ID A6N9L3_CLAPU Unreviewed; 6847 AA.
AC A6N9L3;
DT 24-JUL-2007, integrated into UniProtKB/TrEMBL.
DT 24-JUL-2007, sequence version 1.
DT 27-MAR-2024, entry version 74.
DE SubName: Full=Non-ribosomal peptide synthetase {ECO:0000313|EMBL:ABR23346.1};
OS Claviceps purpurea (Ergot fungus) (Sphacelia segetum).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Hypocreomycetidae; Hypocreales; Clavicipitaceae; Claviceps.
OX NCBI_TaxID=5111 {ECO:0000313|EMBL:ABR23346.1};
RN [1] {ECO:0000313|EMBL:ABR23346.1}
RP NUCLEOTIDE SEQUENCE.
RA Keller U., Ortel I., Riederer B.;
RT "Analysis of a peptide synthetase from the ergot fungus Claviceps
RT purpurea.";
RL Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- PATHWAY: Alkaloid biosynthesis; ergot alkaloid biosynthesis.
CC {ECO:0000256|ARBA:ARBA00005107}.
CC -!- SIMILARITY: Belongs to the NRP synthetase family.
CC {ECO:0000256|ARBA:ARBA00029454}.
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DR EMBL; EF633829; ABR23346.1; -; Genomic_DNA.
DR VEuPathDB; FungiDB:CPUR_06496; -.
DR UniPathway; UPA00327; -.
DR GO; GO:0016874; F:ligase activity; IEA:UniProtKB-KW.
DR GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR GO; GO:0044249; P:cellular biosynthetic process; IEA:UniProt.
DR GO; GO:0035835; P:indole alkaloid biosynthetic process; IEA:UniProtKB-UniPathway.
DR CDD; cd05918; A_NRPS_SidN3_like; 5.
DR CDD; cd19542; CT_NRPS-like; 1.
DR CDD; cd19545; FUM14_C_NRPS-like; 4.
DR Gene3D; 3.30.300.30; -; 6.
DR Gene3D; 1.10.1200.10; ACP-like; 6.
DR Gene3D; 3.30.559.10; Chloramphenicol acetyltransferase-like domain; 7.
DR Gene3D; 3.40.50.12780; N-terminal domain of ligase-like; 6.
DR Gene3D; 3.30.559.30; Nonribosomal peptide synthetase, condensation domain; 8.
DR InterPro; IPR010071; AA_adenyl_domain.
DR InterPro; IPR036736; ACP-like_sf.
DR InterPro; IPR045851; AMP-bd_C_sf.
DR InterPro; IPR020845; AMP-binding_CS.
DR InterPro; IPR000873; AMP-dep_Synth/Lig_com.
DR InterPro; IPR042099; ANL_N_sf.
DR InterPro; IPR023213; CAT-like_dom_sf.
DR InterPro; IPR001242; Condensatn.
DR InterPro; IPR020806; PKS_PP-bd.
DR InterPro; IPR009081; PP-bd_ACP.
DR InterPro; IPR006162; Ppantetheine_attach_site.
DR NCBIfam; TIGR01733; AA-adenyl-dom; 3.
DR PANTHER; PTHR45527; NONRIBOSOMAL PEPTIDE SYNTHETASE; 1.
DR PANTHER; PTHR45527:SF3; SIDEROPHORE SYNTHETASE (EUROFUNG)-RELATED; 1.
DR Pfam; PF00501; AMP-binding; 6.
DR Pfam; PF00668; Condensation; 7.
DR Pfam; PF00550; PP-binding; 6.
DR SMART; SM00823; PKS_PP; 5.
DR SUPFAM; SSF56801; Acetyl-CoA synthetase-like; 6.
DR SUPFAM; SSF47336; ACP-like; 6.
DR SUPFAM; SSF52777; CoA-dependent acyltransferases; 15.
DR PROSITE; PS00455; AMP_BINDING; 4.
DR PROSITE; PS50075; CARRIER; 6.
DR PROSITE; PS00012; PHOSPHOPANTETHEINE; 5.
PE 3: Inferred from homology;
KW Ligase {ECO:0000256|ARBA:ARBA00022598};
KW Phosphopantetheine {ECO:0000256|ARBA:ARBA00022450};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553}.
