ID URE1_BURVG Reviewed; 568 AA.
AC A4JC42;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2007, sequence version 1.
DT 01-MAY-2013, entry version 46.
DE RecName: Full=Urease subunit alpha;
DE EC=3.5.1.5;
DE AltName: Full=Urea amidohydrolase subunit alpha;
GN Name=ureC; OrderedLocusNames=Bcep1808_0833;
OS Burkholderia vietnamiensis (strain G4 / LMG 22486) (Burkholderia
OS cepacia (strain R1808)).
OC Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC Burkholderiaceae; Burkholderia; Burkholderia cepacia complex.
OX NCBI_TaxID=269482;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=G4 / LMG 22486;
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H.,
RA Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J.,
RA Larimer F., Land M., Hauser L., Kyrpides N., Tiedje J., Richardson P.;
RT "Complete sequence of chromosome 1 of Burkholderia vietnamiensis G4.";
RL Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY: Urea + H(2)O = CO(2) + 2 NH(3).
CC -!- COFACTOR: Binds 2 nickel ions per subunit (By similarity).
CC -!- PATHWAY: Nitrogen metabolism; urea degradation; CO(2) and NH(3)
CC from urea (urease route): step 1/1.
CC -!- SUBUNIT: Heterotrimer of UreA (gamma), UreB (beta) and UreC
CC (alpha) subunits. Three heterotrimers associate to form the active
CC enzyme (By similarity).
CC -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC -!- PTM: Carbamylation allows a single lysine to coordinate two nickel
CC ions (By similarity).
CC -!- SIMILARITY: Belongs to the urease family.
CC -!- SIMILARITY: Contains 1 urease domain.
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DR EMBL; CP000614; ABO53845.1; -; Genomic_DNA.
DR RefSeq; YP_001118680.1; NC_009256.1.
DR ProteinModelPortal; A4JC42; -.
DR SMR; A4JC42; 5-568.
DR STRING; 269482.Bcep1808_0833; -.
DR MEROPS; M38.982; -.
DR EnsemblBacteria; ABO53845; ABO53845; Bcep1808_0833.
DR GeneID; 4952529; -.
DR KEGG; bvi:Bcep1808_0833; -.
DR PATRIC; 19320128; VBIBurVie89221_5053.
DR eggNOG; COG0804; -.
DR HOGENOM; HOG000075064; -.
DR KO; K01428; -.
DR OMA; TIHAFHT; -.
DR ProtClustDB; PRK13207; -.
DR BioCyc; BVIE269482:GJNA-856-MONOMER; -.
DR UniPathway; UPA00258; UER00370.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016151; F:nickel cation binding; IEA:HAMAP.
DR GO; GO:0009039; F:urease activity; IEA:HAMAP.
DR GO; GO:0043419; P:urea catabolic process; IEA:UniProtKB-UniPathway.
DR HAMAP; MF_01953; Urease_alpha; 1; -.
DR InterPro; IPR006680; Amidohydro_1.
DR InterPro; IPR011059; Metal-dep_hydrolase_composite.
DR InterPro; IPR011612; Urease_alpha_N.
DR InterPro; IPR005848; Urease_asu.
DR InterPro; IPR017951; Urease_asu_c.
DR InterPro; IPR017950; Urease_asu_CS.
DR Pfam; PF01979; Amidohydro_1; 1.
DR Pfam; PF00449; Urease_alpha; 1.
DR PRINTS; PR01752; UREASE.
DR SUPFAM; SSF51338; Metalo_hydrolase; 2.
DR TIGRFAMs; TIGR01792; urease_alph; 1.
DR PROSITE; PS01120; UREASE_1; 1.
DR PROSITE; PS00145; UREASE_2; 1.
DR PROSITE; PS51368; UREASE_3; 1.
PE 3: Inferred from homology;
KW Complete proteome; Cytoplasm; Hydrolase; Metal-binding; Nickel.
FT CHAIN 1 568 Urease subunit alpha.
FT /FTId=PRO_1000073701.
FT DOMAIN 130 568 Urease.
FT ACT_SITE 321 321 Proton donor (By similarity).
FT METAL 135 135 Nickel 2 (By similarity).
FT METAL 137 137 Nickel 2 (By similarity).
FT METAL 218 218 Nickel 1; via carbamate group (By
FT similarity).
FT METAL 218 218 Nickel 2; via carbamate group (By
FT similarity).
FT METAL 247 247 Nickel 1 (By similarity).
FT METAL 273 273 Nickel 1 (By similarity).
FT METAL 361 361 Nickel 2 (By similarity).
FT BINDING 220 220 Substrate (By similarity).
FT MOD_RES 218 218 N6-carboxylysine (By similarity).
SQ SEQUENCE 568 AA; 61031 MW; 73EF5C10FCF33794 CRC64;
MTLRLSRRAY AEMFGPTTGD RVRLADTELL IEIERDYTIY GEEVKFGGGK VIRDGMGQSQ
RVAADVPDTI ITNAVILDHW GIVKADIAIK HGRIAAIGKA GNPDIQPGVT IAIGAATEVI
AGEGLIVTAG GIDTHIHFIS PQQIDEALAS GVTTMLGGGT GPATGTNATT CTPGPWHMER
MLQAADGWPI NLGFLGKGNV SVPQPLVEQI AAGAIGLKLH EDWGTTPAAI DNCLSVADDT
DTQVAIHTDT LNEGGFVEST VAAFKGRTIH TYHTEGAGGG HAPDILKVCG ESNVLPSSTN
PTRPYTINTL DEHLDMLMVC HHLDPSIAED LAFAESRIRR ETIAAEDILH DLGALSMLSS
DSQAMGRVGE VIIRTWQTAH KMKVQRGALP EDNARNDNFR AKRYVAKYTI NPAITHGIAH
EVGSIEPGKW ADLVLWEPAF FGIKPSMILK GGMIAMAQMG DPNASIPTPQ PVHYREMFAT
RGGALARTSL TFVSQMAADA GIAERYGLAK WIVPVRNCRN VTKADMIHNA WRPSISVDPE
TYDVIADGQL LTCEPATVLP MAQRYFLF
//
