ID DDL_POLNA Reviewed; 325 AA.
AC A1VST4;
DT 10-JUN-2008, integrated into UniProtKB/Swiss-Prot.
DT 06-FEB-2007, sequence version 1.
DT 01-MAY-2013, entry version 52.
DE RecName: Full=D-alanine--D-alanine ligase;
DE EC=6.3.2.4;
DE AltName: Full=D-Ala-D-Ala ligase;
DE AltName: Full=D-alanylalanine synthetase;
GN Name=ddl; OrderedLocusNames=Pnap_3415;
OS Polaromonas naphthalenivorans (strain CJ2).
OC Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC Comamonadaceae; Polaromonas.
OX NCBI_TaxID=365044;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CJ2;
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H.,
RA Pitluck S., Sims D.R., Brettin T., Bruce D., Han C., Tapia R.,
RA Brainard J., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N.,
RA Kim E., Madsen E.L., Richardson P.;
RT "Complete sequence of chromosome 1 of Polaromonas naphthalenivorans
RT CJ2.";
RL Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Cell wall formation (By similarity).
CC -!- CATALYTIC ACTIVITY: ATP + 2 D-alanine = ADP + phosphate + D-
CC alanyl-D-alanine.
CC -!- COFACTOR: Binds 2 magnesium or manganese ions per subunit (By
CC similarity).
CC -!- PATHWAY: Cell wall biogenesis; peptidoglycan biosynthesis.
CC -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC -!- SIMILARITY: Belongs to the D-alanine--D-alanine ligase family.
CC -!- SIMILARITY: Contains 1 ATP-grasp domain.
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DR EMBL; CP000529; ABM38712.1; -; Genomic_DNA.
DR RefSeq; YP_983633.1; NC_008781.1.
DR ProteinModelPortal; A1VST4; -.
DR SMR; A1VST4; 9-313.
DR STRING; 365044.Pnap_3415; -.
DR EnsemblBacteria; ABM38712; ABM38712; Pnap_3415.
DR GeneID; 4688839; -.
DR KEGG; pna:Pnap_3415; -.
DR PATRIC; 22951551; VBIPolNap76733_4296.
DR eggNOG; COG1181; -.
DR HOGENOM; HOG000011592; -.
DR KO; K01921; -.
DR OMA; GCRTWGR; -.
DR ProtClustDB; PRK01372; -.
DR BioCyc; PNAP365044:GJ8X-3464-MONOMER; -.
DR UniPathway; UPA00219; -.
DR GO; GO:0005618; C:cell wall; IEA:InterPro.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:HAMAP.
DR GO; GO:0008716; F:D-alanine-D-alanine ligase activity; IEA:HAMAP.
DR GO; GO:0000287; F:magnesium ion binding; IEA:HAMAP.
DR GO; GO:0030145; F:manganese ion binding; IEA:HAMAP.
DR GO; GO:0009252; P:peptidoglycan biosynthetic process; IEA:HAMAP.
DR GO; GO:0008360; P:regulation of cell shape; IEA:UniProtKB-KW.
DR Gene3D; 3.30.1490.20; -; 1.
DR Gene3D; 3.30.470.20; -; 2.
DR Gene3D; 3.40.50.20; -; 1.
DR HAMAP; MF_00047; Dala_Dala_lig; 1; -.
DR InterPro; IPR011761; ATP-grasp.
DR InterPro; IPR013815; ATP_grasp_subdomain_1.
DR InterPro; IPR013816; ATP_grasp_subdomain_2.
DR InterPro; IPR000291; D-Ala_lig_Van_CS.
DR InterPro; IPR005905; D_ala_D_ala.
DR InterPro; IPR011095; Dala_Dala_lig_C.
DR InterPro; IPR011127; Dala_Dala_lig_N.
DR InterPro; IPR016185; PreATP-grasp_dom.
DR PANTHER; PTHR23132; PTHR23132; 1.
DR Pfam; PF07478; Dala_Dala_lig_C; 1.
DR Pfam; PF01820; Dala_Dala_lig_N; 2.
DR SUPFAM; SSF52440; PreATP-grasp-like; 1.
DR TIGRFAMs; TIGR01205; D_ala_D_alaTIGR; 1.
DR PROSITE; PS50975; ATP_GRASP; 1.
DR PROSITE; PS00843; DALA_DALA_LIGASE_1; 1.
DR PROSITE; PS00844; DALA_DALA_LIGASE_2; 1.
PE 3: Inferred from homology;
KW ATP-binding; Cell shape; Cell wall biogenesis/degradation;
KW Complete proteome; Cytoplasm; Ligase; Magnesium; Manganese;
KW Metal-binding; Nucleotide-binding; Peptidoglycan synthesis.
FT CHAIN 1 325 D-alanine--D-alanine ligase.
FT /FTId=PRO_0000341150.
FT DOMAIN 107 311 ATP-grasp.
FT NP_BIND 137 192 ATP (By similarity).
FT METAL 264 264 Magnesium or manganese 1 (By similarity).
FT METAL 278 278 Magnesium or manganese 1 (By similarity).
FT METAL 278 278 Magnesium or manganese 2 (By similarity).
FT METAL 280 280 Magnesium or manganese 2 (By similarity).
SQ SEQUENCE 325 AA; 34584 MW; A75A4DD1D3D691F3 CRC64;
MSPNPSSFGK VAVLMGGRSA EREISLMSGS GVVQALRSQG IDAHAFDPAE RDLGELKTGG
FARCFIALHG RFGEDGTVQG ALELLGIPYT GSGVMASSMA IDKVMTKRLL LSESLPTPRY
VLLRRGGYSP AQVDAIVDTL GLPLIVKPAR EGSSLGLSKV TERAAMAAAV ALAEKMDADI
LCEQFISGDE VTCPVLGTGE QARALPVIRI VAPEGNYDYQ NKYFTDTTQY LVPCGLPAGE
EAAIQQLVLQ AFRTLNCRGW ARADVMIDQA TRKPYLLEIN TSPGMTGHSL VPMSARAAGI
SYEALCVEVL KTALLDHQPR DGSLP
//