FT DOMAIN 101..177
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 1169..1245
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 2267..2343
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 4101..4177
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 5195..5271
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 6332..6408
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT REGION 6221..6252
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 6654..6684
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 6847 AA; 757199 MW; 13E4E97F8F62F5A6 CRC64;
MSQSGNASSI TPPLAELEDL VADMKAPMPE APRVLPIPAD IGDKLAEHLT AYMMPKILIT
MKKLPMTATG KLDRRRLREI GSSFSAQELA ELQMERDAKR EPRTILAKQM QQVWAKVLNI
QPEIIGLDDS FFRLGGDSIA AMKLVGEMRK HGVELAVADI FRYPTLDLAT KQGNTFNSLS
SIATEVIKPF SLLFQRTRYR LFSFKMSRSA SAEVSGSGGG CLPLALRFKK DCYALNETPQ
VTMSRKSVLE LAPGLDLEAF RRAWGEVARG IAILRTRVVQ HRGSVIQVVL DEKIDWHETT
NLISTWKPTN EINAFEPALV RYALVKDASG AFKWFVWTVH HALYDDWSLS LIIDAVHQAF
CGKSVDFGPQ FTRFIEYLSN QDYNKLVNYW HGALSSYTDT SFPALPPSIQ HPRADRTLQG
EIFLPRKQQS GITLSNLLRA AWALVAGDLT NSQDVVFGMT MSGRGAQVPG LNKMAAPTIV
TVPVRVTWSK DQKVSDYVNM VQNQATDMIP FEQAGLQKIA KICPGSHQAC QFQTLIVIQA
PGSDSISTYA LMLDLQLGLR QRYHIGHFDP NVIEEPTVKG YSRGSNLFSA ARYLDPKMQL
EEVNMMTEQD LQKIWHWNGV VPQTAERCVH ELVEEKIQSN PDASAICAWD GSLTYGQLGD
LVTILAGHLA HLGIGPGVIV PLCFEKSMWT TPPLLLFSRQ AVLHLTTDPL IPEQRLAAIA
QQIEMKLLIS STSNSALGSR LAHTTLVLCS DFFTSHLIRS SSTALHASPA DLMYVVFTSG
STGTPKGVKI AHKNLASAIH HQAEALGFNS SSRFYDFTSY SFDTCIANIF FTLASGGCLC
VPSDGDRKSN LGASVATFRA NLIELTPTVA QTLVPNDVPE IRSIAFGGEA YCRGRRANRL
YMTLSDGGER KVSNTYGPAE CTPTSKMNIH SLTPEETTRI GFGAGARTWI VNPDDYHQLL
PVGCVGELLL EGPIVGLGYL NDDEQTNTVF VKDPAWLQKG AGCLGRDGRL YRTGDLVRYN
TDGSLTFMGR KDDQVKLRGQ RIELNEVEAY IKKALSKSQR FMWLRTSLHR VNDDRPILVA
FISILRDSHD AEDEGSARVL LSELTEDLED TLAATLPSYM VPSVFLEAKQ MPTTPTGKID
RRALRVQAGC MTKDELVSQN ILRRRAYCAP DSVAEKQLQG LWSSVLGLEI SSIGSNDSFF
RIGGDSIQAM RLAGAAREQG FSLTVADTFK YPKLRDLAKM LSQNAVTSLD TPNPYSLLDS
AVDRIDAQSQ AATMCQVSAS QVVDILPCSP LQEGLLAMTL KRPGDYIYYN EYLLAEDVDA
