ID A3R6J6_9INFA Unreviewed; 480 AA.
AC A3R6J6;
DT 03-APR-2007, integrated into UniProtKB/TrEMBL.
DT 03-APR-2007, sequence version 1.
DT 27-MAR-2024, entry version 56.
DE RecName: Full=RNA-directed RNA polymerase catalytic subunit {ECO:0000256|ARBA:ARBA00020035};
DE EC=2.7.7.48 {ECO:0000256|ARBA:ARBA00012494};
DE Flags: Fragment;
GN Name=PB1 {ECO:0000313|EMBL:ABM46525.1};
OS Influenza A virus (A/quail/Shantou/2816/2000(H9N2)).
OC Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina;
OC Insthoviricetes; Articulavirales; Orthomyxoviridae; Alphainfluenzavirus;
OC Alphainfluenzavirus influenzae; Influenza A virus.
OX NCBI_TaxID=417780 {ECO:0000313|EMBL:ABM46525.1};
RN [1] {ECO:0000313|EMBL:ABM46525.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=A/quail/Shantou/2816/2000 {ECO:0000313|EMBL:ABM46525.1};
RX PubMed=17192315; DOI=10.1128/JVI.02316-06;
RA Xu K.M., Li K.S., Smith G.J., Li J.W., Tai H., Zhang J.X., Webster R.G.,
RA Peiris J.S., Chen H., Guan Y.;
RT "Evolution and molecular epidemiology of H9N2 influenza A viruses from
RT quail in southern China, 2000 to 2005.";
RL J. Virol. 81:2635-2645(2007).
CC -!- FUNCTION: RNA-dependent RNA polymerase which is responsible for
CC replication and transcription of virus RNA segments. The transcription
CC of viral mRNAs occurs by a unique mechanism called cap-snatching. 5'
CC methylated caps of cellular mRNAs are cleaved after 10-13 nucleotides
CC by PA. In turn, these short capped RNAs are used as primers by PB1 for
CC transcription of viral mRNAs. During virus replication, PB1 initiates
CC RNA synthesis and copy vRNA into complementary RNA (cRNA) which in turn
CC serves as a template for the production of more vRNAs.
CC {ECO:0000256|ARBA:ARBA00002148}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}. Host
CC cytoplasm {ECO:0000256|ARBA:ARBA00004192}. Host nucleus
CC {ECO:0000256|ARBA:ARBA00004147}. Nucleus
CC {ECO:0000256|ARBA:ARBA00004123}.
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DR EMBL; EF155351; ABM46525.1; -; Viral_cRNA.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0003968; F:RNA-dependent RNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0039523; P:suppression by virus of host mRNA transcription via inhibition of RNA polymerase II activity; IEA:UniProtKB-KW.
DR GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR GO; GO:0019083; P:viral transcription; IEA:UniProtKB-KW.
DR InterPro; IPR007099; RNA-dir_pol_NSvirus.
DR InterPro; IPR001407; RNA_pol_PB1_influenza.
DR Pfam; PF00602; Flu_PB1; 1.
DR PROSITE; PS50525; RDRP_SSRNA_NEG_SEG; 1.
PE 4: Predicted;
KW Eukaryotic host gene expression shutoff by virus
KW {ECO:0000256|ARBA:ARBA00023247};
KW Eukaryotic host transcription shutoff by virus
KW {ECO:0000256|ARBA:ARBA00022731};
KW Host cytoplasm {ECO:0000256|ARBA:ARBA00023200};
KW Host gene expression shutoff by virus {ECO:0000256|ARBA:ARBA00022995};
KW Host nucleus {ECO:0000256|ARBA:ARBA00022562};
KW Host-virus interaction {ECO:0000256|ARBA:ARBA00022581};
KW Inhibition of host RNA polymerase II by virus
KW {ECO:0000256|ARBA:ARBA00023103}; Membrane {ECO:0000256|SAM:Phobius};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW RNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022484};
KW Transferase {ECO:0000256|ARBA:ARBA00022484};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius};
KW Viral RNA replication {ECO:0000256|ARBA:ARBA00022953};
KW Viral transcription {ECO:0000256|ARBA:ARBA00023314}.
FT TRANSMEM 400..420
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 280..477
FT /note="RdRp catalytic"
FT /evidence="ECO:0000259|PROSITE:PS50525"
FT REGION 47..76
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:ABM46525.1"
FT NON_TER 480
FT /evidence="ECO:0000313|EMBL:ABM46525.1"
SQ SEQUENCE 480 AA; 54552 MW; 639BA12682151C9A CRC64;
LLFLKVPAQN AISTTFPYTG DPPYSHGTGT GYTMDTVNRT HQYSEKGRWT TNTETGAPQL
NPIDGPLPED NEPSGYAQTD CVLEAMAFLE ESHPGLFENS CLETMEVVQQ TRVDKLTQGR
QTYDWTLNRN QPAATALANT IEVFRSNSLT ANESGRLIDF LKDVMESMDK EEMEITTHFQ
RKRRVRDNMT KKMVTQRTIG KKKQKLTKKS YLIRALTLNT MTKDAERGKL KRRAIATPGM
QIRGFVHFVE ALARSICEKL EQSGLPVGGN EKKAKLANVV RKMMTNSQDT ELSFTVTGDN
TKWNENQNPR IFLAMITYIT RNQPEWFRNV LSIAPIMFSN KMARLGKGYM FESKSMKLRT
QIPAEMLANI DLKYFNESTR KKIEKIRPLL IEGTASLSPG MMMGMFNMLS TVLGVSILNL
GQKRYTKTTY WWDGLQSSDD FALIVNAPNH EGIQAGVDRF YRTCKLVGIN MSKKKSYINR
//
