GenomeNet

Database: UniProt
Entry: A5G7S3
LinkDB: A5G7S3
Original site: A5G7S3 
ID   TRMFO_GEOUR             Reviewed;         435 AA.
AC   A5G7S3;
DT   02-SEP-2008, integrated into UniProtKB/Swiss-Prot.
DT   12-JUN-2007, sequence version 1.
DT   26-NOV-2014, entry version 55.
DE   RecName: Full=Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO {ECO:0000255|HAMAP-Rule:MF_01037};
DE            EC=2.1.1.74 {ECO:0000255|HAMAP-Rule:MF_01037};
DE   AltName: Full=Folate-dependent tRNA (uracil-5-)-methyltransferase {ECO:0000255|HAMAP-Rule:MF_01037};
DE   AltName: Full=Folate-dependent tRNA(M-5-U54)-methyltransferase {ECO:0000255|HAMAP-Rule:MF_01037};
GN   Name=trmFO {ECO:0000255|HAMAP-Rule:MF_01037};
GN   OrderedLocusNames=Gura_3688;
OS   Geobacter uraniireducens (strain Rf4) (Geobacter uraniumreducens).
OC   Bacteria; Proteobacteria; Deltaproteobacteria; Desulfuromonadales;
OC   Geobacteraceae; Geobacter.
OX   NCBI_TaxID=351605;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Rf4;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H.,
RA   Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Schmutz J.,
RA   Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N.,
RA   Shelobolina E., Aklujkar M., Lovley D., Richardson P.;
RT   "Complete sequence of Geobacter uraniireducens Rf4.";
RL   Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the folate-dependent formation of 5-methyl-
CC       uridine at position 54 (M-5-U54) in all tRNAs. {ECO:0000255|HAMAP-
CC       Rule:MF_01037}.
CC   -!- CATALYTIC ACTIVITY: 5,10-methylenetetrahydrofolate + uracil(54) in
CC       tRNA + FADH(2) = tetrahydrofolate + 5-methyluracil(54) in tRNA +
CC       FAD. {ECO:0000255|HAMAP-Rule:MF_01037}.
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01037};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01037}.
CC   -!- SIMILARITY: Belongs to the MnmG family. TrmFO subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_01037}.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; CP000698; ABQ27841.1; -; Genomic_DNA.
DR   RefSeq; YP_001232414.1; NC_009483.1.
DR   ProteinModelPortal; A5G7S3; -.
DR   STRING; 351605.Gura_3688; -.
DR   EnsemblBacteria; ABQ27841; ABQ27841; Gura_3688.
DR   GeneID; 5165810; -.
DR   KEGG; gur:Gura_3688; -.
DR   PATRIC; 22037788; VBIGeoUra13052_3943.
DR   eggNOG; COG1206; -.
DR   HOGENOM; HOG000252054; -.
DR   KO; K04094; -.
DR   OMA; WEMRAAG; -.
DR   OrthoDB; EOG6J74VT; -.
DR   BioCyc; GURA351605:GI6A-3739-MONOMER; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-HAMAP.
DR   GO; GO:0030698; F:5,10-methylenetetrahydrofolate-dependent tRNA (m5U54) methyltransferase activity; IEA:UniProtKB-HAMAP.
DR   GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:UniProtKB-HAMAP.
DR   GO; GO:0047151; F:methylenetetrahydrofolate-tRNA-(uracil-5-)-methyltransferase (FADH2-oxidizing) activity; IEA:UniProtKB-EC.
DR   HAMAP; MF_01037; TrmFO; 1.
DR   InterPro; IPR004417; Folate-dep_Ribothymidyl_synth.
DR   InterPro; IPR002218; GIDA-rel.
DR   Pfam; PF01134; GIDA; 1.
DR   TIGRFAMs; TIGR00137; gid_trmFO; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Cytoplasm; FAD; Flavoprotein; Methyltransferase;
KW   Reference proteome; Transferase; tRNA processing.
FT   CHAIN         1    435       Methylenetetrahydrofolate--tRNA-(uracil-
FT                                5-)-methyltransferase TrmFO.
FT                                /FTId=PRO_0000346341.
FT   NP_BIND      10     15       FAD. {ECO:0000255|HAMAP-Rule:MF_01037}.
SQ   SEQUENCE   435 AA;  46993 MW;  071C10F2341B4083 CRC64;
     MIKEKLTIIG AGLAGCEAAW QAAGRGVAVT LHEMKPEKYS PAHHLPGLAE LVCSNSLRGE
     SLENAVGLLK EELRRLGSLF MEAALATRVP AGGALAVDRG LFSAFITDKI ENHPLIEVVH
     GEVAEIPADG TVIVASGPLT SDALAASIGK YTGDYLYFYD AIAPIVTSDS IDLSKAFRAS
     RYGKGDGDDY LNCPLDEAEY KAFIAALLAA EKVAAKEFEK VVHFEGCMPI EEMAERGLDT
     LRFGPMKPVG LIDPRTGIEP HAVVQLRQEN REGTLFNLVG FQTKLTYPEQ QRIFRTIPGL
     GKAEFVRLGS MHRNTFINAP QLLLSTFQLK REPRILFAGQ ITGVEGYVES AASGFLAGLN
     GARLAKGEAL IVPPSVTALG ALVNHITSAP AKHFQPMNIN YGLFPDLAGR VKKKEKRAKL
     AERALTELDG WMNTL
//
DBGET integrated database retrieval system