ID A5FIA6_FLAJ1 Unreviewed; 325 AA.
AC A5FIA6;
DT 12-JUN-2007, integrated into UniProtKB/TrEMBL.
DT 12-JUN-2007, sequence version 1.
DT 27-MAR-2024, entry version 74.
DE SubName: Full=Candidate beta-D-glycosidase/alpha-L-glycosidase Glycoside hydrolase family 43 {ECO:0000313|EMBL:ABQ05058.1};
GN OrderedLocusNames=Fjoh_2027 {ECO:0000313|EMBL:ABQ05058.1};
OS Flavobacterium johnsoniae (strain ATCC 17061 / DSM 2064 / JCM 8514 / NBRC
OS 14942 / NCIMB 11054 / UW101) (Cytophaga johnsonae).
OC Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
OC Flavobacteriaceae; Flavobacterium.
OX NCBI_TaxID=376686 {ECO:0000313|EMBL:ABQ05058.1, ECO:0000313|Proteomes:UP000006694};
RN [1] {ECO:0000313|EMBL:ABQ05058.1, ECO:0000313|Proteomes:UP000006694}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 17061 / DSM 2064 / JCM 8514 / NBRC 14942 / NCIMB 11054 /
RC UW101 {ECO:0000313|Proteomes:UP000006694};
RX PubMed=19717629; DOI=10.1128/AEM.01495-09;
RA McBride M.J., Xie G., Martens E.C., Lapidus A., Henrissat B., Rhodes R.G.,
RA Goltsman E., Wang W., Xu J., Hunnicutt D.W., Staroscik A.M., Hoover T.R.,
RA Cheng Y.Q., Stein J.L.;
RT "Novel features of the polysaccharide-digesting gliding bacterium
RT Flavobacterium johnsoniae as revealed by genome sequence analysis.";
RL Appl. Environ. Microbiol. 75:6864-6875(2009).
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 43 family.
CC {ECO:0000256|ARBA:ARBA00009865, ECO:0000256|RuleBase:RU361187}.
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DR EMBL; CP000685; ABQ05058.1; -; Genomic_DNA.
DR RefSeq; WP_012024098.1; NZ_MUGZ01000007.1.
DR AlphaFoldDB; A5FIA6; -.
DR STRING; 376686.Fjoh_2027; -.
DR CAZy; GH43; Glycoside Hydrolase Family 43.
DR KEGG; fjo:Fjoh_2027; -.
DR eggNOG; COG3507; Bacteria.
DR HOGENOM; CLU_009397_4_0_10; -.
DR OrthoDB; 1016412at2; -.
DR Proteomes; UP000006694; Chromosome.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:UniProtKB-KW.
DR CDD; cd08991; GH43_HoAraf43-like; 1.
DR InterPro; IPR006710; Glyco_hydro_43.
DR InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR PANTHER; PTHR43772:SF2; BETA-1,4-XYLOSIDASE (EUROFUNG); 1.
DR PANTHER; PTHR43772; ENDO-1,4-BETA-XYLANASE; 1.
DR Pfam; PF04616; Glyco_hydro_43; 1.
DR SUPFAM; SSF75005; Arabinanase/levansucrase/invertase; 1.
DR PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism {ECO:0000256|ARBA:ARBA00023277};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU361187};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU361187};
KW Polysaccharide degradation {ECO:0000256|ARBA:ARBA00022651};
KW Xylan degradation {ECO:0000256|ARBA:ARBA00022651}.
FT ACT_SITE 38
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT ACT_SITE 211
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT SITE 148
FT /note="Important for catalytic activity, responsible for
FT pKa modulation of the active site Glu and correct
FT orientation of both the proton donor and substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-2"
SQ SEQUENCE 325 AA; 36346 MW; EDCD91CD37AFF419 CRC64;
MKKILSVITL FCVFTACSQE KSTDKPKEVK SNTILLADPT IFYNKGTYYL YGTTTGDFPN
GEGFQVYTST DLKDWKGPNG AQSGLALKKG DAFGDKGFWA PQILSYQNKF YMIYTANENI
AVAVSDSPLG PFKSESKEPI IKTGNQIDPF IFIDEDGKKY LYHVRLTNGN RIFAAEINDD
FQSIKPETLT ECISGILPWE NTQNVSWPVT EGPTVLKHNS LYYMIYSAND FRNIDYAVGY
ATSKSPLGPW EKAADSPIIS RKNTKQNGIG HGDVFWDKNN KMHYVLHTHF SESGVSPRKA
GIISIEFKDG KIVADPASFQ FLNVK
//
