ID A5XA03_EMOCY Unreviewed; 268 AA.
AC A5XA03;
DT 10-JUL-2007, integrated into UniProtKB/TrEMBL.
DT 10-JUL-2007, sequence version 1.
DT 24-JAN-2024, entry version 46.
DE RecName: Full=V(D)J recombination-activating protein 1 {ECO:0000256|ARBA:ARBA00021277};
DE EC=2.3.2.27 {ECO:0000256|ARBA:ARBA00012483};
DE Flags: Fragment;
GN Name=Rag-1 {ECO:0000313|EMBL:ABI78926.1};
OS Emoia cyanura (Azure-tailed skink).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Lepidosauria; Squamata; Bifurcata; Unidentata; Scinciformata; Scincidae;
OC Eugongylinae; Emoia.
OX NCBI_TaxID=38244 {ECO:0000313|EMBL:ABI78926.1};
RN [1] {ECO:0000313|EMBL:ABI78926.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=17400482; DOI=10.1016/j.ympev.2007.02.007;
RA Smith S.A., Sadlier R.A., Bauer A.M., Austin C.C., Jackman T.;
RT "Molecular phylogeny of the scincid lizards of New Caledonia and adjacent
RT areas: evidence for a single origin of the endemic skinks of Tasmantis.";
RL Mol. Phylogenet. Evol. 43:1151-1166(2007).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.27; Evidence={ECO:0000256|ARBA:ARBA00000900};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946};
CC -!- COFACTOR:
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC Evidence={ECO:0000256|ARBA:ARBA00001936};
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DR EMBL; DQ675343; ABI78926.1; -; Genomic_DNA.
DR AlphaFoldDB; A5XA03; -.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-KW.
DR GO; GO:0004519; F:endonuclease activity; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; IEA:InterPro.
DR GO; GO:0033151; P:V(D)J recombination; IEA:InterPro.
DR Gene3D; 3.30.40.10; Zinc/RING finger domain, C3HC4 (zinc finger); 1.
DR InterPro; IPR024627; RAG1.
DR InterPro; IPR035714; RAG1_imp-bd.
DR InterPro; IPR018957; Znf_C3HC4_RING-type.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR InterPro; IPR017907; Znf_RING_CS.
DR PANTHER; PTHR11539:SF0; V(D)J RECOMBINATION-ACTIVATING PROTEIN 1; 1.
DR PANTHER; PTHR11539; VDJ RECOMBINATION ACTIVATING PROTEIN 1 RAG1; 1.
DR Pfam; PF12560; RAG1_imp_bd; 1.
DR Pfam; PF00097; zf-C3HC4; 1.
DR SUPFAM; SSF57850; RING/U-box; 1.
DR PROSITE; PS00518; ZF_RING_1; 1.
PE 4: Predicted;
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nucleus {ECO:0000256|ARBA:ARBA00023242};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Zinc {ECO:0000256|ARBA:ARBA00022833};
KW Zinc-finger {ECO:0000256|ARBA:ARBA00022771}.
FT DOMAIN 30..241
FT /note="RAG1 importin-binding"
FT /evidence="ECO:0000259|Pfam:PF12560"
FT DOMAIN 243..267
FT /note="Zinc finger C3HC4 RING-type"
FT /evidence="ECO:0000259|Pfam:PF00097"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:ABI78926.1"
FT NON_TER 268
FT /evidence="ECO:0000313|EMBL:ABI78926.1"
SQ SEQUENCE 268 AA; 30706 MW; E59C8E6A30B0F82C CRC64;
CSHVANHDKG RVEVDQVFEG KENGVDPFLR DKDQNNSVET DKDAFRMNQR EIEAHQAKLQ
HLCRICGGSF KADSCKRSHP VHGPVDNVMQ ALLRKKERRA TSWPELLAKV FKIDVRGDID
TIHPTKFCHN CWNFVQRKFS NVPSEVYFPR NDPREWHPHS SGCEVCGTLA RGVKRKRQVL
KPQMSKKLRI VARGGSQIRP ERGPQKVHSK NFMKKIVNCN KIHLSTKSFA VDYPADFVKA
VSCQICEHIL ADPVETTCKH LFCRVCIL
//
