ID A4YY81_BRASO Unreviewed; 500 AA.
AC A4YY81;
DT 29-MAY-2007, integrated into UniProtKB/TrEMBL.
DT 29-MAY-2007, sequence version 1.
DT 24-JAN-2024, entry version 90.
DE SubName: Full=Putative bifunctional enzyme (Sugar kinase/cytidylyltransferase) {ECO:0000313|EMBL:CAL78857.1};
DE EC=2.7.-.- {ECO:0000313|EMBL:CAL78857.1};
GN OrderedLocusNames=BRADO5164 {ECO:0000313|EMBL:CAL78857.1};
OS Bradyrhizobium sp. (strain ORS 278).
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
OC Nitrobacteraceae; Bradyrhizobium.
OX NCBI_TaxID=114615 {ECO:0000313|EMBL:CAL78857.1, ECO:0000313|Proteomes:UP000001994};
RN [1] {ECO:0000313|EMBL:CAL78857.1, ECO:0000313|Proteomes:UP000001994}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ORS 278 {ECO:0000313|Proteomes:UP000001994};
RX PubMed=17540897; DOI=10.1126/science.1139548;
RA Giraud E., Moulin L., Vallenet D., Barbe V., Cytryn E., Avarre J.C.,
RA Jaubert M., Simon D., Cartieaux F., Prin Y., Bena G., Hannibal L.,
RA Fardoux J., Kojadinovic M., Vuillet L., Lajus A., Cruveiller S., Rouy Z.,
RA Mangenot S., Segurens B., Dossat C., Franck W.L., Chang W.S., Saunders E.,
RA Bruce D., Richardson P., Normand P., Dreyfus B., Pignol D., Stacey G.,
RA Emerich D., Vermeglio A., Medigue C., Sadowsky M.;
RT "Legumes symbioses: absence of nod genes in photosynthetic bradyrhizobia.";
RL Science 316:1307-1312(2007).
CC -!- FUNCTION: Catalyzes the ADP transfer from ATP to D-glycero-beta-D-
CC manno-heptose 1-phosphate, yielding ADP-D-glycero-beta-D-manno-heptose.
CC {ECO:0000256|ARBA:ARBA00003753}.
CC -!- FUNCTION: Catalyzes the phosphorylation of D-glycero-D-manno-heptose 7-
CC phosphate at the C-1 position to selectively form D-glycero-beta-D-
CC manno-heptose-1,7-bisphosphate. {ECO:0000256|ARBA:ARBA00002319}.
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DR EMBL; CU234118; CAL78857.1; -; Genomic_DNA.
DR AlphaFoldDB; A4YY81; -.
DR STRING; 114615.BRADO5164; -.
DR KEGG; bra:BRADO5164; -.
DR eggNOG; COG0615; Bacteria.
DR eggNOG; COG2870; Bacteria.
DR HOGENOM; CLU_534032_0_0_5; -.
DR OrthoDB; 9802794at2; -.
DR Proteomes; UP000001994; Chromosome.
DR GO; GO:0016301; F:kinase activity; IEA:UniProtKB-KW.
DR GO; GO:0016779; F:nucleotidyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0016773; F:phosphotransferase activity, alcohol group as acceptor; IEA:InterPro.
DR GO; GO:0009058; P:biosynthetic process; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:UniProtKB-KW.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR CDD; cd01172; RfaE_like; 1.
DR Gene3D; 3.40.1190.20; -; 1.
DR Gene3D; 3.40.50.620; HUPs; 1.
DR InterPro; IPR004821; Cyt_trans-like.
DR InterPro; IPR011611; PfkB_dom.
DR InterPro; IPR011913; RfaE_dom_I.
DR InterPro; IPR029056; Ribokinase-like.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR NCBIfam; TIGR00125; cyt_tran_rel; 1.
DR PANTHER; PTHR46969; BIFUNCTIONAL PROTEIN HLDE; 1.
DR PANTHER; PTHR46969:SF1; BIFUNCTIONAL PROTEIN HLDE; 1.
DR Pfam; PF01467; CTP_transf_like; 1.
DR Pfam; PF00294; PfkB; 1.
DR SUPFAM; SSF52374; Nucleotidylyl transferase; 1.
DR SUPFAM; SSF53613; Ribokinase-like; 1.
PE 4: Predicted;
KW Carbohydrate metabolism {ECO:0000256|ARBA:ARBA00023277};
KW Kinase {ECO:0000313|EMBL:CAL78857.1};
KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268};
KW Nucleotidyltransferase {ECO:0000313|EMBL:CAL78857.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000001994};
KW Transferase {ECO:0000313|EMBL:CAL78857.1}.
FT DOMAIN 29..126
FT /note="Cytidyltransferase-like"
FT /evidence="ECO:0000259|Pfam:PF01467"
FT DOMAIN 184..480
FT /note="Carbohydrate kinase PfkB"
FT /evidence="ECO:0000259|Pfam:PF00294"
SQ SEQUENCE 500 AA; 54153 MW; AE01E25986723578 CRC64;
MDHIIAVPVD AVCEKIRASC ESGQTIAFVS GNFNVVHPGH LRLLKFAAEQ ADVLIVGVNP
DSTPGVTLAQ DMRLENVRSI SFVHHAVRLE ASASDFISHL KPTVVVKGKE FEDRANAEQD
AVDAYGGRLI FSSGELRFAS LSLLERDANI DISTVRKPLE FPKRHGFEIS NLKDLLGKLS
GMRVAVIGDL IVDEYVTCDA VGMSQEDPTI VVTPLMTRTF VGGAGAVAAH AHGLGADVKF
FTLVGDDEAA RFARSTLEQH GVNFNAFTDQ SRPTTRKQRF RALNKTLLRV NHLRQHASDA
DLQRQMLTSV ERALKTCDLL LFSCFNYGCL PQDLVDAIAD RARAQGVMMA ADSQVSSQTG
DVSRFKGMTL LTPTEREARV ALNDFISGLA VISERLVERA RTKNLVITLG AEGALINTMA
EGIFTSDRLP AFNPSPKDVS GAGDSFFMAC SMGLRAGADI WQASYLGSLA AALQVSRVGN
LPLTTAELVR EIDETGFSQE
//
