ID MAO1_YERPP Reviewed; 565 AA.
AC A4TKN8;
DT 05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 15-MAY-2007, sequence version 1.
DT 01-MAY-2013, entry version 48.
DE RecName: Full=NAD-dependent malic enzyme;
DE Short=NAD-ME;
DE EC=1.1.1.38;
GN Name=maeA; OrderedLocusNames=YPDSF_1463;
OS Yersinia pestis (strain Pestoides F).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC Enterobacteriaceae; Yersinia.
OX NCBI_TaxID=386656;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Pestoides F;
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H.,
RA Pitluck S., Di Bartolo G., Chain P., Malfatti S., Shin M., Vergez L.,
RA Schmutz J., Larimer F., Land M., Hauser L., Worsham P., Chu M.,
RA Bearden S., Garcia E., Richardson P.;
RT "Complete sequence of chromosome of Yersinia pestis Pestoides F.";
RL Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY: (S)-malate + NAD(+) = pyruvate + CO(2) + NADH.
CC -!- CATALYTIC ACTIVITY: Oxaloacetate = pyruvate + CO(2).
CC -!- COFACTOR: Divalent metal cations. Prefers magnesium or manganese
CC (By similarity).
CC -!- SUBUNIT: Homotetramer (By similarity).
CC -!- SIMILARITY: Belongs to the malic enzymes family.
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DR EMBL; CP000668; ABP39850.1; -; Genomic_DNA.
DR RefSeq; YP_001162823.1; NC_009381.1.
DR ProteinModelPortal; A4TKN8; -.
DR STRING; 386656.YPDSF_1463; -.
DR EnsemblBacteria; ABP39850; ABP39850; YPDSF_1463.
DR GeneID; 5064121; -.
DR KEGG; ypp:YPDSF_1463; -.
DR PATRIC; 18612147; VBIYerPes122972_1985.
DR eggNOG; COG0281; -.
DR HOGENOM; HOG000042487; -.
DR KO; K00027; -.
DR OMA; HCERPIV; -.
DR ProtClustDB; PRK13529; -.
DR GO; GO:0016619; F:malate dehydrogenase (oxaloacetate-decarboxylating) activity; IEA:HAMAP.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR GO; GO:0006108; P:malate metabolic process; IEA:InterPro.
DR Gene3D; 3.40.50.10380; -; 1.
DR Gene3D; 3.40.50.720; -; 1.
DR HAMAP; MF_01619; NAD_malic_enz; 1; -.
DR InterPro; IPR015884; Malic_enzyme_CS.
DR InterPro; IPR012301; Malic_N.
DR InterPro; IPR012302; Malic_NAD-bd.
DR InterPro; IPR001891; Malic_OxRdtase.
DR InterPro; IPR016040; NAD(P)-bd_dom.
DR InterPro; IPR023667; NAD_malic_enz_proteobac.
DR Pfam; PF00390; malic; 1.
DR Pfam; PF03949; Malic_M; 1.
DR PIRSF; PIRSF000106; ME; 1.
DR PRINTS; PR00072; MALOXRDTASE.
DR SMART; SM00919; Malic_M; 1.
DR PROSITE; PS00331; MALIC_ENZYMES; 1.
PE 3: Inferred from homology;
KW Complete proteome; Metal-binding; NAD; Oxidoreductase.
FT CHAIN 1 565 NAD-dependent malic enzyme.
FT /FTId=PRO_1000069556.
FT ACT_SITE 104 104 Proton donor (By similarity).
FT ACT_SITE 175 175 Proton acceptor (By similarity).
FT METAL 246 246 Divalent metal cation (By similarity).
FT METAL 247 247 Divalent metal cation (By similarity).
FT METAL 270 270 Divalent metal cation (By similarity).
FT BINDING 157 157 NAD (By similarity).
FT BINDING 270 270 NAD (By similarity).
FT BINDING 418 418 NAD (By similarity).
FT SITE 270 270 Important for activity (By similarity).
SQ SEQUENCE 565 AA; 62828 MW; F57C1183EEC4AA3D CRC64;
MELEYESKRP LYIPYAGPIL LEFPLLNKGS AFTNDERNHF NLHGLLPEAV ETIEEQAERA
YRQYQDFKND DDKHIYLRNI QDTNETLFYR LLEAHLSEMM PIIYTPTVGE ACEHFSDIYR
RARGLFISYP NREHIDDMLQ NATKQNVKVI VVTDGERILG LGDQGIGGMG IPIGKLSLYT
ACGGISPAYT LPVVLDVGTN NPQRLNDPLY MGWRHPRISG DEYYAFVDEF IQAVKRRWPN
VLLQFEDFAQ KNATPLLNRY RDELCCFNDD IQGTAAVTLG SLIAASHAAG SQLRDQTVTF
LGAGSAGCGI AEQIIAQMMS EGLSEIQARA RIFMVDRFGL LTDKLPNLLD FQSKLVQKSD
DLHHWNLHND AISLLDVVRN AKPTVLIGVS GQPGLFTEEL IREMHSHCAR PIVMPLSNPT
SRVEARPEDI INWTDGAALV ATGSPFPPVS YKEKLYPIAQ CNNSYIFPGI GLGVLASGAS
RVTDGMLMAA SRALAESSPL ARHGEGALLP NIDDIQAVSK AIAMRVGQAA QLQGVAIVTS
EEALSKAIEH NYWQPQYRSY KRTSF
//
