ID A4HYL7_LEIIN Unreviewed; 4825 AA.
AC A4HYL7; A0A2K4YSQ0; A0A381MG26;
DT 01-MAY-2007, integrated into UniProtKB/TrEMBL.
DT 05-APR-2011, sequence version 2.
DT 27-MAR-2024, entry version 103.
DE RecName: Full=Midasin {ECO:0000256|PIRNR:PIRNR010340};
GN ORFNames=LINJ_20_0280 {ECO:0000313|EMBL:CAM67405.2};
OS Leishmania infantum.
OC Eukaryota; Discoba; Euglenozoa; Kinetoplastea; Metakinetoplastina;
OC Trypanosomatida; Trypanosomatidae; Leishmaniinae; Leishmania.
OX NCBI_TaxID=5671 {ECO:0000313|EMBL:CAM67405.2, ECO:0000313|Proteomes:UP000008153};
RN [1] {ECO:0000313|EMBL:CAM67405.2, ECO:0000313|Proteomes:UP000008153}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JPCM5 {ECO:0000313|EMBL:CAM67405.2,
RC ECO:0000313|Proteomes:UP000008153};
RX PubMed=17572675; DOI=10.1038/ng2053;
RA Peacock C.S., Seeger K., Harris D., Murphy L., Ruiz J.C., Quail M.A.,
RA Peters N., Adlem E., Tivey A., Aslett M., Kerhornou A., Ivens A.,
RA Fraser A., Rajandream M.A., Carver T., Norbertczak H., Chillingworth T.,
RA Hance Z., Jagels K., Moule S., Ormond D., Rutter S., Squares R.,
RA Whitehead S., Rabbinowitsch E., Arrowsmith C., White B., Thurston S.,
RA Bringaud F., Baldauf S.L., Faulconbridge A., Jeffares D., Depledge D.P.,
RA Oyola S.O., Hilley J.D., Brito L.O., Tosi L.R., Barrell B., Cruz A.K.,
RA Mottram J.C., Smith D.F., Berriman M.;
RT "Comparative genomic analysis of three Leishmania species that cause
RT diverse human disease.";
RL Nat. Genet. 39:839-847(2007).
RN [2] {ECO:0000313|EMBL:CAM67405.2, ECO:0000313|Proteomes:UP000008153}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JPCM5 {ECO:0000313|EMBL:CAM67405.2,
RC ECO:0000313|Proteomes:UP000008153};
RX PubMed=22038252; DOI=10.1101/gr.122945.111;
RA Rogers M.B., Hilley J.D., Dickens N.J., Wilkes J., Bates P.A.,
RA Depledge D.P., Harris D., Her Y., Herzyk P., Imamura H., Otto T.D.,
RA Sanders M., Seeger K., Dujardin J.C., Berriman M., Smith D.F.,
RA Hertz-Fowler C., Mottram J.C.;
RT "Chromosome and gene copy number variation allow major structural change
RT between species and strains of Leishmania.";
RL Genome Res. 21:2129-2142(2011).
CC -!- FUNCTION: Nuclear chaperone required for maturation and nuclear export
CC of pre-60S ribosome subunits. {ECO:0000256|PIRNR:PIRNR010340}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|PIRNR:PIRNR010340}.
CC -!- SIMILARITY: Belongs to the midasin family.
CC {ECO:0000256|PIRNR:PIRNR010340}.
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DR EMBL; FR796452; CAM67405.2; -; Genomic_DNA.
DR RefSeq; XP_001465158.2; XM_001465121.2.
DR STRING; 5671.A4HYL7; -.
DR GeneID; 5068573; -.
DR KEGG; lif:LINJ_20_0280; -.
DR VEuPathDB; TriTrypDB:LINF_200007600; -.
DR eggNOG; KOG1808; Eukaryota.
DR InParanoid; A4HYL7; -.
DR OrthoDB; 1221059at2759; -.
DR Proteomes; UP000008153; Chromosome 20.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR CDD; cd00009; AAA; 2.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 6.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR040848; AAA_lid_7.
DR InterPro; IPR011704; ATPase_dyneun-rel_AAA.
DR InterPro; IPR012099; Midasin.
DR InterPro; IPR041190; Midasin_AAA_lid_5.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR025662; Sigma_54_int_dom_ATP-bd_1.
DR InterPro; IPR036465; vWFA_dom_sf.
DR PANTHER; PTHR48103:SF2; MIDASIN; 1.
DR PANTHER; PTHR48103; MIDASIN-RELATED; 1.
DR Pfam; PF07728; AAA_5; 8.
