GenomeNet

Database: UniProt
Entry: A3DCH2_CLOTH
LinkDB: A3DCH2_CLOTH
Original site: A3DCH2_CLOTH 
ID   A3DCH2_CLOTH            Unreviewed;      1224 AA.
AC   A3DCH2;
DT   20-MAR-2007, integrated into UniProtKB/TrEMBL.
DT   20-MAR-2007, sequence version 1.
DT   22-NOV-2017, entry version 89.
DE   RecName: Full=Glucanase {ECO:0000256|RuleBase:RU361166};
DE            EC=3.2.1.- {ECO:0000256|RuleBase:RU361166};
DE   Flags: Precursor;
GN   OrderedLocusNames=Cthe_0413 {ECO:0000313|EMBL:ABN51651.1};
OS   Clostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 /
OS   NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium
OS   thermocellum).
OC   Bacteria; Firmicutes; Clostridia; Clostridiales; Ruminococcaceae;
OC   Ruminiclostridium.
OX   NCBI_TaxID=203119 {ECO:0000313|EMBL:ABN51651.1, ECO:0000313|Proteomes:UP000002145};
RN   [1] {ECO:0000313|Proteomes:UP000002145}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536
RC   / VPI 7372 {ECO:0000313|Proteomes:UP000002145};
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H.,
RA   Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Tapia R.,
RA   Gilna P., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N.,
RA   Mikhailova N., Wu J.H.D., Newcomb M., Richardson P.;
RT   "Complete sequence of Clostridium thermocellum ATCC 27405.";
RL   Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000213|PDB:3PDD, ECO:0000213|PDB:3PDG, ECO:0000213|PDB:3PE9}
RP   X-RAY CRYSTALLOGRAPHY (1.69 ANGSTROMS) OF 915-1003 IN COMPLEX WITH
RP   CALCIUM.
RX   PubMed=22349231; DOI=10.1107/S0907444912001680;
RA   Brunecky R., Alahuhta M., Bomble Y.J., Xu Q., Baker J.O., Ding S.Y.,
RA   Himmel M.E., Lunin V.V.;
RT   "Structure and function of the Clostridium thermocellum
RT   cellobiohydrolase A X1-module repeat: enhancement through
RT   stabilization of the CbhA complex.";
RL   Acta Crystallogr. D 68:292-299(2012).
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E)
CC       family. {ECO:0000256|RuleBase:RU361166}.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; CP000568; ABN51651.1; -; Genomic_DNA.
DR   RefSeq; WP_003512736.1; NC_009012.1.
DR   PDB; 3PDD; X-ray; 1.72 A; A=823-1003.
DR   PDB; 3PDG; X-ray; 1.78 A; A=823-911.
DR   PDB; 3PE9; X-ray; 1.69 A; A/B/C/D=915-1003.
DR   PDBsum; 3PDD; -.
DR   PDBsum; 3PDG; -.
DR   PDBsum; 3PE9; -.
DR   ProteinModelPortal; A3DCH2; -.
DR   SMR; A3DCH2; -.
DR   STRING; 203119.Cthe_0413; -.
DR   CAZy; CBM3; Carbohydrate-Binding Module Family 3.
DR   CAZy; CBM4; Carbohydrate-Binding Module Family 4.
DR   CAZy; GH9; Glycoside Hydrolase Family 9.
DR   EnsemblBacteria; ABN51651; ABN51651; Cthe_0413.
DR   KEGG; cth:Cthe_0413; -.
DR   eggNOG; ENOG4105E08; Bacteria.
DR   eggNOG; COG2730; LUCA.
DR   eggNOG; COG3979; LUCA.
DR   HOGENOM; HOG000245359; -.
DR   KO; K01179; -.
DR   OMA; YPWHTCE; -.
DR   OrthoDB; POG091H04TS; -.
DR   BioCyc; MetaCyc:MONOMER-16451; -.
DR   Proteomes; UP000002145; Chromosome.
DR   GO; GO:0008810; F:cellulase activity; IEA:InterPro.
