ID A4V820_PSEFS Unreviewed; 1121 AA.
AC A4V820;
DT 29-MAY-2007, integrated into UniProtKB/TrEMBL.
DT 29-MAY-2007, sequence version 1.
DT 27-MAR-2024, entry version 118.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
DE AltName: Full=DNA 3'-5' helicase II {ECO:0000256|ARBA:ARBA00034923};
GN Name=helA {ECO:0000313|EMBL:CAM96087.1};
GN OrderedLocusNames=pQBR0055 {ECO:0000313|EMBL:CAM96087.1};
OS Pseudomonas fluorescens (strain SBW25).
OG Plasmid pQBR103 {ECO:0000313|EMBL:CAM96087.1,
OG ECO:0000313|Proteomes:UP000002332}.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=216595 {ECO:0000313|EMBL:CAM96087.1, ECO:0000313|Proteomes:UP000002332};
RN [1] {ECO:0000313|EMBL:CAM96087.1, ECO:0000313|Proteomes:UP000002332}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SBW25 {ECO:0000313|EMBL:CAM96087.1,
RC ECO:0000313|Proteomes:UP000002332};
RX PubMed=18043644; DOI=10.1038/ismej.2007.47;
RA Tett A., Spiers A.J., Crossman L.C., Ager D., Ciric L., Dow J.M., Fry J.C.,
RA Harris D., Lilley A., Oliver A., Parkhill J., Quail M.A., Rainey P.B.,
RA Saunders N.J., Seeger K., Snyder L.A.S., Squares R., Thomas C.M.,
RA Turner S.L., Zhang X.-X., Field D., Bailey M.J.;
RT "Sequence-based analysis of pQBR103; a representative of a unique,
RT transfer-proficient mega plasmid resident in the microbial community of
RT sugar beet.";
RL ISME J. 1:331-340(2007).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
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DR EMBL; AM235768; CAM96087.1; -; Genomic_DNA.
DR RefSeq; WP_011922864.1; NC_009444.1.
DR RefSeq; YP_001201803.1; NC_009444.1.
DR AlphaFoldDB; A4V820; -.
DR PATRIC; fig|216595.4.peg.32; -.
DR Proteomes; UP000002332; Plasmid pQBR103.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-UniRule.
DR Gene3D; 3.90.320.10; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 3.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR011604; PDDEXK-like_dom_sf.
DR InterPro; IPR011335; Restrct_endonuc-II-like.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF52980; Restriction endonuclease-like; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 4: Predicted;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; DNA damage {ECO:0000256|ARBA:ARBA00022763};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Exonuclease {ECO:0000256|ARBA:ARBA00022839};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Plasmid {ECO:0000313|EMBL:CAM96087.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000002332}.
FT DOMAIN 24..501
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 505..764
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT BINDING 45..52
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 1121 AA; 122993 MW; 76D184C40153D7FB CRC64;
MTEFVPFAAL PHLDPIFAGI RGLSIADAEA RALAIDPRRS LLLLAPAGSG KTSTLQMRLL
SCLTVVERPE EVLAITFTNM AAAEIVERVI GALQQAATGI EPELVHELPQ YRLARLVLDR
DKEMGWNLLL NPSRLRIMTF DSFCASLASK TPIMSGLGGG KTTDDASLIY RQAILETLKS
VNDNDIPEAL SNALEAVLSF AKNRFEALVP LFENLLMKRD QWAGRIMALD TIEMQEAVSS
VVLDAAQQAI SLIQGSDLER AMTCLKSASC ALDGFEWAAE MPAPIASAKA LEYFGQFASY
MLTSTGTLRA KVTVANGFPA KHFCTVEMNG ILADLKVNPH SQEYAQALAT LATLPELQYP
ARSAKMVEHF TVILRYLLAN LTLAMEGTNS LDFPEIAQRA IQALGAGETV GDALLEEDRI
LHIMVDEFQD TNQAQYDLLN LLVENWEPAD NRSIAYVGDG FQSIYLFRGA DLNLFTSVVE
AKAFGPKQMD ILRLTVNFRS LPGVVEWNNA TYAEVFKDSA YEFVPSVAFR KGDGGVSVHP
VATGQIGEAE EVTKVVQEAL TKDPSKSIAI LVRGRSHLKH ILPMLKEAGI EVRGKDIDPI
GEAAPVSEVI SLIRSLWHSA DRASWIALLR AAFVGLSWAD CLTVARGHNV IENALRDEGV
QAQLSSEGLT RVLRLLEVLD GVKRSSRGSE LGWAVKSVWI ALGGPATVNT GEMDDVETIF
KLLTSHTESG DLIDPQAFFR AIDKAYASPK AGAVTVMTCH GSKGLEYDIV LIPGLNKGGA
KDDAPLFYFR QMDGVFSVVP NLGDLDTTTP ESRLFKFVGR MVRKDISDEV CRAAYVGTTR
AKEDCHLFVC VDRFPAAEGE VQKEKEIKPA AGSLAECLWS AIGAQVKAVA PAFPIVAVTD
SGVPSKARLA PSYKVELPKT VFVPAASNDQ VPTENELNDE LREEEGSDYR AKTIGIVYHW
FVEQIGKQGI ELWDEARVRT KAQAIASMLR REGFPAAEVP QAVARILRLL SNTVNSIHGQ
WILKKRPGSG HEVQVSAYRN GRWVHRFLDR PFEDDGVYWI SDYKTPDCPE GMSEDAFVAR
EVERYRPKMD EYERAVKDAG ITIPVKKVLY FPAFDRLAEV A
//
