ID A5CG59_9INFA Unreviewed; 752 AA.
AC A5CG59;
DT 12-JUN-2007, integrated into UniProtKB/TrEMBL.
DT 12-JUN-2007, sequence version 1.
DT 24-JAN-2024, entry version 61.
DE RecName: Full=RNA-directed RNA polymerase catalytic subunit {ECO:0000256|ARBA:ARBA00020035, ECO:0000256|RuleBase:RU004330};
DE EC=2.7.7.48 {ECO:0000256|ARBA:ARBA00012494, ECO:0000256|RuleBase:RU004330};
DE Flags: Fragment;
GN Name=pb1 {ECO:0000313|EMBL:CAM59198.1};
OS Influenza A virus (A/hooded vulture/Burkina Faso/2/2006(H5N1)).
OC Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina;
OC Insthoviricetes; Articulavirales; Orthomyxoviridae; Alphainfluenzavirus;
OC Alphainfluenzavirus influenzae; Influenza A virus.
OX NCBI_TaxID=406790 {ECO:0000313|EMBL:CAM59198.1};
RN [1] {ECO:0000313|EMBL:CAM59198.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=A/hooded vulture/Burkina Faso/2/2006
RC {ECO:0000313|EMBL:CAM59198.1};
RX PubMed=17622635; DOI=10.1099/vir.0.82939-0;
RA Ducatez M.F., Olinger C.M., Owoade A.A., Tarnagda Z., Tahita M.C., Sow A.,
RA De Landtsheer S., Ammerlaan W., Ouedraogo J.B., Osterhaus A.D.M.E.,
RA Fouchier R.A.M., Muller C.P.;
RT "Molecular and antigenic evolution and geographical spread of H5N1 highly
RT pathogenic avian influenza viruses in western Africa.";
RL J. Gen. Virol. 88:2297-2306(2007).
RN [2] {ECO:0000313|EMBL:CAM59198.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=A/hooded vulture/Burkina Faso/2/2006
RC {ECO:0000313|EMBL:CAM59198.1};
RA Ducatez M.F., Olinger C.M., Owoade A.A., Tarnagda Z., Tahita M.C., Sow A.,
RA De Landtsheer S., Ammerlaan W., Ouedraogo J.B., Osterhaus A.D.,
RA Fouchier R.A., Muller C.P.;
RT "Molecular and antigenic evolution and geographic spread of H5N1 highly
RT pathogenic Avian Influenza viruses in Africa.";
RL Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: RNA-dependent RNA polymerase which is responsible for
CC replication and transcription of virus RNA segments. The transcription
CC of viral mRNAs occurs by a unique mechanism called cap-snatching. 5'
CC methylated caps of cellular mRNAs are cleaved after 10-13 nucleotides
CC by PA. In turn, these short capped RNAs are used as primers by PB1 for
CC transcription of viral mRNAs. During virus replication, PB1 initiates
CC RNA synthesis and copy vRNA into complementary RNA (cRNA) which in turn
CC serves as a template for the production of more vRNAs.
CC {ECO:0000256|ARBA:ARBA00002148}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.48; Evidence={ECO:0000256|RuleBase:RU004330};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}. Host
CC cytoplasm {ECO:0000256|ARBA:ARBA00004192}. Host nucleus
CC {ECO:0000256|ARBA:ARBA00004147}. Nucleus
CC {ECO:0000256|ARBA:ARBA00004123}.
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DR EMBL; AM503062; CAM59198.1; -; mRNA.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0003968; F:RNA-dependent RNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0039523; P:suppression by virus of host mRNA transcription via inhibition of RNA polymerase II activity; IEA:UniProtKB-KW.
DR GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR GO; GO:0019083; P:viral transcription; IEA:UniProtKB-KW.
DR Gene3D; 6.10.140.720; -; 1.
DR HAMAP; MF_04065; INFV_RDRP; 1.
DR InterPro; IPR007099; RNA-dir_pol_NSvirus.
DR InterPro; IPR001407; RNA_pol_PB1_influenza.
DR Pfam; PF00602; Flu_PB1; 1.
DR PIRSF; PIRSF000827; RdRPol_OMV; 1.
DR PROSITE; PS50525; RDRP_SSRNA_NEG_SEG; 1.
PE 2: Evidence at transcript level;
KW Eukaryotic host gene expression shutoff by virus
KW {ECO:0000256|ARBA:ARBA00023247};
KW Eukaryotic host transcription shutoff by virus
KW {ECO:0000256|ARBA:ARBA00022731};
KW Host cytoplasm {ECO:0000256|ARBA:ARBA00023200};
KW Host gene expression shutoff by virus {ECO:0000256|ARBA:ARBA00022995};
KW Host nucleus {ECO:0000256|ARBA:ARBA00022562};
KW Host-virus interaction {ECO:0000256|ARBA:ARBA00022581};
KW Inhibition of host RNA polymerase II by virus
KW {ECO:0000256|ARBA:ARBA00023103};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW RNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022484,
KW ECO:0000256|RuleBase:RU004330};
KW Transferase {ECO:0000256|ARBA:ARBA00022484, ECO:0000256|RuleBase:RU004330};
KW Viral RNA replication {ECO:0000256|ARBA:ARBA00022953};
KW Viral transcription {ECO:0000256|ARBA:ARBA00023314}.
FT DOMAIN 285..482
FT /note="RdRp catalytic"
FT /evidence="ECO:0000259|PROSITE:PS50525"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:CAM59198.1"
FT NON_TER 752
FT /evidence="ECO:0000313|EMBL:CAM59198.1"
SQ SEQUENCE 752 AA; 85807 MW; 7D5EC797C94B0EDE CRC64;
DVNPTLLFLK VPVQNAISTT FPYTGDPPYS HGTGTGYTMD TVNRTHQYSE KGKWTTNTET
GAPQLNPIDG PLPEDNEPSG YAQTDCVLEA MAFLEESHPG IFENSCLETM EIVQQTRVDK
LTQGRQTYDW TLNRNQPAAT ALANTIEIFR SNGLTANESG RLIDFLKDVM ESMDKEEMEI
TTHFQRKRRV RDNMTKKMVT QRTIGKKKQR LNKKSYLIRA LTLNTMTKDA ERGKLKRRAI
ATPGMQIRGF VYFVETLARS ICEKLEQSGL PVGGNEKKAK LANVVRKMMT NSQDTELSFT
ITGDNTKWNE NQNPRMFLAM ITYITRNQPE WFRNVLSIAP IMFSNKMARL GRGYMFESKS
MKLRTQIPAE MLANIDLKYF NELTKKKIEK IRPLLIDGTA SLSPGMMMGM FNMLSTVLGV
SILNLGQKRY TKTTYWWDGL QSSDDFALIV NAPNHEGIQA GVDRFYRTCK LVGINMSKKK
SYINRTGTFE FTSFFYRYGF VANFSMELPS FGVSGINESA DMSIGVTVIK NNMINNDLGP
ATAQMALQLF IKDYRYTYRC HRGDTQIQTR RSFELKKLWE QTRSKAGLLV SDGGPNLYNI
RNLHIPEVCL KWELMDEDYQ GRLCNPLNPF VSHKEIESVN NAVVMPAHGP AKSMEYDAVA
TTHSWIPKRN RSILNTSQRG ILEDEQMYQK CCNLFEKFFP SSSYRRPVGI SSMVEAMVSR
ARIDARIDFE SGRIKKEEFA EIMKICSTIE EL
//
