA dehydrogenase family.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAL11369.1; Type=Erroneous initiation;
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; AM286415; CAL11369.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; YP_001005598.1; NC_008800.1.
DR   ProteinModelPortal; A1JK30; -.
DR   STRING; 393305.YE1277; -.
DR   EnsemblBacteria; CAL11369; CAL11369; YE1277.
DR   GeneID; 4715636; -.
DR   KEGG; yen:YE1277; -.
DR   PATRIC; 18562028; VBIYerEnt11519_1387.
DR   eggNOG; COG1250; -.
DR   HOGENOM; HOG000261346; -.
DR   KO; K01782; -.
DR   OrthoDB; EOG6M9F0M; -.
DR   ProtClustDB; PRK11154; -.
DR   UniPathway; UPA00659; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003857; F:3-hydroxyacyl-CoA dehydrogenase activity; IEA:UniProtKB-HAMAP.
DR   GO; GO:0008692; F:3-hydroxybutyryl-CoA epimerase activity; IEA:UniProtKB-HAMAP.
DR   GO; GO:0004300; F:enoyl-CoA hydratase activity; IEA:UniProtKB-HAMAP.
DR   GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR   GO; GO:0006635; P:fatty acid beta-oxidation; IEA:UniProtKB-UniPathway.
DR   Gene3D; 1.10.1040.10; -; 2.
DR   Gene3D; 3.40.50.720; -; 1.
DR   HAMAP; MF_01617; FadJ; 1.
DR   InterPro; IPR006180; 3-OHacyl-CoA_DH_CS.
DR   InterPro; IPR006176; 3-OHacyl-CoA_DH_NAD-bd.
DR   InterPro; IPR006108; 3HC_DH_C.
DR   InterPro; IPR008927; 6-PGluconate_DH_C-like.
DR   InterPro; IPR001753; Crotonase_core_superfam.
DR   InterPro; IPR013328; DH_multihelical.
DR   InterPro; IPR012802; FadJ.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   Pfam; PF00725; 3HCDH; 2.
DR   Pfam; PF02737; 3HCDH_N; 1.
DR   Pfam; PF00378; ECH; 1.
DR   SUPFAM; SSF48179; SSF48179; 2.
DR   TIGRFAMs; TIGR02440; FadJ; 1.
DR   PROSITE; PS00067; 3HCDH; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Cytoplasm; Fatty acid metabolism; Isomerase;
KW   Lipid degradation; Lipid metabolism; Lyase; Multifunctional enzyme;
KW   NAD; Oxidoreductase.
FT   CHAIN         1    745       Fatty acid oxidation complex subunit
FT                                alpha.
FT                                /FTId=PRO_0000323532.
FT   REGION       47    209       Enoyl-CoA hydratase (By similarity).
FT   REGION      325    745       3-hydroxyacyl-CoA dehydrogenase (By
FT                                similarity).
FT   SITE        137    137       Important for catalytic activity (By
FT                                similarity).
FT   SITE        159    159       Important for catalytic activity (By
FT                                similarity).
SQ   SEQUENCE   745 AA;  79924 MW;  B31E7B68E3F51798 CRC64;
     MSQENTLNSG DETILAEAVA PAHAVFTLSV RPDNIGIITV DVVGDKVNTL KAKFAEQIAE
     ILQQAQALSQ LQGLVIISGK PDSFIAGADI TMIAACHTAQ DARILAQKGQ SILAQIAAFP
     VPVVAAIHGA CLGGGLELAL ACHSRICSQD DKTVLGLPEV QLGLLPGSGG TQRLPRLVGV
     SKALDMILTG RQVRARQALK MGLVDDVVPQ DILLDVAIQR AKAGWLDKPA LPWQERLLSG
     PLGKALLFNI VRKKTQAKTK GHYPAAERII DVVRKGLDHG GPAGYEAEAK AFGELAMTPE
     SAALRSLFFA TTSLKKESGG KAQPRAIHRV GVLGGGLMGG GIANVTATRA GLPVRIKDIN
     PTGINQALKY TWDTLGKRVR SKRMRPAERQ RQMMLISGST DYCGFGNVDI VVEAVFEDLS
     LKQQMVADIE HFAAPHTIFA SNTSSLPISD IAAQAQRPEQ VIGLHYFSPV DKMPLVEVIP
     HAKTSEETIA TTVALARKQG KTAIVVADRA GFYVNRILAP YINEAARCLL DGEPIASVDK
     ALVDFGFPVG PITLLDEVGI DVGTKIIPIL VEKLGARFAA PPSFDVILKD GRKGRKNGRG
     FYLYPAKSSG FKWKRSPVKQ VDTSVYTLLG VTPKAHLESA VIAQRCTMMM LNEAARCLDE
     SIIRNPRDGD IGAVFGIGFP PFLGGPFRYM DSLGADKVVK TLNLLAQQYG ERFEPCSLLV
     TMAGQQKRFY PPENSLDEAA ITAHN
//
DBGET integrated database retrieval system