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Database: UniProt
Entry: A4Y2M0
LinkDB: A4Y2M0
Original site: A4Y2M0 
ID   LEU1_SHEPC              Reviewed;         522 AA.
AC   A4Y2M0;
DT   28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   29-MAY-2007, sequence version 1.
DT   14-MAY-2014, entry version 52.
DE   RecName: Full=2-isopropylmalate synthase;
DE            EC=2.3.3.13;
DE   AltName: Full=Alpha-IPM synthase;
DE   AltName: Full=Alpha-isopropylmalate synthase;
GN   Name=leuA; OrderedLocusNames=Sputcn32_0471;
OS   Shewanella putrefaciens (strain CN-32 / ATCC BAA-453).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales;
OC   Shewanellaceae; Shewanella.
OX   NCBI_TaxID=319224;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CN-32 / ATCC BAA-453;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H.,
RA   Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J.,
RA   Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N.,
RA   Romine M.F., Fredrickson J., Tiedje J., Richardson P.;
RT   "Complete sequence of Shewanella putrefaciens CN-32.";
RL   Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the condensation of the acetyl group of
CC       acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form
CC       3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) (By
CC       similarity).
CC   -!- CATALYTIC ACTIVITY: Acetyl-CoA + 3-methyl-2-oxobutanoate + H(2)O =
CC       (2S)-2-isopropylmalate + CoA.
CC   -!- PATHWAY: Amino-acid biosynthesis; L-leucine biosynthesis; L-
CC       leucine from 3-methyl-2-oxobutanoate: step 1/4.
CC   -!- SUBUNIT: Homotetramer (By similarity).
CC   -!- SIMILARITY: Belongs to the alpha-IPM synthase/homocitrate synthase
CC       family. LeuA type 1 subfamily.
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DR   EMBL; CP000681; ABP74203.1; -; Genomic_DNA.
DR   RefSeq; YP_001182002.1; NC_009438.1.
DR   ProteinModelPortal; A4Y2M0; -.
DR   STRING; 319224.Sputcn32_0471; -.
DR   EnsemblBacteria; ABP74203; ABP74203; Sputcn32_0471.
DR   GeneID; 5080726; -.
DR   KEGG; spc:Sputcn32_0471; -.
DR   PATRIC; 23549494; VBIShePut135485_0476.
DR   eggNOG; COG0119; -.
DR   HOGENOM; HOG000046859; -.
DR   KO; K01649; -.
DR   OMA; EGQNALG; -.
DR   OrthoDB; EOG6CGCF3; -.
DR   BioCyc; SPUT319224:GHAP-479-MONOMER; -.
DR   UniPathway; UPA00048; UER00070.
DR   GO; GO:0003852; F:2-isopropylmalate synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009098; P:leucine biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.20.20.70; -; 1.
DR   HAMAP; MF_01025; LeuA_type1; 1.
DR   InterPro; IPR013709; 2-isopropylmalate_synth_dimer.
DR   InterPro; IPR002034; AIPM/Hcit_synth_CS.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR005671; LeuA_bact_synth.
DR   InterPro; IPR000891; PYR_CT.
DR   Pfam; PF00682; HMGL-like; 1.
DR   Pfam; PF08502; LeuA_dimer; 1.
DR   SMART; SM00917; LeuA_dimer; 1.
DR   SUPFAM; SSF110921; SSF110921; 1.
DR   TIGRFAMs; TIGR00973; leuA_bact; 1.
DR   PROSITE; PS00815; AIPM_HOMOCIT_SYNTH_1; 1.
DR   PROSITE; PS00816; AIPM_HOMOCIT_SYNTH_2; 1.
DR   PROSITE; PS50991; PYR_CT; 1.
PE   3: Inferred from homology;
KW   Amino-acid biosynthesis; Branched-chain amino acid biosynthesis;
KW   Complete proteome; Leucine biosynthesis; Transferase.
FT   CHAIN         1    522       2-isopropylmalate synthase.
FT                                /FTId=PRO_1000149287.
SQ   SEQUENCE   522 AA;  56939 MW;  BD179AC4101C6F53 CRC64;
     MSNRVIIFDT TLRDGEQALA ASLSVKEKLQ IAMALERLGV DVMEVGFPVS SPGDFESVQT
     IARTIKNSRV CALSRALEKD IDAAAQALSV ADQFRIHTFI STSTIHVESK LKRSFDQVLE
     MAVGAVKYAR RFTDDVEFSC EDAGRTPIDN LCRMVEAAIL AGARTINIPD TVGYTVPSEF
     GNIIQTLFNR VPNIDQAVIS VHCHDDLGLS VANSITAVQH GARQIECTIN GIGERAGNCS
     LEEIAMILAT RKGMLGLETG INAKEIHRTS NLVSQLCNMP VQANKAIVGA NAFTHSSGIH
     QDGMLKAQNT YEIMTPESIG LNRNNLNMTS RSGRHVIKHR MEEMGYSEHD YNMDTLYEEF
     LKLADKKGQV FDYDLEALAF MEAQAEEDNH YQLQQLVVQS DSTEGVATAT VRIEVGGEIK
     TEAATGNGPV DAAYNAIARA TDRRIDIISY KLGAKGVGQN ALGQVDITAV YHEQNFHGVG
     LATDVVEASA RALVHVMNLT CRADKVADYK QSMQKNRELG GV
//
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