DR Pfam; PF17865; AAA_lid_5; 1.
DR Pfam; PF17867; AAA_lid_7; 3.
DR PIRSF; PIRSF010340; Midasin; 3.
DR SMART; SM00382; AAA; 6.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 6.
DR SUPFAM; SSF53300; vWA-like; 1.
DR PROSITE; PS00675; SIGMA54_INTERACT_1; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PIRNR:PIRNR010340};
KW Chaperone {ECO:0000256|ARBA:ARBA00023186, ECO:0000256|PIRNR:PIRNR010340};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|PIRNR:PIRNR010340};
KW Nucleus {ECO:0000256|ARBA:ARBA00023242, ECO:0000256|PIRNR:PIRNR010340};
KW Reference proteome {ECO:0000313|Proteomes:UP000008153}.
FT DOMAIN 22..164
FT /note="AAA+ ATPase"
FT /evidence="ECO:0000259|SMART:SM00382"
FT DOMAIN 350..589
FT /note="AAA+ ATPase"
FT /evidence="ECO:0000259|SMART:SM00382"
FT DOMAIN 755..900
FT /note="AAA+ ATPase"
FT /evidence="ECO:0000259|SMART:SM00382"
FT DOMAIN 1051..1206
FT /note="AAA+ ATPase"
FT /evidence="ECO:0000259|SMART:SM00382"
FT DOMAIN 1370..1584
FT /note="AAA+ ATPase"
FT /evidence="ECO:0000259|SMART:SM00382"
FT DOMAIN 1722..1871
FT /note="AAA+ ATPase"
FT /evidence="ECO:0000259|SMART:SM00382"
FT REGION 1422..1458
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3399..3432
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3814..3836
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3952..3983
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3998..4479
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 3998..4015
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4024..4038
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4039..4055
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4056..4074
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4075..4098
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4099..4131
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4144..4169
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4184..4214
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4232..4268
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4276..4290
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4333..4355
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4369..4401
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4414..4459
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 4825 AA; 534934 MW; 48DAEA1075CCA7ED CRC64;
MVMILTPTVT RNLESIQLAL ETSNAILIQG EIGCGKSASV LHLAEKYAAK ETMIQVNVDD
TFDSKDLLGK FSATQTPGTF QWVPGPLTVA VQKGYWVLME DIDLASFDVF AVLLSLLESS
TLFIPDKSKF LRAHSGFRLI ATQQLYSSGA AVLTRKSNSV PYAELWGTVI MENLPAEEMC
