ID A3LZT5_PICST Unreviewed; 437 AA.
AC A3LZT5;
DT 03-APR-2007, integrated into UniProtKB/TrEMBL.
DT 03-APR-2007, sequence version 1.
DT 27-MAR-2024, entry version 86.
DE SubName: Full=Putative ribonuclease methylating protein {ECO:0000313|EMBL:ABN68396.1};
GN ORFNames=PICST_50882 {ECO:0000313|EMBL:ABN68396.1};
OS Scheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL
OS Y-11545) (Yeast) (Pichia stipitis).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Debaryomycetaceae; Scheffersomyces.
OX NCBI_TaxID=322104 {ECO:0000313|EMBL:ABN68396.1, ECO:0000313|Proteomes:UP000002258};
RN [1] {ECO:0000313|EMBL:ABN68396.1, ECO:0000313|Proteomes:UP000002258}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545
RC {ECO:0000313|Proteomes:UP000002258};
RX PubMed=17334359; DOI=10.1038/nbt1290;
RA Jeffries T.W., Grigoriev I.V., Grimwood J., Laplaza J.M., Aerts A.,
RA Salamov A., Schmutz J., Lindquist E., Dehal P., Shapiro H., Jin Y.S.,
RA Passoth V., Richardson P.M.;
RT "Genome sequence of the lignocellulose-bioconverting and xylose-fermenting
RT yeast Pichia stipitis.";
RL Nat. Biotechnol. 25:319-326(2007).
CC -!- FUNCTION: Catalytic subunit of an S-adenosyl-L-methionine-dependent
CC tRNA methyltransferase complex that mediates the methylation of the
CC guanosine nucleotide at position 10 (m2G10) in tRNAs.
CC {ECO:0000256|ARBA:ARBA00002425}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CP000502; ABN68396.1; -; Genomic_DNA.
DR RefSeq; XP_001386425.1; XM_001386388.1.
DR AlphaFoldDB; A3LZT5; -.
DR STRING; 322104.A3LZT5; -.
DR GeneID; 4841047; -.
DR KEGG; pic:PICST_50882; -.
DR eggNOG; KOG2671; Eukaryota.
DR HOGENOM; CLU_029646_3_0_1; -.
DR InParanoid; A3LZT5; -.
DR OMA; QAFNKWS; -.
DR OrthoDB; 5488599at2759; -.
DR Proteomes; UP000002258; Chromosome 8.
DR GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR GO; GO:0009451; P:RNA modification; IEA:UniProt.
DR GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR InterPro; IPR000241; RlmKL-like_Mtase.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR016691; tRNA_mtfrase_TRM11.
DR PANTHER; PTHR13370; RNA METHYLASE-RELATED; 1.
DR PANTHER; PTHR13370:SF3; TRNA (GUANINE(10)-N2)-METHYLTRANSFERASE HOMOLOG; 1.
DR Pfam; PF01170; UPF0020; 1.
DR PIRSF; PIRSF017259; tRNA_mtfrase_TRM11; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS00092; N6_MTASE; 1.
DR PROSITE; PS51627; SAM_MT_TRM11; 1.
PE 4: Predicted;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603};
KW Reference proteome {ECO:0000313|Proteomes:UP000002258};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022555};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW tRNA-binding {ECO:0000256|ARBA:ARBA00022555}.
FT DOMAIN 183..298
FT /note="UPF0020"
FT /evidence="ECO:0000259|Pfam:PF01170"
SQ SEQUENCE 437 AA; 50350 MW; D62E52D33EBC3CD8 CRC64;
MKNYLIYFAQ SYPNFRRPEL ESLANLNNFS FDLSHHDESK PFIVVQLEND DQAKKIMDRS
ILSRGIYELW GQGSNYDELH ANIKATSQDK FEVYKKSSFK FDFIGYKGSK SREERVEIIQ
NFKYLAFEGG VRLKNPDEVF TVLEEYSVSG MEKATTPRNI WFGRQVQLSS RTENVLDKYD
LRRRKYIGTT SFDAELSLVT CNIGQVGPGK VCYDPFTGTG SFLVASANFG ALPIGSDIDV
RMLRGKGKNC NIQANFKQYG TSTQFLDVLT MDFTNNAFRK DFVIDSIVCD PPYGVREGLK
VCGAKHPEKA EGRENIVIEG EKAHLRRDFI QPKKPYELSD LLDDLLFFAG ERLPIGGRLA
FWMPTANDDF QVNLIPQHEQ LELLYNLEQE FNKWSRRLLV YVKRDETYKG KTTNGLKSQN
IANFRDRYFN GFNDQSK
//