LRFKMACEKV ITANPILRTR IRRFAAAGQC KLSLTNKFVG RMTCQRSLTR RQWALGTQLA
LVGLTTSAEN GRLIFHWILH HALYDAWSFS MMLDSIEAAY SGLQKEEKSV PFSSFVGHIL
AVNKEEAAEY WRTYLHEIDS STFPVLPALD HSPRPDAIHL ALDAIVQLDI DNITWPQSDI
TASTVIRTAW ALLQATNTGR DDVLYGATVA GRQRALPGID RVMGPTIATV PVRVSLDWNG
STVGALLLSV QSQFLEAVPF EQIGLQNIRK LSEDAEIACN FQTLLVTQPT SSDDDKSGRP
QQLFHNCRNS EGHDDLAIFN TYCITVSCDY SKFGVQIAIS YDTAIINYAE ANRLLLGLEH
FICELCKPGV ELKSLKDLTA VTDHDMRKLW EKNAVVPDSV HVCVHDLIAQ TIQRQPQAPA
VCAWDDMLTS HELTYGELDR LSTQLAHQLV RLGVAPGEII PLCFERSVWT SVAMLGVMKA
GGASVALEIT QPLERLRSIV QQVSPITILA SSTSESIAAQ LHDSVKVKIV SRGTCEVETD
GNLNELPSRA TPSDPLYVVF TSGTTGKPKG VVVSHASFSS ASVYQRKALG FTTGDRVLGF
ASPAFDLFWS NHLNTLITGC CIYTPTESQR TDNLAGFIAD QQITLAHLTP STARLLRSNL
SPTMSTLILG GELVSREDFD CFSPSVNVKI TYGPSECSPT STILDTNEFS WRTGNIGEPK
GLCAWVVHPV RHTLAAPGSV GELWLEGRLV ASGYLRDPEK TAAAFIEDPA WLLRGGPGVP
GRRGRVYRTG DLVRYDIDNG TIHFVGRKDS QVKIRGQRVE LAEVELHIRQ VLVHKPGVEV
AVDVITTRDS TKPILVAFIS LPSQEISARD GDKVQAGQKS AVLAVHGGLR RAIGQTYSCL
YDSICLSAHP YHSHDYNRQD RQQSLKRVGA ALSLAEIVTQ SQKKPTAPSS DIEVMLATIW
AQTLNLPYES ISVDVPFTKI GGDSISAMQV VARSRSMGLK LTVSDVLRHL TIEKIVPHGE
LLRSSRTTDD LHTEQDNQPW QLSPIQNMFF MAHPSGLHHF NQSFAFRLAS TVTVTHLRMA
AETLVSRHPM LRARFRLNIE GKWEQYTVPN DENAFTFKVH RLREDEDSGH LLRERQKTLH
IITGPVFAID CLLLGGDNHD NDDDSTTFDI IVLFTAHHLI IDLVSWRIIW YEMEQLVKGS
SNLPAPSTSF KSWVCKQATT ERVSDAGQLL PFQLTTNLPN GALHPSDNTI SASSDIHGCL
DSETTSLLLG KSNDSMRTDT VDIMVAVISH SLRQHFPERC APAIFLEGHG REPSDDTSVD
LSETIGWFTT ICPVQVPLSH DHDVVDAVKF VKDTRRRIPG KGLTYFNNRY GVASAENGAC
QAKFEPVEFL LNYAGIYQQI EREESIFSPL DVDLMELSSD TIRTALVEIN CGVRFGELHV
SVTMHNRMKH RDRLEQWAKS LGQQFQSALQ ALIKAGRVAT LVDYSLLRVS YKTLENLMAH
LGRMEINIDN VADIFPCTPL QEGILLSMSK TSSSYRIVNI WKCISGDGKV DVSRLESAWR
SAIARHSVFR IIFVESAGYQ GFLQVQLLRA PVKITVLRTD IDSSSPEHTL RSLEWPHFKE
TEPEYAITIC GTATGHVACR FDISHALIDA TSFAILLRDV REAYHNSSSA QAAPSFGHAV
EEIMKTPHSD RLVYWKKYLA GVKACRLPSI PQVADGCAEF QQGWLPIPLG TSSLLHSFCQ
AHCLTRSTVL YVAWALVLSQ LTGSRDICFG YLASGRDIPI KGVHDLVGPL INLLIGRINV
SLPIDDVLAK TNQDSIDHFS FQHVSLAELQ REIGMQGDQL FTTSITIRHD LDDNCAPDGD