AIVNHTYSNV PLSIIQSLAA LASPTSGTRL VGLQNLLRWS KRVSKRLPST LCIDNANSKN
TLAFISSRTR ETMVKEAFDC LVAKYPPGNE RAGVLKLISE ACGVPINVAE PLVLECRPQE
SLTTDMYSVG RVCIPLISTV SGLLRDSRIA FAPTTHAMSL LERLAAAVES HEHVLLTGET
GVGKTFIVQY LADQLGQSLI VHNLNQQTDS SDFMGGWKPL DVGVTVRDLY NGFTELFAAC
FNVEKNAQFL NILQQSVNGR KWGRALSQII KGCGSFRAKS AKVKYDAAMQ QQWEDLEQKA
NELLNTVENT RTNFAFCFEE GSLVKAWREG HWILLDELNL ASTEVLERVS SVLGDVDRLF
LTDKDSSEPI MRHKNFHVFA NMNPPTDVGK KDLPPSLRSR FVEYYVSEPF NRNDISIVVR
AYIGFLTADA KVDEITDFFL DCVNNAKTKL CALDGESKAP SFSLRTLTRA LSYVRRATPL
YGFAIALFDG LLLGFATPLQ RQFHVVVEQL IVKNIFRGKR PNSPTLPAAP KNGSFIAYEH
MWIPAGTEVP YCDEAFILTN SVKGHLTNLS RAVFANRPVL LEGPTSSGKS SMVKYLAEIT
GNKFVRINNH DSTEVQEYLG HYVTDELGKL RYVDGILVKA VRHGYWVVLD ELNLAPTDVL
EALNRLLDDN CELFVADTQE TIKPHPQLRI FATQNPAGIY SGRKMLSRAF RNRFLEITVD
EIPTTEISTI LCRRYSLPQS FADKMVEVMT ALQIHRQSSQ IFAGRHGFIT PRDLFRWAER
RPETYQQLAE HGFLLLGERC RKPSERQVVR DVIESVTKTE LDDKTIYHPS HWLFVQRYFS
QVESGVIDEF KIVWTESMQR LFTIVGICLE HKEPVLLVGE TGSSKTTVCQ LWAALLGIPL
HILNCHQHTE ASDFLGSLRP SPPDRRDQAL FEWRNGPLVQ SMLNGDMFVL DEISLTEDSV
LERLNSVLEP SRSVTLAEKS SADCIVAHER FLVMATMNPG GDFGKKELSP ALRNRFTEIY
VRPTTDVTEV SMILSNRLEA ELAVWAPHMA AVLYHVSQST TTSSSPQHIS IRDIISWVSF
MNAAHGRCPP EVAFLQGLDA VILDGIGVGT GQSEASCAVL KSSCTSQAVQ EVMQSGHAPI
DHNALLAKPF WEVCEGMAEP QLPPSMQGRF FFGAPATRRN LSKLMRASLV KKAVLLEGSP
GVGKTSIVEA LGCALDRSVV RINLSEQTDI MDLFGTYLPS PSDATEQEAA AEGEESAATV
GDELERQQPR GGRGPVKSPQ FAWSDGVLLR ALKQGSWVIL DELNLASQSV LEGLNALLDH
RSSVFIPELN EEFHAHADFR VFACQNPLLE GGGRKGLPCS FINRFIKVHV EAFEVSDLIV
IAQAVCPDIE EATLRRMVNF VDRLQYDIMV RRAYGLRGSP WEFNLRDVLR WAKLLQSYEA
RERPTSFADM LFLQRMRTDE DAARCRLALE SCFSKEEVER DVEHGCDSDF LIHNNTVYFA
KQRLMQRETA TISASEAQQL SSLLLLPSQA HLVKSLLVCV EQNQFALLVG PSGAGKTFAL
RTAAALAGAR LVTFSMTASC DTVDLLGGFD QVEGKQGEFE WRDSLVLEAM LQGHWLVLDN
VNYCNASVLD RLNPLVEPNG VLFINEQGVV NDEVRMIRPH PRFRLFATLN PRYGEISRAM
RNRAVELYVK PVAIPSAESF ALGAHASVVA QERHQSSQDA KSADEAANSV ASLSAPPVRL
LSRLLRYHQT FVSVGFHADS QHPGTAQLSD ALSAGAPSVF TLLRTSCLLR PSSLVGDLER
LILHRYIHNR SGHWASFKSE LLELLRCIMD GDTEENDTAA FADEAMEVLR ALRLGGRGDE
MLDRAAAAED EEAATASDPV ARALVCCRVG RAALALQRLL SESASATEHD MKDAAEAAKQ
KEKRTLTNRH RLEDLVLCFV LEQCSDSVPL DAVTETRRLW ISHRLPAELA QRALSLVRTS
FCSSEMLARL YTRAVLLLGP HADALTALWG SQMQVVSTTL VNNALVHLCG VLQAAFASHN
ATPVPPATFQ QLLRLAAPFA RLVVQLANEE YGVHTDNYIP LVTYTALMCK AASKMKPPIP
HAMALVHAAT SFLDSLQQHL GLPFPLLGGK APLHRYVPTP HITTAEERRL LQAWNTTANE
ASAARPTAQS NSAVGPQALL HRLWESAAFR EGFACLQQLL YIRATAATYS SVTSADPAHT
VHPLLPALQA RMTAFVDTYT ARLPLFTELE LALWRSVHQL SLDKPADTTE ALAQLAPLLL
SRFASRHGVA GTRHLFGGAW AVAAADCMRY GAQVRVMDIG LADNPRLQAL AGLSTFLQTC
