ID DDL_CALS8 Reviewed; 364 AA.
AC A4XJ89;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 29-MAY-2007, sequence version 1.
DT 29-MAY-2013, entry version 49.
DE RecName: Full=D-alanine--D-alanine ligase;
DE EC=6.3.2.4;
DE AltName: Full=D-Ala-D-Ala ligase;
DE AltName: Full=D-alanylalanine synthetase;
GN Name=ddl; OrderedLocusNames=Csac_1372;
OS Caldicellulosiruptor saccharolyticus (strain ATCC 43494 / DSM 8903).
OC Bacteria; Firmicutes; Clostridia; Thermoanaerobacterales;
OC Thermoanaerobacterales Family III. Incertae Sedis;
OC Caldicellulosiruptor.
OX NCBI_TaxID=351627;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 43494 / DSM 8903;
RA Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H.,
RA Pitluck S., Kiss H., Brettin T., Bruce D., Han C., Schmutz J.,
RA Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A.,
RA van de Werken H.J.G., Verhaart M.R.A., VanFossen A.L., Lewis D.L.,
RA Nichols J.D., Goorissen H.P., van Niel E.W.J., Stams F.J.M.,
RA Willquist K.U., Ward D.E., van der Oost J., Kelly R.M., Kengen S.M.W.,
RA Richardson P.;
RT "Genome sequence of the thermophilic hydrogen-producing bacterium
RT Caldicellulosiruptor saccharolyticus DSM 8903.";
RL Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Cell wall formation (By similarity).
CC -!- CATALYTIC ACTIVITY: ATP + 2 D-alanine = ADP + phosphate + D-
CC alanyl-D-alanine.
CC -!- COFACTOR: Binds 2 magnesium or manganese ions per subunit (By
CC similarity).
CC -!- PATHWAY: Cell wall biogenesis; peptidoglycan biosynthesis.
CC -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC -!- SIMILARITY: Belongs to the D-alanine--D-alanine ligase family.
CC -!- SIMILARITY: Contains 1 ATP-grasp domain.
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DR EMBL; CP000679; ABP66974.1; -; Genomic_DNA.
DR RefSeq; YP_001180165.1; NC_009437.1.
DR ProteinModelPortal; A4XJ89; -.
DR STRING; 351627.Csac_1372; -.
DR EnsemblBacteria; ABP66974; ABP66974; Csac_1372.
DR GeneID; 5087940; -.
DR KEGG; csc:Csac_1372; -.
DR PATRIC; 21252424; VBICalSac56748_1523.
DR eggNOG; COG1181; -.
DR HOGENOM; HOG000011593; -.
DR KO; K01921; -.
DR OMA; LLHGPFG; -.
DR ProtClustDB; PRK01966; -.
DR BioCyc; CSAC351627:GJ17-1406-MONOMER; -.
DR UniPathway; UPA00219; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:HAMAP.
DR GO; GO:0008716; F:D-alanine-D-alanine ligase activity; IEA:HAMAP.
DR GO; GO:0000287; F:magnesium ion binding; IEA:HAMAP.
DR GO; GO:0030145; F:manganese ion binding; IEA:HAMAP.
DR GO; GO:0009252; P:peptidoglycan biosynthetic process; IEA:HAMAP.
DR GO; GO:0008360; P:regulation of cell shape; IEA:UniProtKB-KW.
DR Gene3D; 3.30.1490.20; -; 1.
DR Gene3D; 3.30.470.20; -; 2.
DR Gene3D; 3.40.50.20; -; 1.
DR HAMAP; MF_00047; Dala_Dala_lig; 1; -.
DR InterPro; IPR011761; ATP-grasp.
DR InterPro; IPR013815; ATP_grasp_subdomain_1.
DR InterPro; IPR013816; ATP_grasp_subdomain_2.
DR InterPro; IPR000291; D-Ala_lig_Van_CS.
DR InterPro; IPR005905; D_ala_D_ala.
DR InterPro; IPR011095; Dala_Dala_lig_C.
DR InterPro; IPR011127; Dala_Dala_lig_N.
DR InterPro; IPR016185; PreATP-grasp_dom.
DR PANTHER; PTHR23132; PTHR23132; 1.
DR Pfam; PF07478; Dala_Dala_lig_C; 1.
DR Pfam; PF01820; Dala_Dala_lig_N; 1.
DR SUPFAM; SSF52440; PreATP-grasp-like; 1.
DR TIGRFAMs; TIGR01205; D_ala_D_alaTIGR; 1.
DR PROSITE; PS50975; ATP_GRASP; 1.
DR PROSITE; PS00843; DALA_DALA_LIGASE_1; 1.
DR PROSITE; PS00844; DALA_DALA_LIGASE_2; 1.
PE 3: Inferred from homology;
KW ATP-binding; Cell shape; Cell wall biogenesis/degradation;
KW Complete proteome; Cytoplasm; Ligase; Magnesium; Manganese;
KW Metal-binding; Nucleotide-binding; Peptidoglycan synthesis.
FT CHAIN 1 364 D-alanine--D-alanine ligase.
FT /FTId=PRO_1000030434.
FT DOMAIN 140 346 ATP-grasp.
FT NP_BIND 173 228 ATP (By similarity).
FT METAL 299 299 Magnesium or manganese 1 (By similarity).
FT METAL 313 313 Magnesium or manganese 1 (By similarity).
FT METAL 313 313 Magnesium or manganese 2 (By similarity).
FT METAL 315 315 Magnesium or manganese 2 (By similarity).
SQ SEQUENCE 364 AA; 40890 MW; 7AEAA0179641995C CRC64;
MTKLKVAVLF GGVSTEHEVS IVSAKSVMQN LDKEKYEIIP IGITKEGKWL LYTGKIEDLD
SKWTMYSIEC FISPDRTKKA LVKIKDSEAT FIDIDVVFPV LHGLNGEDGT VQGLLELSGI
PYVGCGVLSS ALCMDKAFAK KLALLEGIPQ GHFLVIYKDE YLSKKEYFIR RIESEFSYPV
FVKPANSGSS VGISKAKDKD ELILAIHEAF LYDTKILIEQ AINAREIECA VLGNSEILVS
ALGEIVPSRE FYSYEAKYID GSSELIIPAK LEKQVEDEIK ELAVKIYKLF ECSGMARIDF
FVDKETNKVY FNEVNTIPGF TSISMYPKLM EYSGIPYPQL LDKLIQLAIE KNQQKQSIKY
SKEG
//
