ID   A5JW88_BORPT            Unreviewed;      1225 AA.
AC   A5JW88;
DT   26-JUN-2007, integrated into UniProtKB/TrEMBL.
DT   26-JUN-2007, sequence version 1.
DT   24-JAN-2024, entry version 54.
DE   SubName: Full=Bifunctional hemolysin-adenylate cyclase {ECO:0000313|EMBL:ABQ52218.1};
DE   Flags: Fragment;
GN   Name=cyaA {ECO:0000313|EMBL:ABQ52218.1};
OS   Bordetella pertussis.
OC   Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC   Alcaligenaceae; Bordetella.
OX   NCBI_TaxID=520 {ECO:0000313|EMBL:ABQ52218.1};
RN   [1] {ECO:0000313|EMBL:ABQ52218.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=DMST15659 {ECO:0000313|EMBL:ABQ52218.1};
RX   PubMed=17846749; DOI=10.1007/s00203-007-0302-1;
RA   Powthongchin B., Angsuthanasombat C.;
RT   "High level of soluble expression in Escherichia coli and characterisation
RT   of the CyaA pore-forming fragment from a Bordetella pertussis Thai clinical
RT   isolate.";
RL   Arch. Microbiol. 189:169-174(2008).
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000256|ARBA:ARBA00004613}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; EF595960; ABQ52218.1; -; Genomic_DNA.
DR   AlphaFoldDB; A5JW88; -.
DR   EMDB; EMD-26738; -.
DR   SMR; A5JW88; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR   GO; GO:0015267; F:channel activity; IEA:InterPro.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
DR   Gene3D; 2.150.10.10; Serralysin-like metalloprotease, C-terminal; 5.
DR   InterPro; IPR010566; Haemolys_ca-bd.
DR   InterPro; IPR018511; Hemolysin-typ_Ca-bd_CS.
DR   InterPro; IPR001343; Hemolysn_Ca-bd.
DR   InterPro; IPR018504; RTX_pore_form.
DR   InterPro; IPR003995; RTX_toxin_determinant-A.
DR   InterPro; IPR011049; Serralysin-like_metalloprot_C.
DR   PANTHER; PTHR38340; S-LAYER PROTEIN; 1.
DR   PANTHER; PTHR38340:SF1; S-LAYER PROTEIN; 1.
DR   Pfam; PF06594; HCBP_related; 1.
DR   Pfam; PF00353; HemolysinCabind; 9.
DR   Pfam; PF02382; RTX; 1.
DR   PRINTS; PR00313; CABNDNGRPT.
DR   PRINTS; PR01488; RTXTOXINA.
DR   SUPFAM; SSF51120; beta-Roll; 5.
DR   PROSITE; PS00330; HEMOLYSIN_CALCIUM; 5.
PE   4: Predicted;
KW   Cytolysis {ECO:0000256|ARBA:ARBA00022852};
KW   Membrane {ECO:0000256|ARBA:ARBA00023136};
KW   Secreted {ECO:0000256|ARBA:ARBA00022525};
KW   Toxin {ECO:0000256|ARBA:ARBA00022656};
KW   Virulence {ECO:0000256|ARBA:ARBA00023026}.
FT   DOMAIN          107..414
FT                   /note="RTX pore-forming"
FT                   /evidence="ECO:0000259|Pfam:PF02382"
FT   DOMAIN          1147..1175
FT                   /note="Haemolysin-type calcium binding-related"
FT                   /evidence="ECO:0000259|Pfam:PF06594"
FT   NON_TER         1
FT                   /evidence="ECO:0000313|EMBL:ABQ52218.1"
SQ   SEQUENCE   1225 AA;  126188 MW;  274DE0404F48DB3D CRC64;
     MTQFGRAGST NTPQEAASLS AAVFGLGEAS SAVAETVSGF FRGSSRWAGG FGVAGGAMAL
     GGGIAAAVGA GMSLTDDAPA GQKAAAGAEI ALQLTGGTVE LASSIALALA AARGVTSGLQ
     VAGASAGAAA GALAAALSPM EIYGLVQQSH YADQLDKLAQ ESSAYGYEGD ALLAQLYRDK
     TAAEGAVAGV SAVLSTVGAA VSIAAAASVV GAPVAVVTSL LTGALNGILR GVQQPIIEKL
     ANDYARKIDE LGGPQAYFEK NLQARHEQLA NSDGLRKMLA DLQAGWNASS VIGVQTTEIS
     KSALELAAIT GNADNLKSVD VFVDRFVQGE RVAGQPVVLD VAAGGIDIAS RKGERPALTF
     ITPLAAPGEE QRRRTKTGKS EFTTFVEIVG KQDRWRIRDG AADTTIDLAK VVSQLVDANG
     VLKHSIKLDV IGGDGDDVVL ANASRIHYDG GAGTNTVSYA ALGRQDSITV SADGERFNVR
     KQLNNANVYR EGVATQTTAY GKRTENVQYR HVELARVGQL VEVDTLEHVQ HIIGGAGNDS
     ITGNAHDNFL AGGSGDDRLD GGAGNDTLVG GEGQNTVIGG AGDDVFLQDL GVWSNQLDGG
     AGVDTVKYNV HQPSEERLER MGDTGIHADL QKGTVEKWPA LNLFSVDHVK NIENLHGSRL
     NDRIAGDDQD NELWGHDGND TIRGRGGDDI LRGGLGLDTL YGEDGNDIFL QDDETVSDDI
     DGGAGLDTVD YSAMIHPGRI VAPHEYGFGI EADLSREWVR KASALGVDYY DNVRNVENVI
     GTSMKDVLIG DAQANTLMGQ GGDDTVRGGD GDDLLFGGDG NDMLYGDAGN DTLYGGLGDD
     TLEGGAGNDW FGQTQAREHD VLRGGDGVDT VDYSQTGAHA GIAAGRIGLG ILADLGAGRV
     DKLGEAGSSA YDTVSGIENV VGTELADRIT GDAQANVLRG AGGADVLAGG EGDDVLLGGD
     GDDQLSGDAG RDRLYGEAGD DWFFQDAANA GNLLDGGDGR DTVDFSGPGR GLDAGAKGVF
     LSLGKGFASL MDEPETSNVL RNIENAVGSA RDDVLIGDAG ANVLNGLAGN DVLSGGAGDD
     VLLGDEGSDL LSGDAGNDDL FGGQGDDTYL FGVGYGHDTI YESGGGHDTI RINAGADQLW
     FARQGNDLEI RILGTDDALT VHDWYRDADH RVEIIHAANQ AVDQAGIEKL VEAMAQYPDP
     GAAAAAPPAA RVPDTLMQSL AVNWR
//