PLAAASPLEE WATNSQRELV GVLCTCFPDL ASCVPTPLPP SRTSATALTR LSVFGKVETA
VASAAPMPSL VLERQRVLMA ALLRRLRSNG CDSADTLSAV TLLWVFKCRL LLPQSPVDPM
YRRECKRVVA TEELELLGRL DGAFAWAEQL AIRHRSPQRE ILLTLRVQEK ELLRQAQRKY
VTRPDATGMK YATLIRQLYQ VCTTLLSDAR VLGLFEMPPA SAGGLNAAAA AEIIASREAW
SDLLYEQVMQ LLADYGGYED LTVNFAEAAL LASMAARASC TTDVRLLAAA ASADSSALSR
TLFSRAETLR LLQFPSPLVH VLPMPPLQHT ASLLRHRVVY LDSCLSLLSG TAPQGTSRDF
VEDVFAQYRS LFRQIEEWEV QERQADQLAV MYKEKATVIE GDDEKLIRKL KELYPSYEAE
FSIDALEDDA AESSDRKKTE EQLEGRRAKF ARILFKERDG FYLRHLVEVH RTFYWRLISC
RMKEAATAPS ACSAKSPQST ALLAAFQNRF DALAAELHTF PASSGSTSGV AGMEEQLLLN
GFAARVSLLR EEMRGTAVSL EERKEAAFNI FLDTDVYELS RFSAPLQTLL DAVQRLAEQY
PDTPSLQRCL RIGHKIATLP AMSTPLMKVM AGCEVLLREC YEWERNASHE VSLMRHMTAL
SSFVLRWRRL ELHCWAHVFQ AKRAELELQA AQQWFTLYDL LLGEAVPCEP DGEGHHTVAV
WDAETPLLRH CRTAFQRASK FMWDAIFGDF SVRLRILQGF GFQLLAAESA QAPLANTVLH
VCDFFAQFEP LVQQQRDKAV QPIEEDIAEF AKIMRWEDAN YYAVRATAEK SHMKMARVLG
TLEEVLRTPV LAVITAEEQR CEDDASLGFA LPVKVDSKSK KARQARKPAE AAGKGAKQGI
KRQRNGKAVS AAADNPSLEA VATAEDPALQ LLSRNVYLDQ VHTFLRDAAH EVIENVTALQ
KPKTPNQMKA RALKTLFGRL SEAGVPHTHE NQVGDWGVLF FTTEALLGCC ETHRDQTAAV
ARAGAAAAEQ YRFARWLQRL REVERKPHHD LSAAQAKRGT GIAESLFSNA VHVATVLCEL
FHLDEQLRVL EVTVQHSVLA SDGDTDGGVV SHAVPRVFLD DAVEVACLVQ SVCSGLGWMT
QQPHMVAKTT AEAHGLVHEL AGVSNELWSL CTSYEPLRLS AASTVPPGIS KYYFDALHRV
LGVVCRIQAC DVANIAAAAK PLVDAVTDML ARHEQPSAQM RERLATEAKR RRTEQLPKPW
AQRLRHAVEE VSDALVPCGE GASVVLEDDV RMPSTTATTD AEAKVSGHCD SESDTDAPQL
ESAVYARYTM HGAAVHEALQ RLVPILEDLL ASPVSSEGAT AATVVTSSAL PSVCTLAARE
REELLPRLSV CRREVQRVRD AWCTVLEAQC FFGVVVARLF TVLLKKGFCK TEDEEPDDGE
GDGEGGQQQN GTGMDDGQGE KDVTDQIDNE DQLMNMKDKE EQQADEQRQG EKDEGEEDTA
ADVETDFNGQ KEHRSESDGE GDEEGEESDK EMGDVDDGTE QERKKAKKDA GDADDMNEDA
GDAAEEVPED DLAEGDEHRE DNEETGSFED KADEIRDADE DRSCEHDVVG DDDKSEMDED
GSSDGDSVEH SDTDGEERDE SARDDKLPSD ADEEPEESDV ASEERVDSDA ATDAERSELG
EEEVEQRDNS ENAGDVSDIE DASDGPDENV YEGGQQDDRE GDEATENQDK DKKDTSAKVR
KEQAANEEEQ PGTEQEQDDA GQNWKQQQEQ NEQAQRRDNA EQTRSQHNPY RAVKEALQRH
QRQMQQLNLN QHVKVTDEKD EPKAEKPPKP DQAGEQEDVE EFDFEEDGDR EGLAAAEHTE
AKPTETDAYE GEESHNDASG DEDGIGEDED DSADAPQETR RKCRQNRDEL TELEEKEAAA
TSQKMKSKKK VRTTADDEDA SASEDQEDDD TTKKDDQDAA AEANVKLERG RQLWLEQETA
VHGLSQQLCE QLRLILAPTL ADKLQGDYKT GKRLNMKRII PYIASQFKKD RIWLRRTKPS
KRTYQILVAL DDSLSMQCNN AGVLSCRAVA LIAKALQQLE VGEFGVACFG KETHVVHPLE
EPFLADSGPR LFSEITFEQK STNMKLLLET TLGYLDDARD RMRGQTRSTT QQLQQIMFII
GDGQITENRD DLRQLMVRAE ENHQMVVFVL LDIRAAEGDT ATPSAGPSAA PAAAAPLRAK
TLQGLSTAER LRRLKADREA RLLRVQSNSI LDMQVVEFQA GRVVRRPYME DFPFPYYLII
RDIQNLPEII ADAMRQWFEL LNAGA
//
