ID A5AE71_VITVI Unreviewed; 960 AA.
AC A5AE71;
DT 12-JUN-2007, integrated into UniProtKB/TrEMBL.
DT 12-JUN-2007, sequence version 1.
DT 27-MAR-2024, entry version 74.
DE RecName: Full=DNA helicase {ECO:0000256|ARBA:ARBA00012551};
DE EC=3.6.4.12 {ECO:0000256|ARBA:ARBA00012551};
GN ORFNames=VITISV_016661 {ECO:0000313|EMBL:CAN82075.1};
OS Vitis vinifera (Grape).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; Vitales; Vitaceae; Viteae; Vitis.
OX NCBI_TaxID=29760 {ECO:0000313|EMBL:CAN82075.1};
RN [1] {ECO:0000313|EMBL:CAN82075.1}
RP NUCLEOTIDE SEQUENCE.
RA Velasco R., Zharkikh A., Troggio M., Cartwright D.A., Cestaro A., Pruss D.,
RA Pindo M., FitzGerald L.M., Vezzulli S., Reid J., Malacarne G., Iliev D.,
RA Coppola G., Wardell B., Micheletti D., Macalma T., Facci M., Mitchell J.T.,
RA Perazzolli M., Eldredge G., Gatto P., Oyzerski R., Moretto M., Gutin N.,
RA Stefanini M., Chen Y., Segala C., Davenport C., Dematte L., Mraz A.,
RA Battilana J., Stormo K., Costa F., Tao Q., Si-Ammour A., Harkins T.,
RA Lackey A., Perbost C., Taillon B., Stella A., Solovyev V., Fawcett J.A.,
RA Sterck L., Vandepoele K., Grando S.M., Toppo S., Moser C., Lanchbury J.,
RA Bogden R., Skolnick M., Sgaramella V., Bhatnagar S.K., Fontana P.,
RA Gutin A., Van de Peer Y., Salamini F., Viola R.;
RT "The first genome sequence of an elite grapevine cultivar (Pinot noir Vitis
RT vinifera L.): coping with a highly heterozygous genome.";
RL PLoS ONE 2:e1326-e1326(2007).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000256|ARBA:ARBA00001665};
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DR EMBL; AM424362; CAN82075.1; -; Genomic_DNA.
DR EnsemblPlants; Vitvi19g00487_t001; Vitvi19g00487_P001; Vitvi19g00487.
DR Gramene; Vitvi19g00487_t001; Vitvi19g00487_P001; Vitvi19g00487.
DR ExpressionAtlas; A5AE71; baseline and differential.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0003724; F:RNA helicase activity; IEA:InterPro.
DR GO; GO:0015074; P:DNA integration; IEA:InterPro.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 1.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR025724; GAG-pre-integrase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001584; Integrase_cat-core.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR InterPro; IPR013103; RVT_2.
DR PANTHER; PTHR42648:SF18; INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN; 1.
DR PANTHER; PTHR42648; TRANSPOSASE, PUTATIVE-RELATED; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF13976; gag_pre-integrs; 1.
DR Pfam; PF00665; rve; 1.
DR Pfam; PF07727; RVT_2; 1.
DR SMART; SM00487; DEXDc; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF53098; Ribonuclease H-like; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS50994; INTEGRASE; 1.
DR PROSITE; PS51195; Q_MOTIF; 1.
PE 4: Predicted;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Nucleus {ECO:0000256|ARBA:ARBA00023242}.
FT DOMAIN 70..98
FT /note="DEAD-box RNA helicase Q"
FT /evidence="ECO:0000259|PROSITE:PS51195"
FT DOMAIN 101..215
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 536..701
FT /note="Integrase catalytic"
FT /evidence="ECO:0000259|PROSITE:PS50994"
FT REGION 238..268
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 290..340
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 70..98
FT /note="Q motif"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00552"
FT COMPBIAS 324..340
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 960 AA; 105615 MW; F6E5084AD6624990 CRC64;
MRRPNSRPQK RQFVKQKRLT ELQEIELLES WIEFGKPDSG SNPLSLAPPP SNAPIGRIDG
DSFSPYAGCD RFDRLPLSQK TIDGLKKSEY VTMTEIQRAS LPHSLCGRDI LGAAKTGSGK
TLAFLIPVLE KLYRLRWGPE DGVGSIIISP TRELTGQLFD VLKSVGKYHS FSAGLLIGGR
KDVGMEKEHV NELNILVCTP GRLLQHMDET PNFDCSQLQK PLTAGEIFRR TFRRPIFPTP
TIPEGAPGGB LQSPPTFVKA PASKEDPRAI HAPFSGRRLH LTRWRVRGRE EFSGDAHPPP
TSLDADQPPY IPGSPIRALH SSDSPLPQPP SSSASQASIA SVAQPGNASA CLTHTSSLGP
WILXSGASDH LSGNKDLFSS ITTTSALPTV TLANGSQTVA KGIGLALPLP SLPLTFVLYT
PECPFNLISI SKLTRTLNCS ITFSDKFVTL QDRSTGKTIG IGCESQGLYH FTSDSSAAVC
ISTDAPLLIH NRLGHPSLSK FQKMVPRFST LSSLPCESCQ LGKHTRVSFP KRLNNRAKSP
FELVHTDVWG PCRTTSTLGF QYFVTFINDY SRCTWLFLMK NRAELFSIFQ KFYAEIQTQF
NISIRVLRSD NAREYFSAPF TSFMSHHGIL HQSSCAHTPQ QNGVAERKNR HLVETAHTIL
LHSKDKLSAK AMKCLFLGYS RLQKGYRCYS LETHQYFIST DVTFFEDSPF FSTTSESLPV
SEVLPIPIVS PPDAMPPRPL QVYHRRPRVV APLPFAEAPA DSLPIPSASP APALPSPNDL
PIAVRKGTRS TRNPHPIYNF LSYHRLSSPY SAFVSAISSV SLPKSTHKAL SHPGWRQAMV
DEMAALHSNG TWDLVVLPSG KSTVGCRWVY AVKVGPDGQV DRLKARLVAK GYTQVYGSDY
GDTFSPVAKI ASVRLLLSMA AMCSWPLYQL DIKNAFLHGD LAEEIYMEQP PGFVAQGSLV
//
