ID ISPD_PSEMY Reviewed; 235 AA.
AC A4XWR9;
DT 22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT 29-MAY-2007, sequence version 1.
DT 01-MAY-2013, entry version 45.
DE RecName: Full=2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase;
DE EC=2.7.7.60;
DE AltName: Full=4-diphosphocytidyl-2C-methyl-D-erythritol synthase;
DE AltName: Full=MEP cytidylyltransferase;
DE Short=MCT;
GN Name=ispD; OrderedLocusNames=Pmen_3031;
OS Pseudomonas mendocina (strain ymp).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=399739;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ymp;
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T.,
RA Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Detter J.C.,
RA Bruce D., Han C., Schmutz J., Larimer F., Land M., Hauser L.,
RA Kyrpides N., Mikhailova N., Hersman L., Dubois J., Maurice P.,
RA Richardson P.;
RT "Complete sequence of Pseudomonas mendocina ymp.";
RL Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Catalyzes the formation of 4-diphosphocytidyl-2-C-
CC methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-
CC phosphate (MEP) (By similarity).
CC -!- CATALYTIC ACTIVITY: CTP + 2-C-methyl-D-erythritol 4-phosphate =
CC diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol.
CC -!- PATHWAY: Isoprenoid biosynthesis; isopentenyl diphosphate
CC biosynthesis via DXP pathway; isopentenyl diphosphate from 1-
CC deoxy-D-xylulose 5-phosphate: step 2/6.
CC -!- SIMILARITY: Belongs to the IspD family.
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DR EMBL; CP000680; ABP85785.1; -; Genomic_DNA.
DR RefSeq; YP_001188517.1; NC_009439.1.
DR ProteinModelPortal; A4XWR9; -.
DR STRING; 399739.Pmen_3031; -.
DR EnsemblBacteria; ABP85785; ABP85785; Pmen_3031.
DR GeneID; 5107511; -.
DR KEGG; pmy:Pmen_3031; -.
DR PATRIC; 19912674; VBIPseMen131592_3077.
DR eggNOG; COG1211; -.
DR HOGENOM; HOG000218564; -.
DR KO; K00991; -.
DR OMA; FPQGAIL; -.
DR ProtClustDB; PRK00155; -.
DR BioCyc; PMEN399739:GHR6-3111-MONOMER; -.
DR UniPathway; UPA00056; UER00093.
DR GO; GO:0050518; F:2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity; IEA:HAMAP.
DR GO; GO:0019288; P:isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway; IEA:UniProtKB-UniPathway.
DR GO; GO:0016114; P:terpenoid biosynthetic process; IEA:HAMAP.
DR HAMAP; MF_00108; IspD; 1; -.
DR InterPro; IPR001228; ISPD_synthase.
DR InterPro; IPR018294; ISPD_synthase_CS.
DR Pfam; PF01128; IspD; 1.
DR TIGRFAMs; TIGR00453; ispD; 1.
DR PROSITE; PS01295; ISPD; 1.
PE 3: Inferred from homology;
KW Complete proteome; Isoprene biosynthesis; Nucleotidyltransferase;
KW Transferase.
FT CHAIN 1 235 2-C-methyl-D-erythritol 4-phosphate
FT cytidylyltransferase.
FT /FTId=PRO_1000202896.
FT SITE 17 17 Transition state stabilizer (By
FT similarity).
FT SITE 24 24 Transition state stabilizer (By
FT similarity).
FT SITE 158 158 Positions MEP for the nucleophilic attack
FT (By similarity).
FT SITE 214 214 Positions MEP for the nucleophilic attack
FT (By similarity).
SQ SEQUENCE 235 AA; 25629 MW; A07F6FADA7F51FB9 CRC64;
MSTKFWLVVP AAGVGARMAA DRPKQYLQVG GRCIIEHTLD CFLDHPDLLG AVVCLAVDDP
YWPQLAVASD PRVRRAPGGR ERADSVLAGL DALQAAGAGE QDWVLVHDAA RPNLAREDLQ
RLLAVLADDP VGGLLAVPVR DTLKRADADG RVAQTVDRSQ IWQAYTPQMF RLGALSQALR
GALAAGVPIT DEASALEWCG QSSRLVEGRA DNLKITRPED LAWLRQAWVE RDRQR
//
