ID A4F953_SACEN Unreviewed; 291 AA.
AC A4F953;
DT 17-APR-2007, integrated into UniProtKB/TrEMBL.
DT 17-APR-2007, sequence version 1.
DT 27-MAR-2024, entry version 84.
DE SubName: Full=4-hydroxy-tetrahydrodipicolinate synthase {ECO:0000313|EMBL:PFG94370.1};
DE SubName: Full=Dihydrodipicolinate synthase {ECO:0000313|EMBL:CAM00578.1};
DE EC=4.2.1.52 {ECO:0000313|EMBL:CAM00578.1};
GN Name=dapA4 {ECO:0000313|EMBL:CAM00578.1};
GN OrderedLocusNames=SACE_1253 {ECO:0000313|EMBL:CAM00578.1};
GN ORFNames=A8924_1645 {ECO:0000313|EMBL:PFG94370.1};
OS Saccharopolyspora erythraea (strain ATCC 11635 / DSM 40517 / JCM 4748 /
OS NBRC 13426 / NCIMB 8594 / NRRL 2338).
OC Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
OC Pseudonocardiaceae; Saccharopolyspora.
OX NCBI_TaxID=405948 {ECO:0000313|EMBL:CAM00578.1, ECO:0000313|Proteomes:UP000006728};
RN [1] {ECO:0000313|EMBL:CAM00578.1, ECO:0000313|Proteomes:UP000006728}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 11635 / DSM 40517 / JCM 4748 / NBRC 13426 / NCIMB 8594 /
RC NRRL 2338 {ECO:0000313|Proteomes:UP000006728}, and NRRL 2338
RC {ECO:0000313|EMBL:CAM00578.1};
RX PubMed=17369815; DOI=10.1038/nbt1297;
RA Oliynyk M., Samborskyy M., Lester J.B., Mironenko T., Scott N., Dickens S.,
RA Haydock S.F., Leadlay P.F.;
RT "Complete genome sequence of the erythromycin-producing bacterium
RT Saccharopolyspora erythraea NRRL23338.";
RL Nat. Biotechnol. 25:447-453(2007).
RN [2] {ECO:0000313|EMBL:PFG94370.1, ECO:0000313|Proteomes:UP000225825}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 40517 {ECO:0000313|EMBL:PFG94370.1,
RC ECO:0000313|Proteomes:UP000225825};
RA Klenk H.-P.;
RT "Sequencing the genomes of 1000 actinobacteria strains.";
RL Submitted (SEP-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the DapA family.
CC {ECO:0000256|PIRNR:PIRNR001365}.
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DR EMBL; AM420293; CAM00578.1; -; Genomic_DNA.
DR EMBL; PDBV01000001; PFG94370.1; -; Genomic_DNA.
DR RefSeq; WP_009945409.1; NZ_PDBV01000001.1.
DR AlphaFoldDB; A4F953; -.
DR STRING; 405948.SACE_1253; -.
DR KEGG; sen:SACE_1253; -.
DR eggNOG; COG0329; Bacteria.
DR HOGENOM; CLU_049343_5_0_11; -.
DR OrthoDB; 9778880at2; -.
DR Proteomes; UP000006728; Chromosome.
DR Proteomes; UP000225825; Unassembled WGS sequence.
DR GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR CDD; cd00408; DHDPS-like; 1.
DR Gene3D; 3.20.20.70; Aldolase class I; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR002220; DapA-like.
DR PANTHER; PTHR12128:SF73; 4-HYDROXY-TETRAHYDRODIPICOLINATE SYNTHASE; 1.
DR PANTHER; PTHR12128; DIHYDRODIPICOLINATE SYNTHASE; 1.
DR Pfam; PF00701; DHDPS; 1.
DR PIRSF; PIRSF001365; DHDPS; 1.
DR PRINTS; PR00146; DHPICSNTHASE.
DR SMART; SM01130; DHDPS; 1.
DR SUPFAM; SSF51569; Aldolase; 1.
PE 3: Inferred from homology;
KW Lyase {ECO:0000256|ARBA:ARBA00023239, ECO:0000256|PIRNR:PIRNR001365};
KW Reference proteome {ECO:0000313|Proteomes:UP000006728}.
FT ACT_SITE 131
FT /note="Proton donor/acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR001365-1"
FT ACT_SITE 159
FT /note="Schiff-base intermediate with substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001365-1"
FT BINDING 44
FT /ligand="pyruvate"
FT /ligand_id="ChEBI:CHEBI:15361"
FT /evidence="ECO:0000256|PIRSR:PIRSR001365-2"
SQ SEQUENCE 291 AA; 30023 MW; 58211FFBE40FA907 CRC64;
MFTGLSAFPL TPTDESGVDE KAFAGLVARL AEAGVDSIGA LGSTGGYAYL TREERARAAR
TAVDAAGGVP VVVGIGALRT SQVRALAEDA QQAGASALLL APVSYQPLTD DEVFGLYEDI
SREVSVPLCV YDNPGTTHFT FSDELHGRIA ALPNIGAVKI PGIPADQAPD RIAALRSTLP
GHAAIGVSGD AFAAGGLSAG ADLWFSVIGG LLPQQCLAIT RAAADGDADR ATALSERMEP
LWALFRRYGS QRVTSAAAEH LGLVSHPNLP KPLRGLDAAA RREVADALEA L
//