DR   GO; GO:0030248; F:cellulose binding; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0000272; P:polysaccharide catabolic process; IEA:InterPro.
DR   CDD; cd02850; E_set_Cellulase_N; 1.
DR   Gene3D; 2.60.120.260; -; 1.
DR   Gene3D; 2.60.40.10; -; 2.
DR   Gene3D; 2.60.40.710; -; 1.
DR   InterPro; IPR008928; 6-hairpin_glycosidase-like.
DR   InterPro; IPR008965; Carb-bd_dom_sf.
DR   InterPro; IPR001956; CBM3.
DR   InterPro; IPR036966; CBM3_sf.
DR   InterPro; IPR004197; Cellulase_Ig-like.
DR   InterPro; IPR003305; CenC_carb-bd.
DR   InterPro; IPR002105; Dockerin_1_rpt.
DR   InterPro; IPR016134; Dockerin_dom.
DR   InterPro; IPR036439; Dockerin_dom_sf.
DR   InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR   InterPro; IPR008979; Galactose-bd-like_sf.
DR   InterPro; IPR001701; Glyco_hydro_9.
DR   InterPro; IPR033126; Glyco_hydro_9_Asp/Glu_AS.
DR   InterPro; IPR018221; Glyco_hydro_9_His_AS.
DR   InterPro; IPR013783; Ig-like_fold.
DR   InterPro; IPR014756; Ig_E-set.
DR   Pfam; PF00942; CBM_3; 1.
DR   Pfam; PF02018; CBM_4_9; 1.
DR   Pfam; PF02927; CelD_N; 1.
DR   Pfam; PF00404; Dockerin_1; 2.
DR   Pfam; PF00759; Glyco_hydro_9; 1.
DR   SMART; SM01067; CBM_3; 1.
DR   SUPFAM; SSF48208; SSF48208; 1.
DR   SUPFAM; SSF49384; SSF49384; 1.
DR   SUPFAM; SSF49785; SSF49785; 1.
DR   SUPFAM; SSF63446; SSF63446; 1.
DR   SUPFAM; SSF81296; SSF81296; 1.
DR   PROSITE; PS51172; CBM3; 1.
DR   PROSITE; PS00448; CLOS_CELLULOSOME_RPT; 1.
DR   PROSITE; PS51766; DOCKERIN; 1.
DR   PROSITE; PS00018; EF_HAND_1; 1.
DR   PROSITE; PS00592; GLYCOSYL_HYDROL_F9_1; 1.
DR   PROSITE; PS00698; GLYCOSYL_HYDROL_F9_2; 1.
PE   1: Evidence at protein level;
KW   3D-structure {ECO:0000213|PDB:3PDD, ECO:0000213|PDB:3PDG,
KW   ECO:0000213|PDB:3PE9}; Calcium {ECO:0000213|PDB:3PDD};
KW   Complete proteome {ECO:0000313|Proteomes:UP000002145};
KW   Glycosidase {ECO:0000256|RuleBase:RU361166};
KW   Hydrolase {ECO:0000256|RuleBase:RU361166,
KW   ECO:0000313|EMBL:ABN51651.1}; Metal-binding {ECO:0000213|PDB:3PDD};
KW   Reference proteome {ECO:0000313|Proteomes:UP000002145};
KW   Signal {ECO:0000256|RuleBase:RU361166}.
FT   SIGNAL        1     27       {ECO:0000256|RuleBase:RU361166}.
FT   CHAIN        28   1224       Glucanase. {ECO:0000256|RuleBase:
FT                                RU361166}.
FT                                /FTId=PRO_5005121352.
FT   DOMAIN     1001   1147       CBM3. {ECO:0000259|PROSITE:PS51172}.
FT   DOMAIN     1157   1223       Dockerin. {ECO:0000259|PROSITE:PS51766}.
FT   METAL       867    867       Calcium 1. {ECO:0000213|PDB:3PDD}.
FT   METAL       869    869       Calcium 1. {ECO:0000213|PDB:3PDD}.
FT   METAL       871    871       Calcium 1; via carbonyl oxygen.
