ID A5I578_CLOBH Unreviewed; 408 AA.
AC A5I578; A7G6E3;
DT 26-JUN-2007, integrated into UniProtKB/TrEMBL.
DT 26-JUN-2007, sequence version 1.
DT 27-MAR-2024, entry version 112.
DE RecName: Full=Flagellar biosynthesis protein FlhF {ECO:0000256|ARBA:ARBA00014919};
DE AltName: Full=Flagella-associated GTP-binding protein {ECO:0000256|ARBA:ARBA00030866};
GN Name=flhF {ECO:0000313|EMBL:CAL84205.1};
GN OrderedLocusNames=CBO2644 {ECO:0000313|EMBL:CAL84205.1};
OS Clostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A).
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Clostridiaceae;
OC Clostridium.
OX NCBI_TaxID=441771 {ECO:0000313|EMBL:CAL84205.1, ECO:0000313|Proteomes:UP000001986};
RN [1] {ECO:0000313|EMBL:CAL84205.1, ECO:0000313|Proteomes:UP000001986}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Hall / ATCC 3502 / NCTC 13319 / Type A [Sanger]
RC {ECO:0000313|Proteomes:UP000001986};
RX PubMed=17519437; DOI=10.1101/gr.6282807;
RA Sebaihia M., Peck M.W., Minton N.P., Thomson N.R., Holden M.T.G.,
RA Mitchell W.J., Carter A.T., Bentley S.D., Mason D.R., Crossman L.,
RA Paul C.J., Ivens A., Wells-Bennik M.H.J., Davis I.J., Cerdeno-Tarraga A.M.,
RA Churcher C., Quail M.A., Chillingworth T., Feltwell T., Fraser A.,
RA Goodhead I., Hance Z., Jagels K., Larke N., Maddison M., Moule S.,
RA Mungall K., Norbertczak H., Rabbinowitsch E., Sanders M., Simmonds M.,
RA White B., Whithead S., Parkhill J.;
RT "Genome sequence of a proteolytic (Group I) Clostridium botulinum strain
RT Hall A and comparative analysis of the clostridial genomes.";
RL Genome Res. 17:1082-1092(2007).
CC -!- FUNCTION: Necessary for flagellar biosynthesis. May be involved in
CC translocation of the flagellum. {ECO:0000256|ARBA:ARBA00025337}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|ARBA:ARBA00004413};
CC Peripheral membrane protein {ECO:0000256|ARBA:ARBA00004413};
CC Cytoplasmic side {ECO:0000256|ARBA:ARBA00004413}. Membrane
CC {ECO:0000256|ARBA:ARBA00004287}; Peripheral membrane protein
CC {ECO:0000256|ARBA:ARBA00004287}; Cytoplasmic side
CC {ECO:0000256|ARBA:ARBA00004287}.
CC -!- SIMILARITY: Belongs to the GTP-binding SRP family.
CC {ECO:0000256|ARBA:ARBA00008531}.
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DR EMBL; AM412317; CAL84205.1; -; Genomic_DNA.
DR RefSeq; WP_011986949.1; NC_009698.1.
DR RefSeq; YP_001255143.1; NC_009495.1.
DR RefSeq; YP_001388358.1; NC_009698.1.
DR AlphaFoldDB; A5I578; -.
DR GeneID; 5186899; -.
DR KEGG; cbh:CLC_2519; -.
DR KEGG; cbo:CBO2644; -.
DR PATRIC; fig|413999.7.peg.2628; -.
DR HOGENOM; CLU_009301_11_4_9; -.
DR Proteomes; UP000001986; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; IBA:GO_Central.
DR GO; GO:0005047; F:signal recognition particle binding; IBA:GO_Central.
DR GO; GO:0044781; P:bacterial-type flagellum organization; IEA:UniProtKB-KW.
DR GO; GO:0006605; P:protein targeting; IBA:GO_Central.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0006614; P:SRP-dependent cotranslational protein targeting to membrane; IEA:InterPro.
DR CDD; cd17873; FlhF; 1.
DR Gene3D; 1.20.120.1380; Flagellar FlhF biosynthesis protein, N domain; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR020006; FlhF.
DR InterPro; IPR047040; FlhF__GTPase_dom.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR000897; SRP54_GTPase_dom.
DR NCBIfam; TIGR03499; FlhF; 1.
DR PANTHER; PTHR43134:SF3; FLAGELLAR BIOSYNTHESIS PROTEIN FLHF; 1.
DR PANTHER; PTHR43134; SIGNAL RECOGNITION PARTICLE RECEPTOR SUBUNIT ALPHA; 1.
DR Pfam; PF00448; SRP54; 1.
DR SMART; SM00382; AAA; 1.
DR SMART; SM00962; SRP54; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
PE 3: Inferred from homology;
KW Bacterial flagellum biogenesis {ECO:0000256|ARBA:ARBA00022795};
KW Bacterial flagellum protein export {ECO:0000256|ARBA:ARBA00023225};
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW Cell projection {ECO:0000313|EMBL:CAL84205.1};
KW Cilium {ECO:0000313|EMBL:CAL84205.1};
KW Flagellum {ECO:0000313|EMBL:CAL84205.1};
KW GTP-binding {ECO:0000256|ARBA:ARBA00023134};
KW Membrane {ECO:0000256|ARBA:ARBA00023136};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Protein transport {ECO:0000256|ARBA:ARBA00022927};
KW Reference proteome {ECO:0000313|Proteomes:UP000001986};
KW Transport {ECO:0000256|ARBA:ARBA00022448}.
FT DOMAIN 211..366
FT /note="AAA+ ATPase"
FT /evidence="ECO:0000259|SMART:SM00382"
FT DOMAIN 212..403
FT /note="SRP54-type proteins GTP-binding"
FT /evidence="ECO:0000259|SMART:SM00962"
SQ SEQUENCE 408 AA; 46172 MW; 80D275961B106535 CRC64;
MKIKKYVVND MNEAMTRIRY ELGADAIIIS QRKIRKGGFL GLFSKKSLEV TAAIDNYSTE
KKYNSNTVNK INKGNNIEVI KRMLEERNNN VKYDNNINHD TNALDRYKEI LESKSEGAQN
KSLKGKKFSE SKDLMDEIRD LKKIVKDIGK SEKSYADENS SLVKFFKDLD LEEEFIEEII
KKVGNLEENL EEREKIKRVI EDTIDVRVNS VGKVTVLVGP TGVGKTTTIA KLAGKLSLID
KKKVGLITID TYRIGAVEQL KTYADIMNIP FKVVFSIKDM EKAIIDLDYC DVILVDTTGR
SSKNMMQISE LRAFIEKIKE KSVHLVISAS TKNKDIETIV KGYSILEYEN IIITKLDETS
IYGSILTILD KGKNPISFIT TGQDVPDDIK EGNKEEIAKI VLGENKLC
//
