ID A4FP72_SACEN Unreviewed; 873 AA.
AC A4FP72;
DT 17-APR-2007, integrated into UniProtKB/TrEMBL.
DT 17-APR-2007, sequence version 1.
DT 27-MAR-2024, entry version 105.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN Name=uvrD {ECO:0000313|EMBL:CAM05847.1};
GN OrderedLocusNames=SACE_6681 {ECO:0000313|EMBL:CAM05847.1};
GN ORFNames=A8924_7034 {ECO:0000313|EMBL:PFG99489.1};
OS Saccharopolyspora erythraea (strain ATCC 11635 / DSM 40517 / JCM 4748 /
OS NBRC 13426 / NCIMB 8594 / NRRL 2338).
OC Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
OC Pseudonocardiaceae; Saccharopolyspora.
OX NCBI_TaxID=405948 {ECO:0000313|EMBL:CAM05847.1, ECO:0000313|Proteomes:UP000006728};
RN [1] {ECO:0000313|EMBL:CAM05847.1, ECO:0000313|Proteomes:UP000006728}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 11635 / DSM 40517 / JCM 4748 / NBRC 13426 / NCIMB 8594 /
RC NRRL 2338 {ECO:0000313|Proteomes:UP000006728}, and NRRL 2338
RC {ECO:0000313|EMBL:CAM05847.1};
RX PubMed=17369815; DOI=10.1038/nbt1297;
RA Oliynyk M., Samborskyy M., Lester J.B., Mironenko T., Scott N., Dickens S.,
RA Haydock S.F., Leadlay P.F.;
RT "Complete genome sequence of the erythromycin-producing bacterium
RT Saccharopolyspora erythraea NRRL23338.";
RL Nat. Biotechnol. 25:447-453(2007).
RN [2] {ECO:0000313|EMBL:PFG99489.1, ECO:0000313|Proteomes:UP000225825}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 40517 {ECO:0000313|EMBL:PFG99489.1,
RC ECO:0000313|Proteomes:UP000225825};
RA Klenk H.-P.;
RT "Sequencing the genomes of 1000 actinobacteria strains.";
RL Submitted (SEP-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by
CC translocating in the 3'-5' direction.; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034617};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922}.
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DR EMBL; AM420293; CAM05847.1; -; Genomic_DNA.
DR EMBL; PDBV01000001; PFG99489.1; -; Genomic_DNA.
DR RefSeq; WP_009943395.1; NZ_PDBV01000001.1.
DR AlphaFoldDB; A4FP72; -.
DR STRING; 405948.SACE_6681; -.
DR KEGG; sen:SACE_6681; -.
DR eggNOG; COG0210; Bacteria.
DR HOGENOM; CLU_004585_5_2_11; -.
DR OrthoDB; 9806690at2; -.
DR Proteomes; UP000006728; Chromosome.
DR Proteomes; UP000225825; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-UniRule.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR CDD; cd18807; SF1_C_UvrD; 2.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 3.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF21196; PcrA_UvrD_tudor; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Reference proteome {ECO:0000313|Proteomes:UP000006728}.
FT DOMAIN 32..333
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 334..687
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT REGION 1..33
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 263..288
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 570..642
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 777..808
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..19
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 53..60
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 873 AA; 95429 MW; 282808E08B600857 CRC64;
MSTLFDLPQD GSSGPAETPS QRRGPDPQDL LAGLNPQQRD AVEHSGSPLL VVAGAGSGKT
RVLTNRIAYL LARGVHPGQI MAITFTNKAA AEMKERVADL VGRRANAMWV STFHSMCVRV
LRREAKTLGM SSNFSIYDAD DSRRLITIIA RELELDSKRY PARTLAVHIS NLKNELLTPE
DAAERAGNDV ERKVAQVYTA YQRRLAESNA MDFDDLIMRT VELLQGYPAV AEHYRRRFRH
VLVDEYQDTN HAQYVLVREL VGTNRPGEPD ADSEADADES GEETVEPGEL CVVGDADQSI
YAFRGATIRN IEEFERDYPE ARAILLEQNY RSTQTILSAA NAVIRRNPNR RDKRLWSDAG
DGEQIVGYVG DNEHDEAAFV AGEIDRLVDE GEATFNDIAV FYRTNNQSRV FEEVFIRLGL
PYRVVGGVRF YERREVRDAL AYLRVLDNPE DTVSLRRILN VPKRGIGDRA EAVVAAHAEQ
ERISFAAALR KAAAGEVPLL NTRSQRAIAG FVEMLDELHG IAAESDVADV LEAVLDRTGY
RAELEDSDDP QDATRVENLT ELVTVAREFI EARSPEPAAA PGDNAEDLDD AAGDLGENTS
DLGNSAEDAA APGDGATGDE DAHASAEAVV APAEPAAEDD DEAALGLPAD DSLSAFLERV
SLVADADSLP DSGDGVVTLM TLHTAKGLEF PVVFCTGWED GIFPHMRALG EPAELAEERR
LAYVGITRAR QRLYLSRALM RSAWGQPMTN PASRFLTEIP DELVHWRRIE PERAAPQVRS
TWGRRNSGFD RGGSDSAQAG LAERGMRSTP AKGWKDAVAI KLDAGDRVTH DKYGLGTVIA
TEGEGPRATA TIDFGSSGTV RLMLIGSVPL TKL
//