FT                                {ECO:0000213|PDB:3PDD}.
FT   METAL       911    911       Calcium 2; via carbonyl oxygen.
FT                                {ECO:0000213|PDB:3PDD}.
FT   METAL       940    940       Calcium 2. {ECO:0000213|PDB:3PDD}.
FT   METAL       942    942       Calcium 2. {ECO:0000213|PDB:3PDD}.
FT   METAL       960    960       Calcium 3. {ECO:0000213|PDB:3PDD}.
FT   METAL       962    962       Calcium 3; via carbonyl oxygen.
FT                                {ECO:0000213|PDB:3PDD}.
FT   METAL       964    964       Calcium 3; via carbonyl oxygen.
FT                                {ECO:0000213|PDB:3PDD}.
FT   METAL       984    984       Calcium 2. {ECO:0000213|PDB:3PDD}.
SQ   SEQUENCE   1224 AA;  137116 MW;  AAC9B21F76E5A373 CRC64;
     MKFRRSICTA VLLAVLLTLL VPTSVFALED NSSTLPPYKN DLLYERTFDE GLCYPWHTCE
     DSGGKCSFDV VDVPGQPGNK AFAVTVLDKG QNRWSVQMRH RGLTLEQGHT YRVRLKIWAD
     ASCKVYIKIG QMGEPYAEYW NNKWSPYTLT AGKVLEIDET FVMDKPTDDT CEFTFHLGGE
     LAATPPYTVY LDDVSLYDPE YTKPVEYILP QPDVRVNQVG YLPEGKKVAT VVCNSTQPVK
     WQLKNAAGVV VLEGYTEPKG LDKDSQDYVH WLDFSDFATE GIGYYFELPT VNSPTNYSHP
     FDIRKDIYTQ MKYDALAFFY HKRSGIPIEM PYAGGEQWTR PAGHIGIEPN KGDTNVPTWP
     QDDEYAGIPQ KNYTKDVTGG WYDAGDHGKY VVNGGIAVWT LMNMYERAKI RGLDNWGPYR
     DGGMNIPEQN NGYPDILDEA RWEIEFFKKM QVTEKEDPSI AGMVHHKIHD FRWTALGMLP
     HEDPQPRYLR PVSTAATLNF AATLAQSARL WKDYDPTFAA DCLEKAEIAW QAALKHPDIY
     AEYTPGSGGP GGGPYNDDYV GDEFYWAACE LYVTTGKDEY KNYLMNSPHY LEMPAKMGEN
     GGANGEDNGL WGCFTWGTTQ GLGTITLALV ENGLPATDIQ KARNNIAKAA DRWLENIEEQ
     GYRLPIKQAE DERGGYPWGS NSFILNQMIV MGYAYDFTGD SKYLDGMFDG ISYLLGRNAM
     DQSYVTGYGE RPLQNPHDRF WTPQTSKRFP APPPGIISGG PNSRFEDPTI NAAVKKDTPP
     QKCFIDHTDS WSTNEITVNW NAPFAWVTAY LDEQYTDSET DKVTIDSPVA GERFEAGKDI
     NISATVKSKT PVSKVEFYNG DTLISSDTTA PYTAKITGAA VGAYNLKAVA VLSDGRRIES
     PVTPVLVKVI VKPTVKLTAP KSNVVAYGNE FLKITATASD SDGKISRVDF LVDGEVIGSD
     REAPYEYEWK AVEGNHEISV IAYDDDDAAS TPDSVKIFVK QARDVKVQYL CENTQTSTQE
     IKGKFNIVNT GNRDYSLKDI VLRYYFTKEH NSQLQFICYY TPIGSGNLIP SFGGSGDEHY
     LQLEFKDVKL PAGGQTGEIQ FVIRYADNSF HDQSNDYSFD PTIKAFQDYG KVTLYKNGEL
     VWGTPPGGTE PEEPEEPAIV YGDCNDDGKV NSTDVAVMKR YLKKENVNIN LDNADVNADG
     KVNSTDFSIL KRYVMKNIEE LPYR
//
DBGET integrated database retrieval system