ID   A4YY30_BRASO            Unreviewed;       543 AA.
AC   A4YY30;
DT   29-MAY-2007, integrated into UniProtKB/TrEMBL.
DT   29-MAY-2007, sequence version 1.
DT   27-MAR-2024, entry version 65.
DE   RecName: Full=cyclic-guanylate-specific phosphodiesterase {ECO:0000256|ARBA:ARBA00012282};
DE            EC=3.1.4.52 {ECO:0000256|ARBA:ARBA00012282};
GN   OrderedLocusNames=BRADO5111 {ECO:0000313|EMBL:CAL78806.1};
OS   Bradyrhizobium sp. (strain ORS 278).
OC   Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
OC   Nitrobacteraceae; Bradyrhizobium.
OX   NCBI_TaxID=114615 {ECO:0000313|EMBL:CAL78806.1, ECO:0000313|Proteomes:UP000001994};
RN   [1] {ECO:0000313|EMBL:CAL78806.1, ECO:0000313|Proteomes:UP000001994}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ORS 278 {ECO:0000313|Proteomes:UP000001994};
RX   PubMed=17540897; DOI=10.1126/science.1139548;
RA   Giraud E., Moulin L., Vallenet D., Barbe V., Cytryn E., Avarre J.C.,
RA   Jaubert M., Simon D., Cartieaux F., Prin Y., Bena G., Hannibal L.,
RA   Fardoux J., Kojadinovic M., Vuillet L., Lajus A., Cruveiller S., Rouy Z.,
RA   Mangenot S., Segurens B., Dossat C., Franck W.L., Chang W.S., Saunders E.,
RA   Bruce D., Richardson P., Normand P., Dreyfus B., Pignol D., Stacey G.,
RA   Emerich D., Vermeglio A., Medigue C., Sadowsky M.;
RT   "Legumes symbioses: absence of nod genes in photosynthetic bradyrhizobia.";
RL   Science 316:1307-1312(2007).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=3',3'-c-di-GMP + H2O = 5'-phosphoguanylyl(3'->5')guanosine +
CC         H(+); Xref=Rhea:RHEA:24902, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:58754, ChEBI:CHEBI:58805; EC=3.1.4.52;
CC         Evidence={ECO:0000256|ARBA:ARBA00034290};
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-
CC       pass membrane protein {ECO:0000256|ARBA:ARBA00004141}.
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DR   EMBL; CU234118; CAL78806.1; -; Genomic_DNA.
DR   AlphaFoldDB; A4YY30; -.
DR   STRING; 114615.BRADO5111; -.
DR   KEGG; bra:BRADO5111; -.
DR   eggNOG; COG4943; Bacteria.
DR   HOGENOM; CLU_000445_131_3_5; -.
DR   Proteomes; UP000001994; Chromosome.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-KW.
DR   GO; GO:0071111; F:cyclic-guanylate-specific phosphodiesterase activity; IEA:UniProtKB-EC.
DR   CDD; cd01948; EAL; 1.
DR   Gene3D; 3.20.20.450; EAL domain; 1.
DR   InterPro; IPR024744; CSS-motif_dom.
DR   InterPro; IPR001633; EAL_dom.
DR   InterPro; IPR035919; EAL_sf.
DR   PANTHER; PTHR33121; CYCLIC DI-GMP PHOSPHODIESTERASE PDEF; 1.
DR   PANTHER; PTHR33121:SF78; CYCLIC DI-GMP PHOSPHODIESTERASE PDEI-RELATED; 1.
DR   Pfam; PF12792; CSS-motif; 1.
DR   Pfam; PF00563; EAL; 1.
DR   SMART; SM00052; EAL; 1.
DR   SUPFAM; SSF141868; EAL domain-like; 1.
DR   PROSITE; PS50883; EAL; 1.
PE   4: Predicted;
KW   c-di-GMP {ECO:0000256|ARBA:ARBA00022636};
KW   Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW   Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW   Reference proteome {ECO:0000313|Proteomes:UP000001994};
KW   Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW   Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW   ECO:0000256|SAM:Phobius}.
FT   TRANSMEM        20..45
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        250..272
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   DOMAIN          275..528
FT                   /note="EAL"
FT                   /evidence="ECO:0000259|PROSITE:PS50883"
SQ   SEQUENCE   543 AA;  59237 MW;  741F30BFCD948F18 CRC64;
     MLRGHTGDVH GTMALQRKQM LRVGAVAACV GLFLTGADLA MTALIGGQTA RQLGDLSTVA
     LRRSEAAIDS AGEILNRLVA KGPMDCSAIA LQSLRLNVYQ GTIIKDIRTV DRNGSVLCAA
     YPETLEFDNG WARQEEMFKS TDGELRLFRV EQFTGSAFGV LRDIDARRSL AAILRIDGNL
     LDVMPVGLRG ESYVTLTLPD GEQVAGYHPD ASAKPPAQTV AFTTASERYP LQAAIHVPPR
     SLSARRDMTY LLTMLAAGLL GGLFGLLLAS YLSRPVDPVA ELDRALGGNE FKPHVQPTFR
     LKTGDIVGCE ILARWEKPDG RLIQPMSFIP LAESSGRIET LTWQLLKTAL RELQQEMRRD
     KLFKLSVNIT PRHLLGDGFI DELRKVVATS SVSTRQVVLE VTEREALADL DKAMTVVKEL
     SELGFRIALD DVGIGHSGLS TIQRLGARIL KIDKFFVDAI TRDQSAVVVV QMLVALAHEL
     DMSVIAEGIE RQEQVDALIA CGVEEGQGYL VAPPMSLDRF KQFLDVRRAS ATVARVTKPE
     LAA
//