SLGFDSVEGE DPHEVRLTYI SWRLLVQEFL YLLTLVYSSI LFLRRDETAP DTEMSLNYNM
SKISQSLVSE AADVFAAAIT YLTDIRNNCT QEPLDGAFFR YTTGVAESKA LSTWKGWMQD
LETSHFPSLP HPSYMSQRQV SETHYIGGIS TGRNAVQTIA ILWTSWALLL ATNSNSSDVV
FGSATPYEAM ENCNPENRQS ASTVPVRVKI DYNQTVGDKL NELGAICDAV TQYRRISAHW
LRRISEQGEQ FCAFQSALEI RFASSASTVQ HESQKEVDCM PLRLTCVVTE CSLQLTVHYD
DQVLNDIQAR RLYRQFEAIF RQLCNPKAAK MKLHSISVTP EQEMREIWQW NANVPRTARA
CVHDLIADTI RRQPHAQAIN AWDGELTYGE LDTLSSQLAS SLLETCQIRP GTIVTLCFEK
SMWTPVAMLG VMKAGGASVA MDVTQPSGRL REILRQSSST IILTSQASES LAQSLAVDLH
EQPPTILAIP KSFKIDRATV CQPPRTSVVP EDMLYVVFTS GSTGTPKGIA ISHANFSSAI
LHQQDVLCYQ PSSRVFDSAS YAFDVAWFNV LFTLKVGGCL CIPNENDRRG DISGAIRSLK
ANYVELTATV GRLIKAAEVP CLQTIQFCGE RLTLSALDQW HHVEELFHCY GPAECTAATT
VNRIMRVGDQ DPHIGKGCGT VTWVVSTRRY GLASIGEVGE LWVEGPLVAM GYLNNPEKTS
SSFIEDPVWL LQGGPGVPGR HGRLYRTGDL VRYDDDGNLH FIGRTDDQVK IRGQRVQLGE
IEAHIRTAMP STAPPDLQIT VEMVKLQDCS NPILVSFIHL PGPPKVLKND VKNGRPHKKH
SQEEDWHCFH AAASQMAQGL EEKLAGVLPS YMIPSACLVV DSMPMTATGK TDRRELRAIT
GRLTQKELAM QNVARSHKKL VPVTATEVKL RQVWSDVLDT STADISTDDS FLQIGGDSVQ
AMRLAGALRE QSLCVTVAEI FKHLRLRDLA KLLSERGSTP NAIASPASFS LLRNTVDYNS
ASAKVAALCG VDTSQIQDIF PCTPLQQGLL AITTRRSGDF YVSHNEFVLG QDIERPKFQS
ACHKAVKLLP ILRTRIVDLP GQGLVQAVID EDAAWYDRSI TTSEIKMGLG TRLAIFGLVS
SADTGRLVFH LILHHSLFDG WSYPLMLSFI QDIYLDKQPM KVVPFQNFVQ YTLGCDAHAA
SLHWQSCFDG ITAVMFPALP SPDYSPNVDG SLEHMIEDCT MAAVGYNRST VVQTAWALVQ
SVHTATDDVV FGAVVTGRQA LPGVDRIIGP TLATIPCRVT LNWQATISDF LQQMQQSLDD
ASFQQTGLQN IRKASADAGD GLRLPDIAHH PACAARKQSG SFFELRDDPC SENSTFDTYA
SWSKSFWKPT AYYWSLKYDS ALWVLHMSGV SQSIRKHFTS IEHLRQLVRL SHDDALTMRD
IQVVTEECTR KIWKWNATVP QPIDTCVHDL IAETVRKQPR ALAVSSWDGN LLYSELEELS
YTISKSSHKT GVRPGLIIPL YFEKSKWLIA MLGVMKAGGA SVAIDISQPL ERLREIARQV
APTIILTSAT GASLAGKHTC QWFDHCRHCG HFRLRTTQVK DTRTLQETRL DPLESQRELR
SLTQISPQRA IIKAKASGLT AVRCARTFLF CVYVFWSTSL NSFISGACLC IPTESQRKDD
LSGFMNQQKV TLADLTPSTA KVIRTCPPKY LKTLILGGEL VSLQEFESLF SSSINIKITY
GPAECTPTSS ILDTAFGSFE TADIGHCWGL CAWVAHPTSH NLIPPGLVGE LWLEGPLVGM
