ID A5HZM6_CLOBH Unreviewed; 884 AA.
AC A5HZM6;
DT 26-JUN-2007, integrated into UniProtKB/TrEMBL.
DT 26-JUN-2007, sequence version 1.
DT 27-MAR-2024, entry version 95.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN Name=pcrA {ECO:0000313|EMBL:CAL82235.1};
GN OrderedLocusNames=CBO0683 {ECO:0000313|EMBL:CAL82235.1};
OS Clostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A).
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Clostridiaceae;
OC Clostridium.
OX NCBI_TaxID=441771 {ECO:0000313|EMBL:CAL82235.1, ECO:0000313|Proteomes:UP000001986};
RN [1] {ECO:0000313|EMBL:CAL82235.1, ECO:0000313|Proteomes:UP000001986}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Hall / ATCC 3502 / NCTC 13319 / Type A [Sanger]
RC {ECO:0000313|Proteomes:UP000001986};
RX PubMed=17519437; DOI=10.1101/gr.6282807;
RA Sebaihia M., Peck M.W., Minton N.P., Thomson N.R., Holden M.T.G.,
RA Mitchell W.J., Carter A.T., Bentley S.D., Mason D.R., Crossman L.,
RA Paul C.J., Ivens A., Wells-Bennik M.H.J., Davis I.J., Cerdeno-Tarraga A.M.,
RA Churcher C., Quail M.A., Chillingworth T., Feltwell T., Fraser A.,
RA Goodhead I., Hance Z., Jagels K., Larke N., Maddison M., Moule S.,
RA Mungall K., Norbertczak H., Rabbinowitsch E., Sanders M., Simmonds M.,
RA White B., Whithead S., Parkhill J.;
RT "Genome sequence of a proteolytic (Group I) Clostridium botulinum strain
RT Hall A and comparative analysis of the clostridial genomes.";
RL Genome Res. 17:1082-1092(2007).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by
CC translocating in the 3'-5' direction.; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034617};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922}.
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DR EMBL; AM412317; CAL82235.1; -; Genomic_DNA.
DR RefSeq; YP_001253224.1; NC_009495.1.
DR AlphaFoldDB; A5HZM6; -.
DR GeneID; 5184938; -.
DR KEGG; cbo:CBO0683; -.
DR PATRIC; fig|413999.7.peg.679; -.
DR HOGENOM; CLU_004585_5_5_9; -.
DR Proteomes; UP000001986; Chromosome.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0033202; C:DNA helicase complex; IBA:GO_Central.
DR GO; GO:0043138; F:3'-5' DNA helicase activity; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0000725; P:recombinational repair; IBA:GO_Central.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Coiled coil {ECO:0000256|SAM:Coils};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Reference proteome {ECO:0000313|Proteomes:UP000001986}.
FT DOMAIN 7..293
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 294..561
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT COILED 725..832
FT /evidence="ECO:0000256|SAM:Coils"
FT BINDING 28..35
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 884 AA; 103715 MW; F3ED713CCAF39258 CRC64;
MHLDVISGLN DAQREAVTTT EGYIRVIAGA GSGKTKALTS RYIYLVHELG ISTANILCAT
FTNKAAREMK KRIRTMIGDN DTGLIGTFHG FCRRLLKEDI YTINYPDNFI VMDNEDMDLV
LRIVYEDANI SSKQFTFSKA KDMISKRKNK DFKYISYIIE TDNNELKKKF LTSKNVEDRI
FYGYLYQQKK NYNLDFNDLI IFTLHILETF PKVRKKWQQK LEYIMVDEFQ DVNIKQYRLV
SILSEYHKNL FVVGDPDQTV YSWRGANVNL ILNFDKEFKG TKTIMMNQNY RSSSNIIKAS
NSLISKNKQR IDKELIPIKH GNIPVVYKHS ENIYKEANWI INQIEQIIKG GNSYKNIAIL
YRAHYVSRSI EEALLKNKIP YTLYSGVEFY QRKEIKDILS YLRMVVFEDD LSFIRIINEP
KRNFGKKRME IIRKYAEDNN CSLYSSLKIN LEDKLIAKSK ADKFVDLIER YKKIYKEMRI
TDLLMELLNN SGYEAMLRES GEDERLENLA ELKNSISDYE NSAGEDTTLE DYLQEISLFT
NSDLKENKDT INMMTLHTAK GLEFPYVFIC EFNEGIFPSA RTNTKEKMEE ERRLAYVGYT
RAKNALFLSD AEGVNYDGSY RFPSRFIFNT GKTYLNYLVE LKESLIDDAN EFISDSENSM
NKSNNIKFKI GERIIHKLLG LGVIIGIDND NSSYMIKFDN AETFRNISFS TQLDKADTNN
LDIIKSNERK AIDHLEAERL EAERKEQERL EAERLEAERK EQERLEAERL EAERKEQERL
EAERLEAERK EQERLEAERL EAERKEQERL EAERLEAERK EQERLEAEQI EVSTMILKIK
EEISELEEIV SKNKYKLFGK GAILKKEAKK RIQILYLKID EISN
//