TYLNDNDKTK ASFVDDPSWL LRGVGPEELG RRHGRLYRTG DLVRYNEDGS LHFIVRKDDQ
VKIRGQRVEL DEVEMHIRGA LGSGNSSSAT DFEIVADVVT PRGSKIPILV SFISVQNASE
SFVQEMDREV RDGIGERKDG EKLRVNIERL LEGLDEKLTA VIPSYMIPSV YLEVGRIPMT
TSGKTDRRKL RATASGLSLE ELTSKNLLHR CKHREPTTDR EKRMRRIWAE VLEIDETSIS
ADDSFLQIGG DSIQAMRLTG IARERGFLLT VAGIFKTPSF LSLAKSTVAS DMQRFVDVPE
PFSLLSTQVS TRVDKEEARL QISSLCGVST DQVEDILPCI PLQEGMLAMT AKGSGNYVAY
YEYILAKSVD ASRFRNACQA VVSALPILRT RIADLPGHGL VQAVISEKIC WGTTSGANFP
ATSPPTTMEP GSPLVLFELS TSDSGRLIFR WTLHHALFDG WTLPLMLQAI EDAYYDKFLP
KNFLPFSTFV GYVQDTDNEA LVSWWLLTWM ESARQYSLRF LSQTTHRGRM AHLEETIKVN
WPQSDITPST IVRTAWALLQ AMYTSSDDVI FGATVTGQHA AIPGIESIVG PTIATVPYEL
LSTGAKTLEN CFMRCNNRSL DVTPFEQVGL QRLRKISHDA EMACNFQTCL WYNRRNSHED
SVEPFFESLE SRAGSTSSRV CGWVKYLCSY GTMRPQRRRF QHDAKLRLLY YRHPSDRTIH
ESVAPSCRAN VQDWTKWASL SDVVTIARED LLRVWQWNKT VPAPIDSCVH DLIADAARKH
PQAPAVCAWD GELTYEKLDR LSTQVAHRLS KLGVGRGTVV PLCFEKSMWM PVAALGVMKA
GGASVALDTT QPLDRLRNIV NQVEGPSSNM GCQLILASSL HEALSNKLMR HGKQNKTLVL
SDLTLREDHE TNPKDLPASG VVPADSLYVV FTSGSTGHPK GVVITHSNFS SAIIHQRSSL
DMQQSSRVFD FASYAFDAAW MNILHTLAAG ACLCIPSEED RRSDVSGAIQ KLRANYAYLI
AHCGASYLSS KTFRPCRRLK LVGERLTKSV VDQWRHIDTL INAYGPAENS VLSTLGHIKR
DESPHPSIGK GCGTVTWIVS TQRGGLASIG EVGELWVEGP LVAKGYLNDP GMTKEKFIRN
PPWLLQGAPG ISGRVGLLYK TGDLVRYDNT GSLHFVGRMD DQVKIRGQRI ELGEVEVHVG
QSIVSIESAE VQVVADVVTP RGYSDPILIA FISLYRNSQG HGENGGASRR ESKNHDVNDS
NNGHGNGNDN SGVYQQESLL LAASHSIRMS RDKISSKLPS YMVPSVCLLL DAFPMTSTGK
IDRRTLRRLA SCMTREQLAS ENMLHDRPVY RHPSTVAEKQ LQKLWSEVLG LEPRLISADD
SFLQIGGDSI QAMRLIGAAR EQGLALTVAD VLGHHSMSRL AQILESTKPE LGMLEPVEPF
SLLPKDVRVD DITPLTETKH GNMNAREGIS DIFPVTDLQT SYISAAMQDP PSSCSIFYLT
LPGQIAVEDL VKACEFVWSQ LDALRVAFVE YNGRYWQVIP TSSATPARIS IHRLDADESN
EKGSLHDEVY RKALMTSLRL GTTYTEFMIS RSPSQLRLGI RMTHAQYDGI SFGELVAMMS
AHLAGRTSMP ARSSPTIFEF ALAHEAESLK YWRNVLQGSQ PLVLAPRVPS PLTIGQDAAT
SPTSSSTRSL KTTGSSTRHL TRQGSPLLRT LSRMRYALSK VTTSTDVVFG FVVSGRAALQ
HNLSNVFGPC VNVVPIRASI ASADMSLTQL SLDLQQQRMR GRGSKLALAQ TSSGSAPTGP
KTRVITAALS RSKTLTSSRR STWTTSLPKL HAFPEEDQVD DAEVLYRPMR SRGGEVGSAR
GRCVGTW
//
